Maya Kaynaklı Sinbiyotik (JD2+EPSJD2) ve Postbiyotik+Prebiyotik (CFSJD2+EPSJD2) Uygulamalarının Bazı Biyolojik Aktivitelerinin Belirlenmesi
Ekzopolisakkaritin (EPS) biyolojik potansiyeli, probiyotik mikroorganizmaların yetiştirilmesinde kullanılan fermantasyon koşullarından etkilenen kimyasal yapısına bağlıdır. Probiyotik maya tarafından üretilen EPS, antimikrobiyal, immünomodülatör, anti-inflamatuar, antioksidan, anti-tümör, anti-viral, anti-diyabetik, anti-ülser ve kolesterol düşürücü aktiviteler gibi terapötik uygulamalarda çok önem kazanmıştır. Bu çalışmada, Pichia kudriavzevii mayası kullanılarak elde edilen sinbiyotik (JD2+EPSJD2), postbiyotik+prebiyotik (CFSJD2+EPSJD2) uygulamalarının bazı biyolojik aktivite (antioksidan ve antibiyofilm) çalışmalarının yapılması amaçlanmıştır. Elde edilen sonuçların etkili/etkisiz olduğunu yorumlayabilmek için aynı koşullarda ticari prebiyotik olarak satılan inülin kullanılmış ve analiz sonuçları karşılaştırılmıştır. Farklı konsantrasyonlar denenerek belirlenen biyolojik aktivite çalışmalarında 10 mg/L derişimde sırasıyla en yüksek antioksidan ve antibiyofilm kapasite postbiyotik+prebiyotik (CFSJD2+EPSJD2) (%86,6 ve %84, sırasıyla) uygulamasında tespit edilmiştir. Ayrıca, ticari prebiyotik olarak kullanılan inülinin antioksidan aktivitesinin (%71,4) ve biyofilm oluşumunu engelleme (%68) kapasitesinin araştırmamızda kullandığımız uygulamalardan daha düşük değerde olduğu gözlenmiştir.
Determination of Some Biological Activities of Yeast Derived Synbiotic (JD2+EPSJD2) and Postbiotic+Prebiotic (CFSJD2+EPSJD2) Applications
The biological potential of exopolysaccharide (EPS) depends on its chemical structure, which is affected by the fermentation conditions used in the cultivation of probiotic microorganisms. EPS produced by probiotic yeast has gained great importance in therapeutic applications such as antimicrobial, immunomodulatory, anti-inflammatory, antioxidant, anti-tumor, anti-viral, anti-diabetic, anti-ulcer, and cholesterol-lowering activities. In this study, it was aimed to conduct some biological activity (antioxidant and antibiofilm) studies of synbiotic (JD2+EPSJD2), postbiotic+prebiotic (CFSJD2+EPSJD2) applications obtained by using Pichia kudriavzevii yeast. To interpret the results obtained as effective/ineffective, commercial prebiotic inulin was used under the same conditions and the analysis results were compared. In biological activity studies determined by testing different concentrations, the highest antioxidant and antibiofilm capacity was identified in postbiotic+prebiotic (CFSJD2+EPSJD2) (86.6% and 84%, respectively) application, respectively, at 10 mg/L concentration. In addition, it was observed that the antioxidant activity (71.4%) and the capacity to prevent biofilm formation (68%)of inulin, which is used as a commercial prebiotic, were lower than the applications we used in our research.
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- Aakef, J.N.A. (2018). Hurmadan izole edilen mayaların bazı
probiyotik özelliklerin araştırılması. Yüksek Lisans
Tezi, Gazi Üniversitesi Fen Bilimleri Ensititüsü,
Ankara. 118.
- Aguilar-Toala, J.E, Hall, F.G., Urbizo-Reyes, U.C., Garcia, H.S., Vallejo-Cordoba, B., González-
Córdova, A.F., Hernández-Mendoza, A. & Liceaga, A.M. (2019). In silico prediction & in vitro
assessment of multifunctional properties of postbiotics obtained from two probiotic bacteria.
