Siyah Çay ve Siyah Çay Atığı Örneklerinin Farklı Çözgen konsantrasyonlarında Elde Edilen Ekstraktlarının Biyoaktif Özellikleri ve Antimikrobiyel Aktivitelerinin Belirlenmesi

Bu çalışma siyah çay atıklarının antioksidan ve antimikrobiyal fenolik bileşik kaynağı olarak katma değerli bir ürün potansiyeli olup olamayacağının belirlemek amacıyla gerçekleştirilmiştir. Ekstraksiyon çözücüsünün (su, %50, %80 ve %96 etanol) siyah çay ve siyah çay atığı örnekleri üzerine etkileri ve bunların toplam fenolik, antioksidan özellik (DDPH ve ABTS) ve antimikrobiyal aktivite üzerindeki etkileşimi incelenmiştir. Siyah çay ve Siyah çay atığı örneklerinin toplam fenolik madde içerikleri sırasıyla 48,79–82,61 ve 42,37–74,03 mg GAE/g aralığında değiştiği saptanmıştır. Farklı etanol konsantrasyonları ile ekstrakte edilen Siyah Çay Atığı örneklerinin antioksidan özellikleri karşılaştırıldığında en yüksek DPPH radikal süpürme etkisi ve ABTS antioksidan kapasite değeri %80 etanol ile ekstrakte edilen örneklerde gerçekleştiği ve sırasıyla %41,86 ve 2,23 µmol TE/mg ekstrakt değerlerinde olduğu saptanmıştır. Genel olarak, Siyah çay atığı ekstraktlarının seçilen patojen mikroorganizmalara karşı gösterdikleri antimikrobiyel etkinlikler, ilave edilen ekstrakt miktarına bağlı olarak Siyah çay ekstraktlarına göre daha yüksek değerlere sahip olduğu belirlenmiştir. Siyah çay atığı örneklerinin gıda takviyesi, ilaç ve yenilebilir ambalaj sektörlerindeki uygulamalar için antioksidan ve antimikrobiyal özelliklere sahip katma değerli ürünlerin geliştirilmesi için hammadde olarak kullanılabilir olduğu düşünülmektedir.

Anahtar Kelimeler:

Camellia sinensis, ekstraksiyon, biyoaktivite, antimikrobiyel aktivite, Camellia sinensis., extraction, bioactivity, antimicrobial activity

Determination of Bioactive Properties and Antimicrobial Activities of Extracts of Black Tea and Black Tea Waste Samples Obtained at Different Solvent Concentrations

This study was carried out to determine whether black tea wastes could be a value-added product potential as a source of antioxidant and antimicrobial phenolic compounds. The effects of extraction solvent (water, 50%, 80% and 96% ethanol) on black tea and black tea waste samples and their interaction on total phenolic, antioxidant properties (DDPH and ABTS) and antimicrobial activity were investigated. It was determined that the total phenolic content of black tea and black tea waste samples ranged between 48.79–82.61 and 42.37–74.03 mg GAE/g, respectively. When the antioxidant properties of Black Tea Waste samples extracted with different ethanol concentrations were compared, it was determined that the highest DPPH radical scavenging effect and ABTS antioxidant capacity value were observed in the samples extracted with 80% ethanol, with 41.86% and 2.23 µmol TE/mg extract, respectively. In general, it has been determined that the antimicrobial activities of Black tea waste extracts against selected pathogenic microorganisms have higher values than Black tea extracts depending on the amount of added extract. It is thought that black tea waste samples can be used as raw materials for the development of value-added products with antioxidant and antimicrobial properties for applications in the food supplement, pharmaceutical and edible packaging industries.

Keywords:

Camellia sinensis., extraction, bioactivity, antimicrobial activity,

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