AUREOBASIDIUM PULLULANS İLE KSİLANAZ ÜRETİMİNE ETKİ EDEN BİYOPROSES PARAMETRELERİN BELİRLENMESİ

Ksilanazlar, gıda ve yem sektörlerinde çok yaygın olarak kullanılan enzimler arasındadır. Bu çalışmada, farklı Aureobasidium pullulans suşları ksilanaz üretimleri açısından taranmış ve ilgili suşlar arasında en yüksek enzim üretimi A. pullulans Y-2311-1 suşu ile elde edilmiştir. Söz konusu suş ile ksilanaz üretimi üzerine etkisi olan parametreler ve bu parametrelerin optimum değerleri belirlenmiştir. Maksimum enzim üretimi biyoprosesin dördüncü gününde elde edilmiştir. Enzim üretimi için optimum sıcaklık, başlangıç ortam pHsı ve karıştırma hızı sırasıyla 28ºC, pH 3.0 ve 200 rpm olarak belirlenmiştir. Enzim üretimi için en uygun azot kaynağı olarak maya ekstraktı tespit edilmiştir. Optimum koşullarda elde edilen enzim aktivitesi değeri 2527 ± 230 U/ml’dir.

Anahtar Kelimeler:

Ksilanaz, Aureobasidium pullulans, biyoproses

DETERMINATION OF THE BIOPROCESS PARAMETERS EFFECTING XYLANASE PRODUCTION BY AUREOBASIDIUM PULLULANS

Xylanases are one of the leading enzymes within the enzymes used in food and feed industries. In this work, different Aureobasidium pullulans strains have been screened for their ability to produce xylanase and A. pullulans Y-2311-1 was found to be the best strain for xylanase production within the strains tested. Effects of different cultivation parameters on xylanse production by this strain have further been evaluated. Maximum enzyme production was obtained at the fourth day of the bioprocess. The optimum levels of incubation temperature, initial medium pH and agitation speed were found as 28ºC, pH 3.0 and 200 rpm, respectively. Under optimized conditions the enzyme activity reached to 2527 ± 230 U/ml.

Keywords:

Xylanase, Aureobasidium pullulans, bioprocess,

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