Probiotics & Antimicrobial Proteins, 12(2), 608-
622. DOI: 10.1007/s12602-019-09568-z.
- Al-Shwyeh, A. (2019). Date palm (Phoenix dactylifera L.)
fruit as potential antioxidant & antimicrobial agents.
Journal of Pharmacy & Bioallied Sciences, 11(1), 1-
11. DOI: 10.4103/jpbs.JPBS_168_18.
- Amaretti, A., Di Nunzio, M., Pompei, A., Raimondi, S.,
Rossi, M. & Bordoni, A. (2013). Antioxidant
properties of potentially probiotic bacteria: In vitro
& in vivo activities. Applied Microbial
Biotechnology, 97(2), 809-817. DOI:
10.1007/s00253-012-4241-7.
- Andresen, V., Gschossmann, J. & Layer, P. (2020). Heat-
inactivated Bifidobacterium bifidum MIMBb75 (SYN-HI-001) in the treatment of irritable bowel syndrome: a multicentre, r&omised, double-blind, placebo-controlled clinical trial. Lancet Gastroenterology Hepatology, 5(7), 658-666. DOI:10.1016/S2468-1253(20)30056-X.
- Aponte, M., Murru. N. & Shoukat, M. (2020). Therapeutic, prophylactic, & functional use of probiotics: A current perspective. Frontiers in Microbiology, 11(562048), 1-16. DOI:10.3389/fmicb.2020.562048.
- Ayaz, Z. (2021). Prebiyotikler ve sağlık açısından faydaları. The Journal of Turkish Family Physician, 12(4),
201-206. DOI: 10.15511/tjtfp.21.00493 Bajaj, B. K., Raina, S. & Signh, S. (2013). Killer toxin from a novel killer yeast Pichia kudriavzevii RY55 with idiosyncratic antibacterial activity. Journal of Basic Microbiology, 53, 645-656. DOI:10.1002/jobm.201200187.
- Banik, A., Halder, S., Ghosh, C. & Mondal, C. (2019). Fungal probiotics: Opportunity, challenge, & prospects. Recent Advancement in White Biotechnology through Fungi, 11235, 101-117. DOI: 10.1007/978-3-030-14846-1_3.
- Banwo, K., Alonge, Z. & Sanni, A.I. (2021). Binding capacities & antioxidant activities of Lactobacillus plantarum & Pichia kudriavzevii against cadmium & lead toxicities. Biological Trace Element Research, 199(2), 779-791. DOI: 10.1007/s12011-020-02164-1.
- Bekatorou, A., Psarianos, C. & Koutinas, A.A. (2006). Production of food grade yeast. Biotechnology, 44(3), 407-415. ISSN: 1330-9862.
- Bikric, S., Aslim, B., Dincer, İ., Yuksekdag, Z., Ulusoy, S. & Yavuz, S. (2022). Characterization of exopolysaccharides (EPSs) obtained from Ligilactobacillus salivarius strains & investigation
at the prebiotic potential as an alternative to plant prebiotics at poultry. Probiotics & Antimicrobial
Proteins, 14, 49-59. DOI: 10.1007/s12602-021-09790-8.
- Borman, A.M. & Johnson, E.M. (2021). New names for
fungi of medical importance: Can we have our cake
& eat it too? Journal of Clinical Microbiology,
59(3). DOI: 10.1128/JCM.02896-20.
- Burkhardt, L. (2022). Eine enzyklopädie zu eponymischen
pflanzennamen [Encyclopedia of eponymic plant
names] (in German). Berlin: Botanic Garden &
Botanical Museum, Freie Universität Berlin.
- Castro-Bravo, N., Wells, J.M., Margolles, A. & Ruas-
Madiedo, P. (2018). Interactions of surface exopolysaccharides from Bifidobacterium & Lactobacillus within the intestinal environment. Frontiers in Microbiology, 9, 2426. DOI:/10.3389/fmicb.2018.02426.
- Chaieb, K., Kouidhi, B., Jrah, H., Mahdouani, K. &
Bakhrouf, A. (2011). Antibacterial activity of
thymoquinone, an active principle of nigella sativa
& its potency to prevent bacterial biofilm formation.
BMC Complementary & Alternative Medicine, 11
29. http://www.biomedcentral.com/1472-
6882/11/29.
