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İnfeksiyöz Deve (Camelus dromedarius) Keratokonjonktivitis Olgularında Moraxella bovoculi'nin İdentifikasyonu ve Antimikrobiyal Duyarlılıklarının Belirlenmesi

İnfeksiyöz keratokonjonktivit (İKC) sığır ve koyunlarda ekonomik kayıplara neden olan bir göz hastalığıdır. Hastalık etkenleri genel olarak Moraxella bovis ve M. ovis olarak bilinirken 2007'de IKC'den de sorumlu türler arasına M. bovoculi’de tanımlanmıştır. Bu çalışmanın amacı konjonktival hiperemi, oküler ağrı, fotofobi ve lakrimasyon semptomları gösteren develerde Moraxella spp. varlığının saptanması ve antimikrobiyal duyarlılık profillerindeki farklılıkları belirlemektir. Aydın yöresinde 30 adet deve’ye (Camelus dromedarius) ait sağ ve sol gözlerden bilateral (n= 60 örnek) konjonktival svap örnekleri nazikçe toplandı. Moraxella spp. (6/60; %10) suşları sürüntü örneklerinden fenotipik ve genotipik yöntemlerle izole edildi. Biyokimyasal olarak ilk değerlendirme nitrat redüksiyon ve jelatinaz sonuç negatif olması yönünden M. ovis ve M. bovoculi (M. bovis negatif) ile uyumluluk gösterdi. Ayrıca, 357F ve 1492R evrensel primerleri kullanarak 16S rRNA PCR gerçekleştirildi ve nükleotit sekansı yapıldı. Sanger sekanslama ile izolatların Moraxella bovoculi (Moraxella bovoculi suşu 3709'a %98-99 benzerlik Access. No: GU181221.1) olduğu doğrulandı. İzolatlarda eritromisin (%100), amoksisilin-klavulanik asit, penisilin, siprofloksasin ve tetrasiklin (%67) gibi yaygın antibiyotiklere direnç, sefotaksim, gentamisin ve imipenem’e (%100) duyarlılık tespit edildi. M. bovoculi suşu develerin göz infeksiyonlarında ülkemizde daha önce rapor edilmemiştir. Bu nedenle çalışmamız develerin göz infeksiyonlarında M. bovoculi'nin varlığını doğrulamaktadır ve develerin göz infeksiyonlarından izole edilebileceğine vurgu yapmaktadır.

Anahtar Kelimeler:

Camelus dromedarius, Moraxella bovoculi, dizileme, göz hastalığı, polimeraz zincir reaksiyonu.

Identification of Moraxella bovoculi in Infectious Camel (Camelus dromedarius) Keratoconjunctivitis Cases and Determination of Antimicrobial Susceptibility

Infectious keratoconjunctivitis (ICC) is an eye disease that causes economic losses in cattle and sheep. The causative agents of the disease are commonly known as Moraxella bovis and M. ovis. In 2007, M. bovoculi was identified among the species responsible for IKC. The aim of this study was to detect Moraxella spp. in camels with conjunctival hyperemia, ocular pain, photophobia, and lacrimation symptoms. The aim of this study is to detect the presence of antimicrobial susceptibility profiles and to determine the differences in antimicrobial susceptibility profiles. Bilateral (n= 60 samples) conjunctival swab samples were gently collected from the right and left eyes of 30 camels (Camelus dromedarius) in the Aydin region. Moraxella spp. (6/60; 10%) strains were isolated from swab samples by phenotypic and genotypic methods. Biochemically, the initial evaluation showed compatibility with M. ovis and M. bovoculi (M. bovis negative) in terms of nitrate reduction and gelatinase negative result. Also, 16S rRNA PCR was performed using 357F and 1492R universal primers, and nucleotide sequencing was performed. The isolates were confirmed to be M. bovoculi (98-99% similarity to M. bovoculi strain 3709 Access. No: GU181221.1) by Sanger sequencing. Resistance to erythromycin (100%), amoxicillin-clavulanic acid, penicillin, ciprofloxacin, and tetracycline (67%) and susceptibility to cefotaxime, gentamicin, and imipenem (100%) were detected in the isolates. M. bovoculi strain has not been previously reported in camel eye infections in our country. Therefore, our study confirms the presence of M. bovoculi in camel eye infections and emphasizes that camels can be isolated from eye infections.

Keywords:

Camelus dromedarius, eye disease, Moraxella bovoculi, polymerase chain reaction, sequencing,

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