MUHAMMET ALAN, YUNUS ÇETİN, Said SENDAG, HASAN ALTAN AKKAN, MEHMET KARACA

Seroprevalence of Antibodies Against Neospora caninum in Cows in Van Province

Bu çalışmada Van yöresindeki ineklerde Neospora caninum seroprevalansının cELISA ile belirlenmesi amaçlanmıştır. Çalışmanın materyali olarak; Yerli Irklar, Holştayn, Simental, Esmer ve bunların melezlerinden oluşan ve önceden en az bir kez doğum yapmış 450 inek kullanıldı. Her bir hayvanın jugular venasından cam tüplere yaklaşık 7 ml kan örneği alındı. Kan örneklerinden elde edilen serumlar -20°C de depolandı ve son örnekleme işleminden iki ay sonra ticari bir N. caninum antikor test kiti (cELISA; VMRD, Şti.) kullanılarak işlendi. Inhibisyon değeri ≥%30 olanlar seropozitif ve

Anahtar Kelimeler:

hastalık yaygınlığı, melez ırklar, hastalık tetkiki, epidemiyolojik araştırmalar, epidemiyoloji, inek, sığır, antikor, Türkiye, Simmental, Neospora caninum, Alacalı Sığır, Amerikan Esmer Irkı, yerli çiftlik hayvanları, neosporosiz, seroprevalans, süt sığırı, süt ineği

Van Yöresinde İneklerde Neospora caninum Antikorlarının Seroprevalansı

The objective of this study was to determine the seroprevalence of Neospora caninum by cELISA among cows in Van province in Turkey. As material; 450 cows consisting of Brown Swiss, Simmental, Holstein, native species and crossbreds of these and given birth before at least one time were used. Approximately 7 ml blood sample was drawn from the jugular vein of each animal into the plain tubes. Sera obtained from the blood samples were stored at -20°C and processed using a commercial N. caninum antibody test kit (cELISA; VMRD, Inc.) after two mounts from the last sampling. Inhibitions ≥30% were interpreted as seropositive and inhibitions <30% were interpreted as seronegative. Seroprevalence of antibodies against N. caninum in cows in Van province was 4.88% as a general average. Seropositive sample rate of the central districts was higher than the rate of central villages, 10.66% vs. 3.73%. On the other hand, seropositive sample rate of the animals came to the animal hospital from the central villages was higher than the rate of animals sampled in their places, 8.06% vs. 2.87%. In conclusion, the seroprevalence of N. caninum among cows in Van province in Turkey is lower or higher than those of some other provinces in Turkey and countries or regions in the world. The reason of this may be different cattle breeds, breeding systems and animal traffic among regions or countries. Along with main control measurements to contain N. caninum infection, diagnosing and culling of seropositive animals would give helpful results.

Keywords:

disease prevalence, crossbreds, disease surveys, epidemiological surveys, epidemiology, cows, cattle, antibodies, Turkey, Simmental, Neospora caninum, Holstein-Friesian, American Brown Swiss, native livestock, neosporosis, seroprevalence, dairy cattle, dairy cows,