- Çiftçi, M. & Öncül, N. (2022). Ticari probiyotik içeceklerin
bazı mikrobiyolojik özellikleri. Akademik Ziraat
Dergisi, 11(1), 165-178. DOI:
10.29278/azd.1002242.
- Das, D., Baruah, R. & Goyal, A. (2014). A food additive
with prebiotic properties of an alpha-d-glucan from
Lactobacillus plantarum DM5. International
Journal of Biological Macromolecules, 69, 20-26.
DOI: 10.1016/j.ijbiomac.2014.05.029.
- Datta, S., Timson, D.J. & Annapure, U.S. (2017).
Antioxidant properties & global metabolite
screening of the probiotic yeast Saccharomyces
cerevisiae var. boulardii. Journal of the Science of
Food & Agriculture, 97(9), 3039-3049. DOI:
10.1002/jsfa.8147.
- de Oliveira Coelho, B., Fiorda-Mello, F., de Melo Pereira,
G., Thomaz-Soccol, V., Rakshit, S.K., de
Carvalho, J.C. & Soccol1, C.R. (2019). In vitro
probiotic properties & DNA protection activity of yeast & lactic acid bacteria isolated from a honey-
based kefir beverage. Foods, 8(10), 485. DOI:10.3390/foods8100485.
- Doğan, M. (2012). The effect mechanisms of probiotic
bacteria in gastrointestinal system. Electronic Journal of Food Technologies, 7(1), 20-27. e-
ISSN:1306-7648.
- Douglass A.P., Offei B., Galleani, S.B., Coughlan, A.Y.,
Martos, A.A.R., Ortiz-Merino, R.A., Byrne K.P.
& Wolfe, K.H. (2018). Population genomics shows
no distinction between pathogenic C&ida krusei &
environmental Pichia kudriavzevii: One species,
four names. PLoS Pathogens, 14(7), e1007138.
DOI: 10.1371/journal.ppat.1007138.
- El-Ghawas, D.E., Elkhateeb, W.A, Akram, M. & Daba,
G.M. (2021). Yeast as biotechnological tool in food industry. Journal of Pharmaceutical Sciences, 5(2),
1-6.
- Elleuch, M., Besbes, S., Roiseux, O., Blecker, C.,
Deroanne, C., Drira, N. &Attia, H. (2008). Date
flesh: Chemical composition & characteristics of the
dietary fibre. Food Chemistry, 111(3), 676-682.
DOI: 10.1016/j.foodchem.2008.04.036.
- Fiore, C., Arrizon, J., Gschaedler A., Flores, J. & Romano,
P. (2005). Comparison between yeasts from grape &
agave musts for traits of technological interest.
World Journal Microbiology Biotechnology, 21,
1141-1147. DOI: 10.1007/s11274-005-0196-5.
- Gibson, G., Hutkins, R., Sanders, M., Prescott, S., Reimer,
R. & Salminen, S. (2017). Expert consensus
document: The International Scientific Association
for Probiotics and Prebiotics (ISAPP) consensus
statement on the definition & scope of prebiotics
(PDF). Nature Reviews. Gastroenterology &
Hepatology, 14(8), 491-502. DOI:
10.1038/nrgastro.2017.75.
- Gientka, I., Blazejak, S., Stasiak-Rozanska, L. &
Chlebowska-Smigiel, A. (2015).
Exopolysaccharides from yeast: Insight into optimal
conditions for biosynthesis, chemical composition &
functional properties. Acta Scientiarum Polonorum
Technologia Alimentaria. 14(4), 283-292. DOI:
10.17306/J.AFS.2015.4.29.
- Hill, C., Guarner, F., Reid, G., Gibson, G.R., Merenstein,
D.J., Pot, B., Morelli, L., Canani, R.B., Flint, H.J.,
Salminen, S., Calder, P.C. & Sanders, M.E.
(2014). Expert consensus document: the
international scientific association for probiotics &
prebiotics consensus statement on the scope &
appropriate use of the term probiotic. Nature
Reviews Gastroenterology & Hepatology, 11(8),
506-514.
- Homayouni, A., Azizi, A., Oroojzadeh, P. & Pourjafar, H.
