Serhat GÜROCAK, Ahmet CUMAOĞLU, İyimser ÜRE, Adnan MENEVŞE, İpek IŞIK GÖNÜL, Aysel ARICIOĞLU, İbrahim BOZKIRLI, Volkan ERGİN

Ureter, the Guardian Angel of Nephron: The Impact of Pelvicalyceal System on Apoptotic Activity in an Experimental Obstructive Uropathy Model

Bu çalışmamızda deneysel obstrüktif üropati modeli uyguladığımız tavşanlarda pelvikalisiyel sistemin renal apoptotik aktivite üzerine etkisini değerlendirmeyi amaçladık. Yöntemler: Yirmi altı adet tavşan obstrüktif üropatinin seviyesine göre 4 gruba ayrıldı. Birinci ve ikinci gruba üreteropelvik (UP) darlık modeli uygulandıktan sonra 1.gruba postoperatif 30.günde bilateral nefrektomi uygulandı. İkinci gruba postoperatif 30.gün piyeloplasti uygulandıktan 30 gün sonra bilateral nefrektomi yapıldı. Üçüncü ve 4. gruba ise üreterovezikal (UV) darlık modeli uygulandıktan sonra 3.gruba postoperatif 30.günde bilateral nefrektomi üreteroneosistostomi uygulandıktan 30 gün sonra bilateral nefrektomi yapıldı. Nefrektomi materyallerindeki apoptotik aktivitenin incelenmesi amacıyla western blot yöntemleriyle apoptotik genlerin protein düzeyinde ifadelenmeleri çalışıldı. Bulgular: UP ve UV obstrüksiyon uygulandığında her iki grupta da apoptotik aktivite yükselmesine rağmen UV obstrüksiyondaki artış UP obstrüksiyona göre daha azdı (p:0.023, p

Nefronun Koruyucu Meleği Üreter: Deneysel Obstrüktif Üropati Modelinde Pelvikalisiyel Sistemin Apoptotik Aktivite Üzerine Etkisi

We aimed to assess the impact of obstructive uropathy and its revision on renal apoptotic and inflammatory activity in an experimental model in rabbits. Methods: Twenty-four rabbits were separated into four different groups. Ureteropelvic junction (UPJ) obstruction model was performed in Group 1 and 2. Ureterovesical junction (UVJ) obstruction model was performed in Group 3 and 4. Thirty days after the operations, bilateral nephrectomy was performed in Group 1 and 3. Thirty days after the initial surgery, the obstruction was revised in Group 2 and 4. Thirty days after the revision surgeries, bilateral nephrectomy was performed in Group 2 and 4. The apoptotic and inflammatory activity was measured at the protein level and nephrectomy specimens were examined histologically. Results: Caspase 3 levels were significantly higher in operated left kidneys than the levels obtained in the right counterparts. The increase in procaspase 9 and caspase 3 activities in the UVJ obstruction group was less than those of the UPJ obstruction group (p

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