Kadir YURDAKOÇ, Aylin ALTINIŞIK TAGAÇ, Önder SARP

C Vitaminin Kitosan Nanoparçacıklardan Kontrollü Salımı

Bu çalışma iki aşamadan oluşmaktadır. Birinci aşama kitosanın nanoparçacıkların (ChNPs) sentezi ve karakterizasyonudur. İkincisi aşama, C vitamin yüklü ChNPS eldesi ve C vitamininin salım kinetiğinin incelenmesidir. ChNPs iyonik jelleşme yöntemine göre sentezlenmiştir. Sodyum tripolifosfat (TPP) çapraz bağlayıcı olarak kullanılmıştır. Sentezlenen ChNPs örneklerinin tanecik boyutu dağılımları, yüzey morfolojileri ve kristal yapıları sırasıyla Nanosizer, Taramalı Electron Mikroskobu (SEM) ve X-ışını difraktometresi (XRD) ile incelenmiştir. Hazırlanan ChNPs örneklerinin yapısal analizleri ve termal özellikleri sırasıyla, Fourier Transform Infrared (FTIR) spektrofotometresi ve termal gravimetrik analiz ile incelenmiştir. Aynı zamanda C vitamin yüklü ChNPs örneklerinin salım profilleri belirlenmiştir. Çalışmaların sonucunda, ChNPs örneklerinin ortalama parçacık boyutları 10 nm olarak ölçülmüştür. C vitamini yükleme verimi %86 C vitamini derişimi olarak hesaplanmıştır. Son olarak, C vitaminin ChNPs örneklerinden salım mekanizmasının difüzyon ve şişme kontrollü olduğu belirlenmiştir.

Controlled Release of Vitamin C from Chitosan Nanoparticles

This work is consisted of two parts. The first was the synthesis and characterization of nanoparticles (ChNPs) from Chitosan, a natural biopolymer. In the second part, preparation of Vitamin C loaded ChNPs and release of vitamin C from the loaded nanoparticles were investigated. ChNPs were synthesized according to the ionic gelation method and sodium tripolyphosphate (TPP) was used as the crosslinking agent. The particle size distribution of the synthesized ChNPs was determined by using Zeta Sizer. Surface morphologies and crystal structures of the nanoparticles were investigated by Scanning Electron Microscopy (SEM) and X- ray diffraction (XRD) analysis, respectively. Structural analysis and thermal properties of ChNPs were also investigated by Fournier Transform Infrared Spectroscopy (FTIR) and thermogravimetric analysis (TGA), respectively. Release porofile of the Vitamin C loaded nanoparticles at same time were determined. As a result, average particle size of the ChNPs was measured as 10 nm and loading efficiency of the ChNPs was calculated as 86% with very high vitamin C concentration. Finally, the release mechanism of vitamin C from nanoparticles was determined to be controlled by diffusion and swelling.

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