(2020). Kluyveromyces marxianus as a probiotic
yeast: A mini-review. Current Nutrition & Food
Science, 16(8), 1163-1169. DOI:
10.2174/1573401316666200217113230.
- Humam, A.M., Loh, T.C., Foo, H.L., Izuddin, W.I.,
Zulkifli, I., Samsudin, A.A. & Mustapha, N.M.
(2021). Supplementation of postbiotic RI11
improves antioxidant enzyme activity, upregulated
gut barrier genes, & reduced cytokine, acute phase
protein, & heat shock protein 70 gene expression
levels in heat-stressed broilers. Poultry Science,
100(3), 100908. DOI: 10.1016/j.psj.2020.12.011.
- Illanes, A. & Guerrero, C. (2016). Functional foods & feeds:Probiotics, prebiotics, & synbiotics. Lactose-
Derived Prebiotics. A Process Perspective, 35-86. DOI: 10.1016/B978-0-12-802724-0.00002-0.
- Kanmani, R., Dhivya, S., Jayalakshmi, S. & Vijayabaskar,
P. (2011). Studies on detergent additives of protease
enzyme from an estuarine bacterium Bacillus cereus. International Research Journal of Biotechnology,
27, 157-163.
- Karaca, B., Haliscelik, O., Gursoy, M., Kiran, F.,
Loimaranta, V., Söderling, E. & Gursoy, U.K.
(2022). Analysis of chemical structure & antibiofilm
properties of exopolysaccharides from
Lactiplantibacillus plantarum EIR/IF-1. Postbiotics.
Microorganisms, 10, 2200. DOI:
10.3390/microorganisms10112200.
- Kerry, R.G., Patra, J.K., Gouda, S., Park, Y., Shin, H.S.,
Das, G. (2018). Benefaction of probiotics for human
health: A review. Journal of Food & Drug Analysis,
26(3), 927-939. DOI: 10.1016/j.jfda.2018.01.002.
- Kim, Y., Oh, S. & Kim, S.H. (2009). Released
exopolysaccharide (r-EPS) produced from probiotic
bacteria reduce biofilm formation of
enterohemorrhagic Escherichia coli O157:H7.
Biochemical & Biophysical Research
Communications, 379(2), 324-329. DOI:
10.1016/j.bbrc.2008.12.053.
- Koohestani, M., Moradi, M., Tajik, H. & Badali, A. (2018).
Effects of cell-free supernatant of Lactobacillus
acidophilus LA5 & Lactobacillus casei 431 against
planktonic form & biofilm of Staphylococcus
aureus. Veterinary Research Forum, 9(4), 301-306.
DOI: 10.30466/vrf.2018.33086.
- Li, W., Ji, J., Chen, X., Jiang, M., Rui, X. & Dong, M.
(2014). Structural elucidation & antioxidant
activities of exopolysaccharides from Lactobacillus
helveticus MB2-1. Carbohydrate Polymers, 102,
351-359. DOI: 10.1016/j.carbpol.2013.11.053.
- Li, S., Huang, R.., Shah, N., Tao, X., Xiong, Y. & Wei, H.
(2014). Antioxidant and antibacterial activities of
exopolysaccharides from Bifidobacterium bifidum
WBIN03 and Lactobacillus plantarum R315.
Journal of Dairy Science, 97(12), 7334 -7343.
- Madigan, M., Bender, K., Buckley, D., Sattley, M. & Stahl,
D. (2019). Brock Biology of Microorganisms, 15th
Global Edition, 1064s.
- Merchán, A.V., Benito, M.J., Galván, A.I. & Ruiz-Moyano
Seco de Herrera, S. (2020). Identification &
selection of yeast with functional properties for
future application in soft paste cheese. Lebensmittel-
Wissenschaft & Technologie (LWT), 124, 109173. DOI: 10.1016/j.lwt.2020.109173.
- Moslehi-Jenebian, S., Pedersen, L.L. & Jespersen, L.
(2010). Beneficial effects of probiotic & food borne
yeasts on human health. Nutrients, 2(4), 449-73.
DOI: 10.3390/nu2040449.
- Nahar, S., Mizan, F.R., Ha, A.J.W. & Ha, S.D. (2018).
