Emine YEŞİLYURT, Nur ŞAHİN, Seyyal ROTA, Işıl FİDAN, Zübeyde LALE

Urinary Cytokine Response In BK Virus Infection

İnsan BK virüs (BKV) polyomavirus ailesinin bir üyesidir. Böbrek transplantasyonu yapılmış hastalarda, BKV reaktivasyonu böbrek allograft disfonksiyonuna neden olabilir. Primer BKV infeksiyonu genellikle çocukluk döneminde asemptomatik olarak geçirilir. Primer infeksiyon sonrası, BKV özellikle ürogenital sistemde latent olarak kalır. Hücresel bağışıklık, BKV infeksiyonu patogenezinde önemli rolSakarya Üniversitesi, Eğitim ve Araştırma Hastanesi, Nöroloji Kliniği, Sakarya, Türkiyee sahiptir. Çalışmamızın amacı, BKV infeksiyonlu hastalarda idrar sitokin yanıtını belirlemek ve idrar sitokin düzeyleri ile BK virüri ilişkisini tespit etmektir. Yöntemler: BKV infeksiyonlu 72 hastanın idrar örnekleri çalışma kapsamına alındı. BKV DNA'sı kantitatif Gerçek Zamanlı Polimeraz Zincir Reaksiyonu yöntemi kullanılarak belirlendi. İdrar örneklerinde sitokin düzeyleri ELISA yöntemi ile tespit edildi. Bulgular: BKV DNA'sı pozitif hastalarda idrar proinflamatuvar sitokin düzeyleri, BKV DNA negatif hastalara göre istatistiksel olarak anlamlı düzeylerde daha yüksek olarak tespit edildi. Ayrıca, idrar proinflamatuvar sitokin düzeyleri, yüksek viral yüke sahip BKV DNA'sı pozitif hastalarda düşük viral yüke sahip hastalara göre daha yüksekti. Sonuç: Çalışmamızın sonuçlarına göre, proinflamatuvar sitokinlerin, BKV infeksiyonu patogenezinde önemli rol oynayabileceği düşünülmüştür. BKV infeksiyonunda sitokin yanıtının tam olarak anlaşılması özellikle transplantasyon hastalarında BKV infeksiyonunun tedavisi için yeni yaklaşımların geliştirilmesine yardımcı olabilecektir.

BK Virus İnfeksiyonunda İdrar Sitokin Yanıtı

Human BK virus (BKV) is a member of the polyomavirus family. In renal transplant recipients, reactivation of BKV may cause the renal-allograft dysfunction. Primary BKV infection usually occurs asymptomatically during childhood. After primer infection, BKV persists latently especially in the urogenital system. Cellular immunity plays an important role in the pathogenesis of BKV infection. The aim of this study was to determine the urinary cytokine responses in patients with BKV infection and associated levels of urinary cytokines and BK viruria. Methods: Urine samples of 72 patients with BKV infection were included in this study. BKV DNAs were detected by using quantitative real-time polymerase chain reaction. The levels of cytokines in urine samples were determined by ELISA. Results: The levels of urinary proinflammatory cytokines in BKV DNA positive patients were significantly higher than those of patients who were BKV DNA negative. In addition, urinary proinflammatory cytokines were also higher in BKV DNA positive patients with high viral load than in patients with low viral load. Conclusion: According to our results, it is suggested that proinflammatory cytokines may play an important role in the pathogenesis of BKV infections. A better understanding of cytokine responses in BKV infections may help to provide new therapeutic approaches to the treatment of BKV infection especially in transplantation patients.

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