TÜRKİYE’DE ÜRETİLEN BAZI BİTKİSEL SIVI YAĞLARIN SENTETİK ANTİOKSİDAN İÇERİKLERİNİN SIVI KROMATOGRAFİSİ İLE BELİRLENMESİ

Bu çalışmada, Türkiye’de üretilen bazı bitkisel sıvı yağların sentetik antioksidan içerikleri ultraviyole detektörlü sıvı kromatografisi ile belirlenmiştir. Butil hidroksi anisol, butil hidroksi toluen ve tert-butil hidrokinon seçilen antioksidanlardır. Kromatografik analiz öncesinde yağ örneklerindeki antioksidanların ekstraksiyonu vorteks-karıştırma destekli sıvı-sıvı mikroekstraksiyon tekniği ile yapılmıştır. Ekstraksiyon çözücüsünün türü, çözücü hacmi ve ekstraksiyon süresi gibi ekstraksiyon verimini etkileyen bazı parametreler optimize edilmiştir. Optimize edilen koşullarda antioksidanlar için metodun algılama ve belirleme sınırlarının sırasıyla 0.12-0.28 µg/g ve 0.40-0.92 µg/g aralığında olduğu tespit edilmiştir. Gün içi ve günler arası kesinlik, bağıl standart sapma olarak hesaplanmış ve sırasıyla % 6.5 ve % 8.4’den daha düşük bulunmuştur. Türkiye’de üretilen toplam 16 bitkisel yağ örneği sentetik antioksidanların varlığını tespit etmek için analiz edilmiş ve örneklerdeki antioksidanların konsantrasyonlarının Türk Gıda Kodeksi Yönetmeliğinde tanımlanan maksimum izin verilen seviyelerin altında kaldığı bulunmuştur.

Anahtar Kelimeler:

Sentetik antioksidanlar, bitkisel yağlar, mikroekstraksiyon, sıvı kromatografisi

LIQUID CHROMATOGRAPHIC DETERMINATION OF SYNTHETIC ANTIOXIDANT CONTENT OF SOME VEGETABLE OILS PRODUCED IN TURKEY

In this work, the synthetic antioxidant contents of some selected vegetable oils produced in Turkey were determined by using liquid chromatography with ultraviolet detector. Butylated hydroxy anisole, butylated hydroxy toluene, and tert-butyl hydroquinone were the synthetic antioxidants selected. The extraction of antioxidants from oil samples was carried out with vortex-assisted liquid-liquid microextraction prior to chromatographic analysis. Some parameters that affect the extraction efficiency, such as the type of extraction solvent, the volume of solvent, and extraction time were optimized. Under the optimized conditions, the limits of detection and limits of quantification for the antioxidants were in the ranges 0.12-0.28 µg/g and 0.40-0.92 µg/g, respectively. The intra-day and inter-days precisions were calculated in terms of relative standard deviation and found to be less than 6.5% and 8.4%, respectively. Totally, 16 vegetable oil samples produced in Turkey were analyzed to detect the presence of the synthetic antioxidants and the concentrations of antioxidants in samples were found far below the maximum permitted levels defined in Turkish Food Codex Regulation.

Keywords:

Synthetic antioxidants, vegetable oils, microextraction, liquid chromatography,

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