ABDULLAH KAYA, HASAN İÇEN, Nevin TUZCU, AYNUR ŞİMŞEK, Simten YEŞİLMEN, AKIN KOÇHAN, İBRAHİM HALİL YILDIRIM, Adil ALAN

Comparison of Tuberculin Skin Test, IFN-γ Assay, Real Time PCR and Lateral Flow Rapid Test in Diagnosis of Field Outbreaks of Bovine Tuberculosis

Sığır tüberkülozu; direkt temas, solunum yolu, pastörize edilmemiş süt ve süt ürünleri, çiğ ya da az pişmiş etlerin alınmasıyla insanlara da bulaşabilen önemli bir zoonoz hastalıktır. Yalnızca klinik bulgulara bakarak tüberkülozu teşhis etmek zor olabilmektedir. Genellikle tüberkülozun tanısı sahada tüberkülin testiyle konulmaktadır. Mikrobiyoloj ik muayene amacıyla kraşe ve diğer vücut sıvıları temin edilmektedir. Polymerase chain reaction (PCR) yöntemleri de kullanılabilmektedir. Tanı amaçlı kan testleri arasında lenfosit proliferasyon testi, interferon gamma (IFN-γ) testi ve enzyme-linked immunosorbent assay (ELISA) bulunmaktadır. Bu çalışmada 50 hayvan tuberculin skin test (TST), lateral flow rapid test, IFN-γ assay ve gerçek zamanlı PCR kullanılarak test edilmiştir. Hayvanlar 3-5 yaş arasındaki tüberküloz şüpheli 178 sütçü sığır arasından rastgele seçilmiştir. TST sonuçlarına göre 50 sığırdan 45i pozitifken, IFN-γ testine göre 31i pozitif bulunmuştur. TB lateral flow rapid testte 28 pozitif, gerçek zamanlı PCR testinde ise 9 pozitif sonuç bulunmuştur. Çalışmamızda farklı testlerin uygulanmasının amacı tüberkülin skin test, IFN-γ assay, TB lateral flow rapid test ve gerçek zamanlı PCR testinin Türkiyede karşılaşılan sığır tüberkülozundaki duyarlılıklarının karşılaştırılmasıdır.

Sahada Görülen Sığır Tüberkülozunun Tanısında Kullanılan Tuberculin Skin Test, IFN-γ Assay, Gerçek Zamanlı PCR ve Lateral Flow Rapid Testlerinin Karşılaştırılması

Bovine tuberculosis is an important zoonotic disease transmitted by direct contact, respiratory pathway, ingestion of unpasteurised milk and milk product, raw or undercooked meat. Tuberculosis can be difficult to diagnose based only on the clinical signs. Tuberculosis is usually diagnosed in the field with the tuberculin skin test. Sputum and other body fluids may be collected for microbiological examination. Polymerase chain reaction (PCR) methods have also been described. Diagnostic blood tests include the lymphocyte proliferation assay, the interferon gamma (IFN-γ) assay, and enzyme-linked immunosorbent assays (ELISA). In this study a total of 50 animals were tested by using tuberculin skin test (TST), lateral flow rapid test, IFN-γ assay and real time PCR. The animals were selected randomly among 178 cattle in dairy farms with the aged between 3-5 years and suspected of having tuberculosis. Forty five cattle were positive out of 50 for TST while 31 for reactive by the IFN-γ assay and 28 for rapid test and 9 for real time PCR. The purpose behind such variable as age was to compare sensitivity of tuberculin skin test, the IFN-γ assay and TB lateral flow rapid test and real time PCR examination for the diagnosis of field outbreaks of bovine tuberculosis in Turkey.

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