Bijen KIVÇAK, Tuğce Fafal ERDOĞAN, Tuba GÖNENÇ, Aysun PABUÇCUOĞLU, Esma ORAN, Ferdane KAHRAMAN, Tansel ÖZTÜRK

Erıca bocquetıı p. F. Stevens And Erıca arborea L.’ nın Antioksidan, Antimikrobiyal Ve Sitotoksik Aktiviteler

Araştırmanın amacı Erica arborea L. ve Türkiye için endemik olarak bilinen Erica bocquetii P.F. Stevens' in toprak üstü kısımlarının n-hekzan, etanol, metanol, etil asetat ve su ekstrelerinin in vitro antimikrobiyal, antioksidan ve sitotoksik aktivitelerini araştırmaktır. Antimikrobiyal aktivite disk difüzyon metodu ve Broth Tüp Dilüsyon metodunun minimum inhibitör konsantrasyonu (MIC) ile araştırılmıştır. Antioksidan aktivite [2,2/-azinobis-(3-etilbenzotiazolin-6-sülfonik asit)] (ABTS+) radikal mono katyonunun dekolorizasyonu esas alınarak yapılan bir analiz ile belirlenmiştir. Sitotoksik aktiviteler Brine shrimp biyolojik denemeleri ile gerçekleştirilmiştir. Erica türlerinin tüm ekstrelerinin bazı bakterilere karşı antimikrobiyal aktiviteye sahip olduğu bulundu, fakat mayaya karşı aktivite gözlenmedi. E. bocquetii ekstrelerinin antioksidan potansiyelleri E. arborea' nınkilerden iki kat yüksek olduğu saptandı. Erica ekstreleri Brine shrimp' e karşı sitotoksik aktivite göstermemiştir.

Anahtar Kelimeler:

Erica bocquetii, Erica arborea, Antioksidan Aktivite, Antimikrobiyal Aktivite, Sitotoksisite, Brine Shrimp.

Antioksidant, Antimicrobial And Cytotoxic Activities Of Erica bocquetıı p.f. stevens and Erica arborea L.

The aim of this study was to investigate in vitro antimicrobial, antioxidant, and cytotoxic activities of n-hexanoic, ethanolic, methanolic, ethyl acetate, and aqueous extracts of the aerial parts of Erica arborea L. and Erica bocquetii P.F. Stevens known to be endemic species for Turkey. The antimicrobial activity was investigated by disc diffusion method, and the minimal inhibitory concentration (MIC) by Broth Microdilution method. The antioxidant activity was determined by an improved assay based on the decolorization of the radical monocation of [2,2/-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)] (ABTS+). The cytotoxic activities were evaluated using Brine shrimp bioassay. The all extracts both species of Erica were also found to possess antimicrobial activity against some of the bacteria, but no activity was observed against the yeast. The antioxidant potentials of the extracts of E. bocquetii were obtained two times higher than those of E. arborea. Erica extracts did not exhibit the cytotoxic activity against Brine shrimp.

Keywords:

Erica bocquetii, Erica arborea, Antioxidant Activity, Antimicrobial Activity, Cytotoxicity, Brine Shrimp,

