FERAYE ESEN GÜRSEL, IRAZ AKIŞ AKAD, Ahmet MENGİ, KEMAL ÖZDEM ÖZTABAK, Hanefi DURAK

Determination of BMP-15, BMPR-1B and GDF-9 Gene Mutations of the Indigenous Sheep Breeds in Turkey

Koyun yetiştiriciliğinde çoklu gebelik yetiştiriciler tarafından geliştirilmesi istenilen bir özelliktir. Bu çalışmada Türkiye’deki yerli ırk koyunlar arasında yer alan Sakız, Kıvırcık, İvesi ve İmroz ırkı koyunlarda çoklu gebeliği etkilediği ileri sürülen BMP-15, BMPR-1B ve GDF-9 genlerindeki mutasyonlar araştırılması amacıyla yapılmıştır. Bu amaçla her bir ırktan birbiri ile akraba olmayan 50 adet hayvan kullanılmıştır. Tüm hayvanlarda kan toplanması veDNA izolasyonun ardından, BMP-15, BMPR-IB ve GDF-9 genlerindeki hedef bölgeler polimeraz zincir reaksiyonu (PCR) ile çoğaltılmıştır. BMP-15 geninde yer alan FecXI FecXH FecXG FecXB mutasyonları, BMPRIB geninde yer alan FecB mutasyonu ve GDF-9 geninde yer alan FecGH mutasyonuları restriksiyon parçacık uzunluğu polimorfizmi (restriction fragment length polymorphism- RFLP) metodu kullanılarak belirlenmiştir. Analizi yapılan tüm koyun ırklarında BMP- 15 geninde yer alan FecXI (AA), FecXH (AA), FecXB (BB), FecB (AA), mutasyonlarında genotipler homozigot bulunmuşken sadece FecXG (AB) ve GDF-9 geninde yer alan FecGH (AB) mutasyonlarındaki genotipler heterozigot bulunmuştur. Yapılan çalışmalarda heterozigot genotiple çoklu doğum arasında pozitif bir korelasyon olduğu ileri sürülmektedir. Ancak Sakız ırkı koyunların bir batıdaki ortalama doğum sayısı 2 ve üzeri olmasına karşın çalışmanın sonucunda bunun incelenen genlerdeki mutasyonlardan kaynaklanmadığı ileri sürülebilir. Daha sonra yapılacak çalışmalarda özellikle sakız ırkı koyunlarda çoklu doğumun genetik olarak açıklanması amacıyla farklı genlerin incelenmesinin gerekli olduğu sonucuna varılmıştır.

Anahtar Kelimeler:

koyun, koyun ırkları, dölverim performansı, kısıtlı fragment uzunluğu polimorfizmi, genler, dişi döl verimi, genom, heterozigot, mutasyon, yerli çiftlik hayvanları, ovulasyon oranı, polimeraz zincir reaksiyonu, tespit etmek, Kıvırcık, DNA, Sakız, İvesi

Türkiye’deki Yerli Koyun Irklarında BMP-15, BMPR-IB ve GDF-9 Genlerindeki Mutasyonların Belirlenmesi

The high ovulation rate and fertility are very important production traits in sheep production. The aim of the study was to determine the BMP-15, BMPR-1B and GDF-9 gene mutations which are related with the high ovulation with high ovulation rate in Chios, Kivircik, Awassi and Imrose sheep breeds. Fifty sheep from each breed were provided for this study. Blood samples for all the animals were collected in sterile-2 ml tubes containing EDTA. Genomic DNAs were isolated using a standard salt-out method. Thereafter target sites were amplified with polymerase chain reaction (PCR). FecXI, FecXH, FecXG, FecXB mutations in BMP-15 gene, FecB mutation in BMPR-IB gene and FecGH mutation in GDF-9 gene were determined by restriction fragment length polymorphism (RFLP) method. All individuals were non-carriers for these mutations, except FecXG mutation in BMP-15 gene. The animals analyzed in this study were found to be heterozygous carriers for FecXG mutation. There may be other genes affecting fertility in Anatolian breeds. For the genetically explanation of multiple lambing, especially in Chios sheep, it was concluded that different genes should be investigated in the further studies.

Keywords:

sheep, sheep breeds, reproductive performance, restriction fragment length polymorphism, genes, female fertility, genomes, heterozygotes, mutations, native livestock, ovulation rate, polymerase chain reaction, determination, Kivircik, DNA, Chios, Awassi,

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