Hamdullah SEÇKİN, Kerem ÖZDEMİR, Şükrü ÖNALAN, Metin ERTAŞ, Erdal ÖĞÜN

Aras Nehrinin Belirli Noktalarından Alınan Sediment Örneklerinden Streptomyces Bakterilerinin İzolasyonu Teşhisi ve Moleküler Karakterizasyonu

Aras Nehri coğrafik ve ekolojik anlamda çok önemli bir yere sahiptir. Aras Nehrindeki Streptomyces çeşitliliğinin tespit edilmesi, izolasyonu, teşhisi ve karakterizasyonunun, mikrobiyolojik ve moleküler biyoloji ve genetik alanlarında bilim dünyasına katkı sağlayacağı düşünülmektedir. Streptomyces türleri bilim dünyası için çok önemli bir yere sahiptir, çünkü bu bakteri türlerinin ürettiği başta antibiyotikler olmak üzere birçok önemli madde bulunmaktadır. Aras Nehrinin geçtiği Erzurum Horasan, Kars Karakurt ve Iğdır Tuzluca bölgelerinden alınan numuneler Streptomyces çeşitliliği bakımından incelendi. Belirlenen lokalitelerden alınan sediment örneklerinin pH ve nem oranları tespit edildi. Bennet’s Agar ve M65 besiyerleri kullanılarak izolasyon, saflaştırma ve renk grubu belirleme çalışması yapıldı. Bu bakteri gruplarının teşhisi amacıyla biyokimyasal, fenotipik ve moleküler testler uygulandı. Renk grubuna göre belirlenen izolatların Spor zincir morfolojisi görüntülendi (SEM). Mikrobiyolojik olarak izole edilecek bakterilerin 16 S rDNA gen bölgelerinin sekans analizi gerçekleştirildi. Bu işlemin ardından elde edilen diziler Genbank verileri ile karşılaştırılarak izole edilen bakterilerin moleküler yakınlık ve benzerlik oranları elde edildi.

Anahtar Kelimeler:

Aras Nehri, Streptomyces, DNA izolasyonu, 16 S rDNA, Sekans analizi, Aras River, Streptomyces, DNA isolation, 16 S rDNA, Sequence analysis

Isolation, Identification and Molecular Characterization of Streptomyces Bacteria from Sediment Samples Taken from Certain Points of Aras River

Aras river has a very important place in terms of geographical and ecological sense. It is thought that the detection, isolation, diagnosis and characterization of Streptomyces diversity in the Aras river will contribute to the scientific world in the fields of microbiological and molecular biology and genetics. Streptomyces species have a very important place for the scientific world, because there are many important substances produced by these bacterial species, especially antibiotics. Samples taken from Erzurum Horasan, Kars Karakurt and Iğdır Tuzluca regions where Aras river passes were examined in terms of Streptomyces diversity. The pH and moisture ratios of the sediment samples taken from the determined localities were determined. Isolation, purification and color group determination studies were performed using Bennet's Agar and M65 media. Biochemical, phenotypic and molecular tests were applied to diagnose these bacterial groups. Spore chain morphology of the isolates determined according to the color group was visualized (SEM). Sequence analysis of 16 S rDNA gene regions of bacteria to be isolated microbiologically was performed. The sequences to be obtained after this process were compared with the genbank data, and the molecular affinity and similarity rates of the isolated bacteria were obtained.

Keywords:

Aras River, Streptomyces, DNA isolation, 16 S rDNA, Sequence analysis,

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