Advances & future prospects of enzyme-based
biofilm prevention approaches in the food industry.
Comprehensive Reviews in Food Science & Food
Safety, 17(6), 1484-1502. DOI: 10.1111/1541-
4337.12382.
- Oberoi, H.S, Babbar, N., Sandhu, S.K., Dhaliwal, S.S.,
Kaur, U., Chadha, B.S. & Bhargav, V.K. (2012).
Ethanol production from alkali-treated rice straw via
simultaneous saccharification & fermentation using
newly isolated thermotolerant Pichia kudriavzevii
HOP-1. Journal of Industrial Microbiology &
Biotechnology, 39(4), 557-556. DOI:
10.1007/s10295-011-1060-2.
- Okan Bakır, B. (2011). Prebiyotik, probiyotik ve
sinbiyotiklere genel bakış. Beslenme ve Diyet
Dergisi, 40(2), 178-182.
- Pandey, K.R., Naik, S.R. &Vakil, B.V. (2015). Probiotics,
prebiotics & synbiotics- a review. Journal of Food
Science & Technology, 52(12), 7577-7587. DOI:
10.1007/s13197-015-1921-1.
- Rashad, M.M., Mahmoud, A.E., Abdou, H.M. & Nooman,
M.U. (2011). Improvment of nutritional quality of
yeast antioxidant activities of yeast fermented
soybean curd resides. African Journal of
Biotechnology, 10(28), 5504-5513. DOI:
10.5897/AJB10.1658.
- Rengel dos Passos, F., Maestre, K.L., Florêncio da Silva,
B., Rodrigues, A.C., Triques, C. C., Garcia, H.A.,
Fagundes-Klen, M.R., Antonio da Silva, E. &
Fiorese, M.L. (2021). Production of a synbiotic
composed of galacto-oligosaccharides &Saccharomyces boulardii using enzymatic-
fermentative method. Food Chemistry, 353, 129486. DOI: 10.1016/j.foodchem.2021.129486.
- Sadeghi, A., Ebrahimi, M., Shahryari, S., Kharazmi, M. &
Jafari, S. (2022). Food applications of probiotic
yeasts; focusing on their techno-functional,
postbiotic and protective capabilities. Trends in
Food Science and Technology, 128, 278-295.
- Salminen, S., Collado, M. C., Endo, A., Hill, C., Lebeer, S.,
Quigley, E. M. M. , Sanders, M. E., Shamir, R.,
Swann, J. R., Szajewska, H. & Vinderola, G.
(2021). The International Scientific Association of
Probiotics & Prebiotics (ISAPP) consensus
statement on the definition & scope of postbiotics.
Nature Reviews Gastroenterology & Hepatology,
18, 649-667. DOI: 10.1038/s41575-021-00440-6.
- Sandasi, M., Leonard, C.M. & Viljoen, A.M. (2010). The in
vitro antibiofilm activity of selected culinary herbs
& medicinal plants against Listeria monocytogenes.
Letters in Applied Microbiology, 50(1), 30-35. DOI:
10.1111/j.1472-765X.2009.02747.x.
- Sarikaya, H., Aslim, B. & Yuksekdag, Z.N. (2017).
Assessment of anti-biofilm activity & bifidogenic
growth stimulator (BGS) effect of lyophilized
exopolysaccharides (L-EPSs) from Lactobacilli
strains. International Journal of Food Properties,
20(2), 362-371. DOI:
10.1080/10942912.2016.1160923.
- Shi, Y., Davis, K., Zhang, F., Duffy, C & Yu, X. (2014).
Parameter estimation of a physically-based l&surface hydrologic model using the ensemble
Kalman Filter: A synthetic experiment. Water
Resources Research, 50, 706-724. DOI:
10.1002/2013WR014070.
- Smith, I.M., Baker, A., Arneborg, N. & Jespersen, L.
(2015). Non Saccharomyces yeasts protect against
epithelial cell barrier disruption induced by
Salmonella enterica subsp. enterica serovar
Typhimurium. Applied Microbiology, 161(5), 491-7.
DOI: 10.1111/lam.12481.
