Soğukta bekletme süresinin uzamanasının Tenebrio molitor’un toplam lipid miktarına ve ergin yamyamlığına etkisi
Tenebrio molitor (Coleoptera: Tenebrionidae) larvalarının son yıllarda protein ve lipit içeriğinin yüksek olduğunun keşfedilmesi ile katkı yemi veya canlı yem olarak kitlesel olarak yetiştirilmektedir. Fakat üretim tesislerinde üreticilerin en sık karşılaştığı sorunlardan biri popülasyon yoğunluğu sonucu ortaya çıkan kanibalizmdir. Bu nedenle özellikle popülasyon çok yoğun olduğu dönemlerde üreticiler pupa aşamasında olan böcekleri kültürden ayırarak ya başka kaplarda ya da larva ve pupaları buzdolabı gibi soğutucularda bekletmektedirler. Daha sonra ihtiyaç olduğunda böcekleri buzdolabından çıkarıp kullanmaktadırlar. Fakat böcekler ektoterm canlılar olduklarından yaşam döngüleri aşırı derecede sıcaklığa bağımlıdır. Soğukta depolama yöntemi böceklerin raf ömrünü uzatmakla birlikte uzun süreler düşük sıcaklığa maruz bırakmak onların yaşam döngülerine hem zarar verebilir hem de içerdikleri yağ ve protein oranını önemli derecede etkileyebilir. Bu çalışmada soğukta depolamanın T. molitor larva, pupa ve erginlerinin toplam yağ miktarı, toplam yağ yüzdesi ve kanibalizm oranına etkileri değerlendirildi. Çalışmanın ilk aşamasında larvalar 100-190 mg (12-17. larval aşamadakiler) ağırlığa gelene kadar beslendi ve sonrasında rastgele seçilerek 10, 20 ve 30 gün boyunca soğuğa maruz bırakıldı. Çalışmanın ikinci aşamasında larvalar pupa olduktan sonra 10, 20 ve 30 gün boyunca soğuğa maruz bırakıldı. Sonra normal laboratuvar şartlarına alınarak gelişimlerinin tamamlanması beklendi ve yağ analizleri yapılarak, kanibalizm oranlarına bakıldı. Sonuç olarak soğuğa maruz kalma süresi uzadıkça beslenmemiş kontrol grubu larvalarda toplam yağ miktarı ve yüzdeleri düşmüş, beslenmemiş ve soğuğa maruz kalmış grubun ise artmış veya sabit kalmıştır. Ayrıca T. molitor erginlerinde beslenmedikleri zaman yani açlık ve susuzluk durumlarında kanibalizm görülmüştür.
The effect of prolonged cold storage period on total lipid content and adult cannibalism of Tenebrio molitor
With the discovery that the larvae of Tenebrio molitor (Coleoptera: Tenebrionidae) have a high protein and lipid content in recent years, they are grown in mass as additive feed or live feed. However, one of the most common problems faced by producers in production facilities is cannibalism, which occurs as a result of population density. For this reason, especially when the population is very dense, producers separate the insects that are in the pupa or larvae stage from the culture and keep them in coolers such as refrigerators. Then, when needed, they take the insects out of the refrigerator and use them. However, because insects are ectotherm organisms, their life cycles are extremely dependent on temperature. Although the cold storage method extends the shelf life of insects, exposing them to low temperatures for long periods can both damage their life cycle and significantly affect their lipid and protein content. In this study, the effects of cold storage on total lipid content, total lipid percentage and cannibalism rate of T. molitor larvae, pupae and adults were evaluated. In first stage of the study, the larvae were fed until they weighed 100-190 mg (larval stages 12-17). Afterwards, they were randomly selected and exposed to cold for 10, 20 and 30 days. In the second stage of the study, the larvae were exposed to cold for 10, 20 and 30 days after pupation. Then, they were put under normal laboratory conditions and their development was expected to be completed, and lipid analyzes were made and cannibalism rates were checked. As a result, as the duration of exposure to cold increased, the total lipid content and percentages decreased in the larvae of the unfed control group, while it increased or remained constant in the unfed and cold-exposed group. In addition, cannibalism was observed in T. molitor adults when they werent fed, that is, in cases of hunger and thirst.
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- Adámková, A., Adámek, M., Mlček, J., Borkovcová, M.,
Bednářová, M., Kouřimská, L., Skácel, J. &
Vítová, E. (2017). Welfare of the mealworm
(Tenebrio molitor) breeding with regard to nutrition
value and food safety. Potravinarstvo Slovak
Journal of Food Sciences, 11(1), 460-465. DOI:
10.5219/779.
- Adámková, A., Mlček, J., Adámek, M., Borkovcová, M.,
Bednářová, M., Hlobilová, V., Knížková, I. &
Juríková, T. (2020). Tenebrio molitor (Coleoptera:
Tenebrionidae) optimization of rearing conditions to
obtain desired nutritional values. Journal of Insect
Science, 20, 1-10. DOI: 10.1093/jisesa/ieaa100.
