Levent ŞAHİN, Hilal ŞAHİN, Feride KARAHAN, SEMİR GÜL, LEYLA BAHAR, Mehmet GÜL, MÜZEYYEN GÜLNUR ÖZAKŞİT

Can Melatonin Protect the Endometrium from the Adverse Effects of Caerulein?

Serulein ile indüklenmiş akut pankreatiti (AP)nin uterusa etkisi ve melatonin uygulamasının muhtemel uterus koruyucu etkileri araştırıldı. Yirmi sekiz rat dört gruba ayrıldı: (1) Kontrol grubu (n = 7); (2) melatonin grubu (n = 7); (3) serulein grubu (n = 7); (4) melatonin + serulein grubu (n = 7). AP, genç dişi hayvanlara saatte bir (50 μg/kg) dozunda 4 kez verilen intraperitonal serulein enjeksiyonu ile indüklenmiştir. Melatonin (20 mg/kg) AP indüksiyonundan 30 dakika önce intraperitonal enjeksiyon yolu ile verildi. Ratlar seruleinin son enjeksiyonundan 12 saat sonra sakrifiye edildi ve uterusları histopatoloj ik değerlendirme için alındı. Ortalama vücut ağırlığı ve uterusun ıslak ağırlıkları kaydedildi. Endo-miyometriyumdaki histoloj ik değişikliklerin derecesi, ödem, kanama, nekroz, lökosit infiltrasyonu, endometriyal proliferasyon ve endometrial kalınlık skorlaması için H-Puan yöntemi kullanıldı. Her grupta tedaviden sonra ortalama vücut ağırlığında anlamlı bir farkın olmadığı gözlendi. Kontrol ve serulein verilen ratlar arasındaki uterus ıslak ağırlıkları anlamlı derecede farklı (P

Melatonin Seruleinin Olumsuz Etkilerine Karşı Endometriyumu Koruyabilir mi?

We investigated the effects of a caerulein-induced acute pancreatitis (AP) on uterus and possible uterine protective effects of melatonin administration. Twenty-eight animals were divided into four groups: (1) control group (n = 7); (2) melatonin group (n = 7); (3) caerulein group (n = 7); (4) melatonin + caerulein group (n = 7). AP was induced by 4 intraperitoneal inj ection of caerulein given hourly (50 μg/kg) into young female animals. Melatonin (20 mg/kg) was given via intraperitoneal inj ection 30 min prior to the induction of AP. The rats were sacrificed by decapitation 12 h after the last inj ection of caerulein and their uterus were taken for histopathological evaluation. Mean body weight and uterine wet weight was recorded. The H-Score method was used to score the degree of histological changes of endo-myometrium edema, hemorrhage, necrosis, leucocyte infiltration, endometrial proliferation and endometrial thickness. There was no significant difference in the mean body weight observed after treatment in each group. The uterine wet weight differences between the control and caerulein given rats were significant (P<0.01). The endometrial thickness, edema, hemorrhage, necrosis and leucocyte infiltration of the caerulein group was significantly higher than the control and melatonin groups (P<0.01). It was observed that preteratment with melatonin normalized histological abnormalities and significantly reduced uterine wet weight as compared with the caerulein only group. Melatonin application may play an important role in the prophylaxis of uterine endometrium arising from adverse effects of caerulein.

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