LIQUID CHROMATOGRAPHIC DETERMINATION OF SYNTHETIC ANTIOXIDANT CONTENT OF SOME VEGETABLE OILS PRODUCED IN TURKEY

Bu çalıflmada, Türkiye'de üretilen bazı bitkisel sıvı yağların sentetik antioksidan içerikleri ultraviyoledetektörlü sıvı kromatografisi ile belirlenmifltir. Butil hidroksi anisol, butil hidroksi toluen ve tert-butilhidrokinon seçilen antioksidanlardır. Kromatografik analiz öncesinde yağ örneklerindeki antioksidanlarınekstraksiyonu vorteks-karıfltırma destekli sıvı-sıvı mikroekstraksiyon tekniği ile yapılmıfltır. Ekstraksiyonçözücüsünün türü, çözücü hacmi ve ekstraksiyon süresi gibi ekstraksiyon verimini etkileyen bazıparametreler optimize edilmifltir. Optimize edilen koflullarda antioksidanlar için metodun algılama vebelirleme sınırlarının sırasıyla 0.12-0.28 µg/g ve 0.40-0.92 µg/g aralığında olduğu tespit edilmifltir. Güniçi ve günler arası kesinlik, bağıl standart sapma olarak hesaplanmıfl ve sırasıyla % 6.5 ve % 8.4'den dahadüflük bulunmufltur. Türkiye'de üretilen toplam 16 bitkisel yağ örneği sentetik antioksidanların varlığınıtespit etmek için analiz edilmifl ve örneklerdeki antioksidanların konsantrasyonlarının Türk GıdaKodeksi Yönetmeliğinde tanımlanan maksimum izin verilen seviyelerin altında kaldığı bulunmufltur

TÜRKİYE’DE ÜRETİLEN BAZI BİTKİSEL SIVI YAĞLARIN SENTETİK ANTİOKSİDAN İÇERİKLERİNİN SIVI KROMATOGRAFİSİ İLE BELİRLENMESİ

In this work, the synthetic antioxidant contents of some vegetable oils produced in Turkey were determinedby using liquid chromatography with ultraviolet detector. Butylated hydroxy anisole, butylatedhydroxy toluene, and tert-butyl hydroquinone were the synthetic antioxidants selected. The extractionof antioxidants from oil samples was carried out with vortex-assisted liquid-liquid microextraction priorto chromatographic analysis. Some parameters that affect the extraction efficiency, such as the type ofextraction solvent, the volume of solvent, and extraction time were optimized. Under the optimizedconditions, the limits of detection and limits of quantification for the antioxidants were in the ranges0.12-0.28 µg/g and 0.40-0.92 µg/g, respectively. The intra-day and inter-days precisions were calculatedin terms of relative standard deviation and found to be less than 6.5% and 8.4%, respectively. Totally, 16vegetable oil samples produced in Turkey were analyzed to detect the presence of the syntheticantioxidants and the concentrations of antioxidants in samples were found below the maximumpermitted levels defined in Turkish Food Codex Regulation

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