- Arbab, A. (2019). Effect of temperature on pupal
development in meal worm Tenebrio molitor L.
Indian Journal of Entomology, 81(4), 640-646. DOI:
10.5958/0974-8172.2019.00138.X.
- Aguila, J.R., Suszko, J., Gibbs, A.G. & Hoshizaki, D.K.
(2007). The role of larval fat cells in adult
Drosophila melanogaster. Journal of Experimantal
Biology, 210(6), 956-963. DOI: 10.1242/jeb.001586.
- Costa, S., Pedro, S., Lourenço, H., Batista, I., Teixeira, B.,
Bandarra, N.M., Murta, D., Nunes, R. & Pires, C.
(2020). Evaluation of Tenebrio molitor larvae as an
alternative food source. NFS Journal, 21, 57-64.
DOI: 10.1016/j.nfs.2020.10.001.
- Deruytter, D., Coudron, C.L. & Teerlinck, S. (2019).
Influence of crate size, oviposition time, number of
adults and cannibalism on the reproduction of
Tenebrio molitor. Journal of Insects as Food and
Feed, 5(4), 1-10. DOI: 10.3920/JIFF2019.0018.
- Dreassi, E., Cito, A., Zanfini, A., Materozzi, L., Botta, M.
& Francardi, V. (2017). Dietary fatty acids
influence the growth and fatty acid composition of
the yellow mealworm Tenebrio molitor (Coleoptera:
Tenebrionidae). Lipids, 52(3), 285-294. DOI:
10.1007/s11745-016-4220-3.
- Errico, S., Spagnoletta, A., Verardi, A., Moliterni, S.,
Dimatteo, S. & Sangiorgio, P. (2021). Tenebrio
molitor as a source of interesting natural
compounds, their recovery processes, biological
effects, and safety aspects. Comprehensive Reviwes
in Food Science and Food Safety, 21,148-197. DOI:
10.1111/1541-4337.12863.
- Finkel, A.J. (1948). The lipid composition of Tenebrio
molitor larvae. Physiological Zoology, 21(2), 111-
133.
- Folch, J., Lees, M. & Stanley, S.G.H. (1957). A simple
method for the isolation and purification of total
lipids from animal tissues. Journal of Biological
Chemistry, 226(1), 497-509. DOI: 10.1016/s0021-
9258(18)64849-5.
- Halloran, A., Muenke, C., Vantomme, P. & Van Huis, A.
(2014). Insects in the human food chain: global
status and opportunities. Food Chain, 4(2), 103-118.
DOI: 10.3362/2046-1887.2014.011.
- Ichikawa, T. & Kurauchi, T. (2009). Larval cannibalism and
pupal defense against cannibalism in two species of
Tenebrionid beetles. Zoological Science, 26(8), 525-
529. DOI: 10.2108/zsj.26.525.
- Irwin, J.T. & Lee, R.E.Jr. (2003). Cold winter
microenvironments conserve energy and improve
overwintering survival and potential fecundity of the
goldenrod gall fly, Eurosta solidaginis. Oikos,
100(1), 71-78. DOI: 10.1034/j.1600-
0706.2003.11738.x.
- Jones, L.D., Cooper, R.W. & Harding, R.S. (1972).
Composition of mealworm Tenebrio molitor larvae.
The Journal of Zoo Animal Medicine, 3(4), 34-41.
- Liu, C., Masri, J., Perez, V., Maya, C. & Zhao, J. (2020).
Growth performance and nutrient composition of
mealworms (Tenebrio molitor) fed on fresh plant
materials-supplemented diets. Foods, 9(2), 151.
DOI: 10.3390/foods9020151.
- Levie, A., Vernon, P. & Hance, T. (2005). Consequences of
acclimation on survival and reproductive capacities
of coldstored mummies of Aphidius rhopalosiphi
(Hymenoptera: Aphidiinae). Journal of Economical
Entomology, 98(3), 704-708.
- Marshall, K.E. & Sinclair, B.J. (2012). Threshold
temperatures mediate the impact of reduced snow
cover on overwintering freeze-tolerant caterpillars.
Naturwissenschaften, 99, 33-41.
- Mirzaeva, D.A., Khujamshukurov, N.A., Zokirov, B.,
Soxibov, B.O. & Kuchkarova, DKh. (2020).
Influence of temperature and humidity on the
development of Tenebrio molitor L. International
Journal of Current Microbiology and Applied
Sciences, 9(4), 3544-3559. DOI:
10.20546/ijcmas.2020.905.422.