- Soccol, C.R., Vandenberghe, L.P.S., Spier, M.R.,
Medeiros, A.B.P., Yamaguishi, C.T., Lindner,
J.D.D., P&ey, A. & Thomaz-Soccol, V. (2010).
The potential of probiotics. Food Technology &
Biotechnology, 48(4), 413-434. ISSN 1330-9862.
- Soliemani, O., Salimi, F. & Rezaei, A. (2022).
Characterization of exopolysaccharide produced by
probiotic Enterococcus durans DU1 & evaluation of
its anti-biofilm activity. Archives of Microbiology,
Archives of Microbiology, 204, 419. DOI:
10.1007/s00203-022-02965-z.
- Stinson, L.F., Payne, M.S. & Keelan, J.A. (2017). Planting
the seed: origins, composition, & postnatal health
significance of the fetal gastrointestinal microbiota.
Critical Reviews in Microbiology, 43(3), 352-369.
DOI: 10.1080/1040841X.2016.1211088.
- Swanson, K.S., Gibson, G.R., Hutkins, R., Reimer, R.A.,
Reid, G., Verbeke, K., Scott, K.P., Holscher, H.D.,
Azad, M.B., Delzenne, N.M. & Sanders, M.E.
(2020). The International Scientific Association for
Probiotics & Prebiotics (ISAPP) consensus
statement on the definition & scope of synbiotics.
Nature Reviews Gastroenterology & Hepatology,
17, 687-701. DOI: 10.1038/s41575-020-0344-2.
- Toushik, S.H., Mizan, F.R., Hossain, I. & Ha, S.D. (2020). Fighting with old foes: The pledge of microbe-
derived biological agents to defeat mono-& mixed-bacterial biofilms concerning food industries. Trends in Food Science & Technology, 99, 413-425. DOI: 10.1016/j.tifs.2020.03.019.
- Tufarelli, V. & Laudadio, V. (2016). An Overview on the
functional food concept: prospectives & applied
researches in probiotics, prebiotics & synbiotics.
Journal of Experimental Biology & Agricultural
Sciences. 4(3), 273-278. ISSN: 2320-8694.
- Vargas-Ochoa, B., Mejía-Barajas, J., Clemente-Guerrero,
M., Manzo-Avalos, S., Salgado-Garciglia, R. &
Saavedra-Molina, A. (2016). Evaluation of
antioxidant activity from different yeast extracts.
Experimental Biology, 30(S1), 1174.22-1174.22.
DOI: 10.1096/fasebj.30.1_supplement.1174.22.
- Wang, J., Zhao, X., Yang, Y., Zhao, A. & Yang, Z. (2015).
Characterization & bioactivities of an
exopolysaccharide produced by Lactobacillus
plantarum YW32. International Journal of Biological Macromolecules. 74, 119-126. DOI:
10.1016/j.ijbiomac.2014.12.006.
- Wang, Z., Zhao, Y., Jiang, Y. & Chu, W. (2021). Prebiotic,
antioxidant, and immunomodulatory properties of
acidic exopolysaccharide from marine Rhodotorula
RY1801. Frontiers in Nutrition, 8, 134-141. DOI:
10.3389/fnut.2021.710668.
- Xu, X., Peng, Q., Zhang, Y., Tian, D., Zhang, P., Huang,
Y., Ma, L., Dia, V. P., Qiao, Y. & Shi, B. (2020).
Antibacterial potential of a novel Lactobacillus casei
strain isolated from Chinese northeast sauerkraut &
the antibiofilm activity of its exopolysaccharides.
Food & Function, 11, 4697-4706. DOI:
10.1039/D0FO00905A.
- Yang, X., Li, L., Duan, Y. & Yang, X. (2017). Antioxidant
activity of JM113 in vitro & its protective effect on
broiler chickens challenged with deoxynivalenol. J.
Anim. Sci., 95(2), 837-846. DOI:
10.2527/jas.2016.0789.
- Yi, Y., Huang, W. & Ge, Y. (2008). Exopolysaccharide: A
novel important factor in the microbial dissolution of
tricalcium phosphate. World Journal of
Microbiology & Biotechnology, 24(7), 1061. DOI:
10.1007/s11274-007-9575-4.