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Frossling J: Epidemiology of Neospora caninum infection in cattle. Doctoral thesis, Swedish University of Agricultural Sciences, Uppsala, 2004.
Sevgili M, Altaş MG: Neosporosis in cattle. Fırat Üniv Sağlık Bil Derg, 20 (1): 79-83, 2006.
Reichel MP: Neospora caninum infections in Australia and New Zealand. Aust Vet J, 78 (4): 258-261, 2000.
Ogawa L, Freire RL, Vidotto O, Gondim LFP, Navarro IT: Occurrence of antibodies to Neospora caninum and Toxoplasma gondii in dairy cattle from the northern region of the Parana State, Brazil. Arq Bras Med Vet Zoo, 57 (3): 312-316, 2005.
Haddad JPA, Dohoo IR, VanLeewen JA: A review of Neospora caninum in dairy and beef cattle - A Canadian perspective. Can Vet J, 46, 230-243, 2005.
Medina L, Cruz-Vazquez C, Quezada T, Morales E, Garcia-Vazquez Z: Survey of Neospora caninum infection by nested PCR in aborted fetuses from dairy farms in Aguascalientes, Mexico. Vet Parasitol, 136, 187-191, 2006.
Sotiraki S, Brozos C, Samartzi F, Schares G, Kiossis E, Conraths FJ: Neospora caninum infection in Greek dairy cattle herds detected by two antibody assays in individual milk samples. Vet Parasitol, 152, 79-84, 2008.
Wouda W, Moen AR, Schukken YH: Abortion risk in progeny of cows after a Neospora caninum epidemic. Theriogenology, 49, 1311-1316, 1998.
Anderson ML: Infectious causes of bovine abortion during mid- to late-gestation. Theriogenology, 68, 474-486, 2007.
Bartels CJM, Wouda W, Schukken YH: Risk factors for Neospora caninum-associated abortion storms in dairy herds in the Netherlands (1995 to 1997). Theriogenology, 52, 247-257, 1999.
Wouda W, Bartels CJM, Moen AR: Characteristics of Neospora caninum-assoclated abortion storms in dairy herds in the netherlands (1995 to l997). Theriogenology, 52, 233-245, 1999.
Andrianarivo AG, Anderson ML, Rowe JD, Gardner IA, Reynolds JP, Choromanski L, Conrad PA: Immune responses during pregnancy in heifers naturally infected with Neospora caninum with and without immunization. Parasitol Res, 96, 24-31, 2005.
Ferre I, Aduriz G, del-Pozo I, Regidor-Cerrillo J, Atxaerandio R, Collantes-Fernandez E, Hurtado A, Ugarte-Garagalza C, Ortega-Mora LM: Detection of Neospora caninum in the semen and blood of naturally infected bulls. Theriogenology, 63, 1504-1518, 2005.
Serrano E, Ferre I, Osoro K, Aduri z G, Mateos-Sanz A, Martinez A, Atxaerandio R, Hidalgo CO, Ortega-Mora LM: Intrauterine Neospora caninum inoculation of heifers. Vet Parasitol, 135, 197-203, 2006.
Lopez-Gatius F, Lopez-Bejar M, Murugavel K, Pabon M, Ferrer D, Almeria S: Neospora-associated abortion episode over a 1-year period in a dairy herd in north-east Spain. J Vet Med B, 51, 348-352, 2004.
Lopez-Gatius F, Santolaria P, Almeria S: Neospora caninum infection does not affect the fertility of dairy cows in herds with high incidence of Neospora-associated abortions. J Vet Med B, 52, 51-53, 2005.
Lopez-Gatius F, Pabon M, Almeria S: Neospora caninum infection does not affect early pregnancy in dairy cattle. Theriogenology, 62, 606-613, 2004.
Stahl K, Bjorkman C, Emanuelson U, Rivera H, Zelada A, Moreno-Lopez J: A prospective study of the effect of Neospora caninum and BVDV infections on bovine abortions in a dairy herd in Arequipa. Peru Prev Vet Med, 75 (3-4): 177-188, 2006.
Lopez-Gatius F, Santolaria P, Yaniz JL, Garbayo JM, Almeria S: The use of beef bull semen reduced the risk of abortion in Neospora-seropositive dairy cows. J Vet Med B, 52, 88-92, 2005.
Collantes-Fernandez E, Rodriguez-Bertos A, Arnaiz-Seco I, Moreno B, Aduriz G, Ortega-Mora LM: Influence of the stage of pregnancy on Neospora caninum distribution, parasite loads and lesions in aborted bovine fetuses. Theriogenology, 65, 629-641, 2006.
Hall CA, Reichel MP, Ellis JT: Neospora abortions in dairy cattle: Diagnosis, mode of transmission and control. Vet Parasitol, 128, 231-241, 2005.
Varcasia A, Capelli G, Ruiu A, Ladu M, Scala A, Bjorkman C: Prevalence of Neospora caninum infection in Sardinian dairy farms (Italy) detected by iscom ELISA on tank bulk milk. Parasitol Res, 98, 264-267, 2006.
. Chanlun A, Emanuelson U, Chanlun S, Aiumlamai S, Bjorkman C: Application of repeated bulk milk testing for identification of infection dynamics of Neospora caninum in Thai dairy herds. Vet Parasitol, 136, 243-250, 2006.
Oncel T, Biyikoglu G: Neosporosis caninum in dairy cattle in Sakarya, Turkey. Uludag Univ J Fac Vet Med, 22 (1-2-3): 87-89, 2003.
Aktas M, Saki CE, Altay K, Simsek S, Utuk AE, Koroglu E, Dumanli N: Survey of Neospora caninum in cattle in some provinces in the Eastern Anatolian Region. Acta Parasitol Turcica, 29 (1): 22-25, 2005.
Kurtdede A, Kuplulu S, Ural K, Cingi CC, Guzel M, Karakurum MC, Haydardedeoglu AE: Serodiagnosis of bovine neosporosis with immunocomb assay in Ankara region. Ankara Univ Vet Fak Derg, 53, 207-209, 2006.
Ica A, Yildirim A, Duzlu O, Inci A: Seroprevalence of Neospora caninum in cattle in the region of Kayseri. Acta Parasitologica Turcica, 30 (2): 92-94, 2006.
Akca A, Gokce HI, Guy CS, McGarry JW, Williams DJL: Prevalence of antibodies to Neospora caninum in local and imported cattle breeds in the Kars province of Turkey. Res Vet Sci, 78, 123-126, 2005.
SAS: User’s Guide Statistics. 5 th ed., SAS Iinit, Inc, Cary, NC, 1985.
Baszler TV, Adams S, Vander-Schalie J, Mathison BA, Kostovic M: Validation of a commercially available monoclonal antibody-based competitive-inhibition Enzyme-Linked Immunosorbent Assay for detection of serum antibodies to Neospora caninum in Cattle. J Clin Microbiol, 39 (11): 3851-3857, 2001.
Baszler TV, Knowles DP, Dubey JP, Gay JM, Mathison BA, McElwain TF: Serological diagnosis of bovine neosporosis by Neospora caninum monoclonal antibody-based competitive inhibition Enzyme-Linked Immunosorbent Assay. J Clin Microbiol, 34 (6): 1423-1428, 1996.
Wu JTY, Dreger S, Chow EYW, Bowlby EE: Validation of 2 commercial Neospora caninum antibody enzyme linked immunosorbent assays. Can J Vet Res, 66, 264-271, 2002.
Koiwai M, Hamaoka T, Haritani M, Shimizu S, Zeniya Y, Eto M, Yokoyama R, Tsutsui T, Kimura K, Yamane I: Nationwide seroprevalence of Neospora caninum among dairy cattle in Japan. Vet Parasitol, 135, 175-179, 2006.