HATİCE ŞANLIDERE ALOĞLU, EZGİ DEMİR ÖZER, Zübeyde ÖNER, Hasan Basri SAVAŞ, Efkan UZ

Investigation of a Probiotic Yeast as a Cholesterol Lowering Agent on Rats Fed on a High Cholesterol Enriched Diet

Yüksek kolesterol asimilasyon yeteneğine sahip probiyotik maya Cryptococcus humicola M5-2 suşu, geleneksel olarak üretilmiş bir peynirden izole edilmiş ve in vivo ortamda incelenmiştir. Çalışmada, C. humicola M5-2 suşunun in vivo ortamda kolesterolü asimile etme yeteneği incelenmiş ve kolesterolce zengin diyetle beslenen sıçanlarda bu suş ile beslemenin serum toplam kolesterol, HDL/LDL kolesterol ve trigliserit seviyelerine etkisi belirlenmiştir. C. humicola nın besiyeri içersindeki kolesterolü asimile etme oranı %73.33tür. Hayvanların beslenmesinde suş iki farklı dozda kullanılmıştır (yüksek ve düşük doz, sırasıyla %2 ve %0.1 liyofilize suş içermektedir). Deneme grupları karşılaştırıldığında, düşük doz besleme grubu daha olumlu sonuçlar göstermiştir. Serum analiz sonucuna göre trigliserit ve toplam kolesterol düzeyi sırasıyla %25 ve %1.34 oranında düşme göstermiştir. Özellikle trigliserit düzeyindeki düşme oranı çoğu in vivo çalışmada rastlanmamıştır. Elde edilen suşun fermente gıdalar ile beraber tüketiminin sağlık üzerine iyileştirici etkiler yaratacağı düşünülmektedir.

Probiyotik Mayanın Yüksek Kolesterol İçeren Diyetle Beslenen Sıçanlarda Kolesterolü Düşürücü Ajan Olarak Kullanılabilirliğinin Araştırılması

Probiotic yeast, Cryptococcus humicola M5-2 strain, which has high cholesterol assimilation feature and isolated from traditionally produced cheese, was investigated in vivo. This study was conducted to determine cholesterol assimilation ability of C. humicola M5-2 in vivo and specify the effect of the strain on serum total cholesterol, HDL/LDL cholesterol and triglycerides levels in rats fed on cholesterol-enriched diet. C. humicola had the ability to assimilate cholesterol at the rate of 73.33% in media. The strain was used with two different concentrations in animal feed (high and low doses containing 2% and 0.1% lyophilized strains, respectively). When the treatment groups were compared, low dose feeding group had the positive results in terms of testing values. According to the results of serum analysis, triglyceride and total cholesterol level were decreased by 25% and 1.34% respectively. Especially, decreasing the percentage rate of triglyceride has not obtained in other in vivo studies. It is thought that health promoting effect will be possible when the obtained isolate is consumed with fermented foods.

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