- Mlček, J., Adámková, A., Adámek, M., Borkovcová, M.,
Bednářová, M. & Knížková, I. (2019). Fat from
Tenebrionidae bugs – sterols content, fatty acid
profiles, and cardiovascular risk indexes. Polish
Journan of Food and Nutrition Sciences, 69(3), 247-
254. DOI: 10.31883/pjfns/109666.
- Morales-Ramos, J.A., Rojas, M.G., Kay, S., Shapiro-lIan,
D.I. & Tedders, W.L. (2012). Impact of adult
weight, density, and age on reproduction of Tenebrio
molitor (Coleoptera: Tenebrionidae). Journal of
Entomological Science, 47(3), 208-220. DOI:
10.18474/0749-8004-47.3.208.
- Morales-Ramos, J.A., Rojas, M.G, Shelby, K.S. &
Coudron, T.A. (2015). Nutritional value of pupae
versus larvae of Tenebrio molitor (Coleoptera:
Tenebrionidae) as food for rearing Podisus
maculiventris (Heteroptera: Pentatomidae). Journal
of Economical Entomology, 109(2), 564-571. DOI:
10.1093/jee/tov338.
- Nevesa, R.C.S., Torresa, J.B. & Zanunciob, J.C. (2010).
Production and storage of mealworm beetle as prey
for predatory stinkbug. Biocontrol Science and
Technology, 20(10), 1013-1025. DOI:
10.1080/09583157.2010.500718.
- Ochieng-Odero, J.P.R. (1992). The effect of three constant
temperatures on larval critical weight, latent feding
period, larval maximal weight and fecundity of
Cnephasia jactatana (Walker) (Lepidoptera:
Tortricidae). Journal of Insect Physiology, 38(2),
127-130. DOI: 10.1016/0022-1910(92)90041-B.
- Oonincx, D.G.A.B., Van Broekhoven, S., Van Huis, A. &
Van Loon J.J.A. (2015). Feed conversion, survival
and development, and composition of four insect
species on diets composed of food by-products. Plos
One, 10(12), 1-20. DOI:
10.1371/journal.pone.0144601.
- Ravzanaadii, N., Kim, S., Choi, W.H., Hong, S. & Kim,
N.J. (2012). Nutritional value of mealworm,
Tenebrio molitor as food source. International
Journal of Industrial Entomology, 25(1), 93-98.
DOI: 10.7852/ijie.2012.25.1.093.
- Sasmita, H.I., Tu, W., Bong, L. & Neoh, K. (2019). Effects
of larval diets and temperature regimes on life
history traits, energy reserves and temperature
tolerance of male Aedes aegypti (Diptera:
Culicidae): optimizing rearing techniques for the
sterile insect programmes. Vectors, 12, 2-16. DOI:
10.1186/s13071-019-3830-z.
- Sinclair, B.J. & Marshall, K.E. (2018). The many roles of
fats in overwintering insects. Journal of
Experimental Biology, 7(221), 1-9. DOI:
10.1242/jeb.161836.
- Sørensen, J.G., Addison M.F. & Terblanche J.S. (2012).
Mass-rearing of insects for pest management:
Challenges, synergies and advancesfrom
evolutionary physiology. Crop Protection, 38, 87-
94. DOI: 10.1016/j.cropro.2012.03.023.
- Sönmez, E. (2021). The effect of different cold storage period
on total lipid amount of Tenebrio molitor
(Coleoptera: Tenebrionidae) larvae. Journal of
Anatolian Environmental and Animal Sciences, 6(3),
449-455. DOI: 10.35229/jaes.970307.
- Stanley-Samuelson, D.W., Jurenka, R.A., Cripps, C.,
Blomquist, G.J. & Renobales, M. (1988). Fatty
acids in insects: composition, metabolism, and
biological significance. Archive of Insect
Biochemistry and Physiology, 9(1), 1-33. DOI:
10.1002/arch.940090102.
- Van Broekhoven, S., Oonincx, D.G., Van Huis, A. & Van
Loon, J.J. (2015). Growth performance and feed
conversion efficiency of three edible mealworm
species (Coleoptera: Tenebrionidae) on diets
composed of organic by-products. Journal of Insect
Physiology, 73, 1-10. DOI:
10.1016/j.jinsphys.2014.12.005.
- Via, S. (1999). Cannibalism facilitates the use of a novel
environment in the flour beetle, Tribolium
castaneum. Heredity, 82(3), 267-275. DOI:
10.1038/sj.hdy.6884820.
- Weaver, D.K. & McFarlane, J.E. (1990). The effect of larval
density on growth and development of Tenebrio
molitor. Journal of Insect Physiology, 36(7), 531-
536. DOI: 10.1016/0022-1910(90)90105-O.
- Zaelor, J. & Kitthawee, S. (2018). Growth response to
population density in larval stage of darkling beetles
(Coleoptera; Tenebrionidae) Tenebrio molitor and
Zophobas atratus. Agriculture and Natural
Resources, 52(6), 603-606. DOI:
10.1016/j.anres.2018.11.004.