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Çelikel N., Kavas G. Antimicrobial properties of some essential oils against some pathogenic microorganisms. Czech Journal of Food Science 2008; 26 (3): 174-181. Asadbagi M., Karamian R., Hasanain P. Antioxidant activity of methanol extract of Salvia aethiopis L. on circulatory system of rat. Journal of Herbal Drugs 2011; 2 (3): 217-221. Gutteridge J.M.C. Biological origin of free radicals and mechanisms of antioxidant protection. Chemico-Biological Interactions 1994; 91: 133-140. Steinmetz K.A., Potter J.D. Vegetables, fruit, and cancer prevention: A review. Journal of American Dietetic Association 1996; 96: 1027-1039. Aruoma O.L. Free radicals, oxidative stress, and antioxidants in human health and disease. Journal of American Oil Chemists Society 1998; 75: 199-212. Pieroni A., Janiak V., Dürr C.M., Lüdeke S., Trachsel E., Heincrich M. In vitro antioxidant activity of non-cultived vegetables of ethnic Albanians in Southern Italy. Phytotheraphy Research 2002; 16: 467-473. Couladis M., Tzakou O., Verykokidou E., Harvala C. Screening of some Greek aromatic plants for antioxidant activity. Phytotheraphy Research 2003; 17: 194-195. WHO World Health Report Office of World Health Reporting. Geneva, Switzerland19
Davies J. Inactivation of antibiotics and the dissemination of resistance genes. Science 1994; 264: 375-382. Nikaido H. Prevention of drug access to bacterial targets: permeability barriers and active efflux. Science 1994; 264: 382-388. Spratt B.G. Resistance to antibiotics mediated by target alterations. Science 1994; 264: 388-393. Larsen I.K. Anticancer agents. In: Krogsgaard P, Liljefors T, Madsen U, eds., A Textbook of Drug Design and Development, Harwood Academic Publisher, The Netherlands19 pp: 460-507. Yue-Zhong S. Recent naturel products based drug development: A pharmaceutical industry perspective. Journal of Natural Products 1998; 61: 1053-1071. Davis P.H. Flora of Turkey and the East Aegean Islands, Edinburg University Press, Edinburgh-1988, pp: 285-290. Baytop T. Therapy With medicinal plants in Turkey (past and present) (1st edn), Istanbul University Press, Istanbul- 1984, pp: 233. Tuzlacı E., Eryaşar Aymaz P. Turkish folk medicinal plants, Part IV: Gönen (Balıkesir). Fitoterapia 2001; 72: 323. Darias V., Martin-Herrera D., Abdala S., Fuente D. Plants used in urinary pathologies in the Canary Islands. Pharmaceutical Biology 2001; 39: 170-180. Rios J.L., Recio M.C., Villar A. Antimicrobial activity of selected plants employed in the Spanish Mediterranean area. Journal of Ethnopharmacology 1987; 21: 139-152. Harborn J.B., Williams C.A. Chemotaxonomic survey of flavonoids and simple phenols in leaves of the Ericaceae. Botaical Journal of the Linnean Society 1973; 66: 37-54. Alonso J.L:, De Landa F., Vieitez, E. Phenolic germination inhibitors from Erica arborae. Oecologica 1976; 35: 367-375. Hassan T.Y., Zorina A.D., Matyukhina L.G., Saltykova I.A. Triterpenoids of some plants growing in Syria. Russian Chemical Bulletin 1977; 1: 119-120. Akhtardzhiev K. Presence of arbutin and tannins in native representatives of family Ericaceae. Pharmazie 1996; 21: 59-60. Frutos P., Hervas G., Ramos G., Giraldez F.J., Mantecon A.R. Condensed tanin content of several shrub species from a mountain area in northern Spain and its relationship to various indicators of nutritive value. Animal Feed Science Technology 2002; 95: 215-226.
Cremer A. Antibiotic sensitivity and assay tests (5th ed). In: Collins CH, Lyne PM, eds., Microbiological methods, Butterworth & Co. (publishers) Ltd., London- 1985, pp. 1731 Seeley H.W., Vandemark P.J. Sensitivity Discs In The Therapeutic Use Of Antibiotics. Microbes In Action, A Laboratory Manual Of Microbiology (2nd ed). WH. Freeman and Company, San Francisco- 1972. NCCLS Performance Standards for Antimicrobial Disc Susceptibility Test, 6th edn. Approved Standard M2-A6, USA. National Committee for Clinical Laboratory Standards, 1997, Wayne, USA. Zgoda J.R., Porter J.R. A convenient microdilution method for screening natural products against bacteria and fungi. Pharmaceutical Biology 2001; 39: 221-225. Maclaughlin J.L., Rogers L.L., Anderson J.E. The use of biological assays to evaluate botanicals. Drug Information Journal 1994; 17: 1-13. Jimnez O.F.A., Molina J.G.A., Mendoza P.N., Leon C.F., Flores P.B, Santos S.E., Mandoki I.J. Cytostatic activity of coumarin metabolites and dervatives in the B-16-F-10 murine melanoma cell line. Melanoma Research 1999; 9: 243-247. Lee K.H. Novel antitumor agents from higher plants. Medicinal Research Reviews 1999; 19: 569-596. Re R., Pellegrini N., Proteggente A., Pannala A., Yang M., Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology&Medicine 1999; 26: 1231-1237. Trease C.E., Evans U.C. Textbook of Pharmacognosy (13nd edn), Bailliere Tindall, London- 1984. Harborn, J.B. Phtochemical Methods (2nd edn), Chapman and Hall, London- 1991. Güvenç A., Kendir G., Yıldız S. Antimicrobial activity of Erica species of Turkey. In recent Progres in Medical Plants. Vol. 20. Phytopharmacology and Therapeutic Values II. Govil, J.N., Singh, V.K., Mıshra, S.K. (eds.) Studium Press, U.S.A.- 2008, pp. 173-180. Rogger Y.S., John L.I., Mark L.W. General Microbiology, 5th ed., Macmillan education Ltd, London- 1990, pp. 626-642. Ay M., Bahadori F., Öztürk M., Kolak U., Topçu G. Antioxidant activity of Erica arborea. Fitoterapia 2007; 78: 571-573.
Fejes S., Blajovics A., Lugasi A., Lemberkovics E., Petri G., Kery A. In vitro antioxidant activity of Anthriscus cerefolium L. (Hoffm.) extracts. Journal of Ethnopharmacology 2000; 69: 259-265.