İBRAHİM ŞEKER, ÖZGE ÖZMEN, BENGİ ÇINAR KUL, Okan ERTUĞRUL

Polymorphism in Melatonin Receptor 1A (MTRN1A) Gene in Chios, White Karaman and Awassi Sheep Breeds [1]

Bu çalışma Sakız, Akkaraman ve İvesi ırkı koyunlarda melatonin reseptör 1A (MTNR1A) gen polimorfizminin incelenmesi amacıyla yapılmıştır. Çalışmada MTNR1A geni ekzon II bölgesi çoğaltılmış ve toplam 138 örnekte (48 adet Sakız, 40 adet Akkaraman ve 50 adet İvesi) 824 bç’lik tek PZR ürün elde edilmiştir. Elde edilen PCR ürünleri MnlI ve RsaI enzimleri ile kesilmiştir. MnlI enzim kesimi sonucu iki allel belirlenmiştir. İncelenen ırklarda Mm (303bp, 236bp/67bp) ve MM (236bp/67bp, 236bp/67bp) olmak üzere iki genotip saptanmıştır. Sakız, Akkaraman ve İvesi ırkları için allel frekansları; M alleli için sırasıyla 0.90, 0.80 ve 0.84; m alleli için sırasıyla 0.10, 0.20 ve 0.16 bulunmuştur. Genotipik frekanslar ise MM genotipi için sırasıyla 0.80, 0.60 ve 0.68 bulunurken Mm genotipi için 0.20, 0.40 ve 0.32 bulunmuştur. RsaI enzim kesimi için her üç ırkta da polimorfizm saptanmamıştır.

Anahtar Kelimeler:

koyun, genetik polimorfizm, genler, koyunlar, İvesi, Sakız, alleller, koyun ırkları, Akkaraman, genotip, melatonin, polimeraz zincir reaksiyonu, kısıtlı fragment uzunluğu polimorfizmi

Sakız, Akkaraman ve İvesi Koyun Irklarında Melatonin Reseptör 1A (MTRN1A) Gen Polimorfizmi

The aim of this study was to investigate polymorphism of the melatonin receptor 1A gene (MTNR1A) in Chios, White Karaman and Awassi sheep breed. A large fragment of the exon II of the MTNR1A gene was amplified and uniform fragment of 824 bp was obtained in 138 ewes (48 Chios, 40 White Karaman and 50 Awassi) of three breeds. The PCR products was digested with restriction endonuclease MnlI and RsaI , and genetic polymorphism was detected by PCR-RFLP. Biallelic polymorphism was found with restriction endonuclease MnlI and two genotypes were detected Mm (303bp, 236bp/67bp) and MM (236bp/67bp, 236bp/67bp). Allelic frequency for Chios, White Karaman and Awassi breeds was 0.90, 0.80 and 0.84 respectively for M allele; 0.10, 0.20 and 0.16 respectively for m allele, while genotypic frequency was 0.80, 0.60 and 0.68 for MM and 0.20, 0.40 and 0.32 for Mm respectively. No polymorphism at the RsaI cleavage sites was detected in three sheep breeds.

Keywords:

sheep, genetic polymorphism, genes, ewes, Awassi, Chios, alleles, sheep breeds, Akkaraman, genotypes, melatonin, polymerase chain reaction, restriction fragment length polymorphism,

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