Ratlarda Metotreksat ile Oluşturulan Karaciğer ve BöbrekHasarı Üzerine Eskuletin’in Etkileri

Bu çalışmada metotreksat (MTX) ile ratlarda oluşturulan karaciğer ve böbrek hasarı üzerineEskuletin’in etkisinin araştırılması amaçlandı. Çalışma Grup 1 (Kontrol grubu), Grup 2 (MTX), Grup3 (Eskuletin) ve Grup 4 (MTX+Eskuletin) olmak üzere toplam 4 gruptan oluştu. Grup 3 ve 4’dekiratlara 14 gün süre ile 100 mg/kg dozda eskuletin uygulandı. Ayrıca Grup 2 ve 4’deki ratlaradenemenin 9. gününde 20 mg/kg dozda intraperitonal olarak MTX enjekte edildi. MTX uygulamasından 5 gün sonra çalışma sonlandırıldı. Çalışma sonunda, MTX grubunda karaciğer veböbrek dokusu malondialdehit (MDA) düzeylerinin arttığı, karaciğerde redukte glutatyon (GSH)düzeyinin azaldığı, her iki dokuda glutatyon peroksidaz (GSH-Px) ve katalaz (CAT) enzimaktivitelerinin ise önemli düzeyde azaldığı ve MTX uygulamasının bu dokularda oksidatif streseneden olduğu tespit edildi. Eskuletin ile tedavi edilen gruplarda karaciğer ve böbrek dokusu MDAseviyelerinin azaldığı, GSH-Px ve CAT aktivitelerinin arttığı belirlendi. Ayrıca eskuletinuygulamasının artan serum alanin transaminaz (ALT) ve aspartat transaminaz (AST) seviyelerinide azalttığı görüldü. Histopatolojik analizlerde ise MTX’in hem karaciğer hem böbrekte hidropikdejenerasyon, yangısal hücre infiltrasyonu gibi dejeneratif ve yangısal değişikliklere sebep olduğu;eskuletin uygulamasının bu lezyonları önemli miktarda azalttığı tespit edildi. Sonuç olarakeskuletinin, antioksidan ve antiinflamatuvar etkileri ile MTX’in sebep olduğu karaciğer ve böbrekhasarını önlediği veya minimize ettiği belirlendi.

Effects of Esculetin on the Methotrexate Induced Liver and Kidney Damage in Rats

In this study, it was aimed to investigate the effect of Esculetin on liver and kidney damage inducedby methotrexate (MTX) in rats. Our study consisted of 4 groups: Group 1 (Control group), Group 2(MTX), Group 3 (Esculetin) and Group 4 (MTX+Esculetin). Groups 3 and 4 received a dose of 100mg / kg esculetin by orally during 14 days. In addition, groups 2 and 4 received MTX at a dose of20 mg / kg intraperitoneally on the 9th day of the experiment. The study was terminated 5 daysafter the MTX application. Liver and kidney tissue MDA levels increased, GSH level and CAT andGSH-Px enzyme activities significantly decreased in MTX group and MTX administration causedoxidative stress in these tissues. Esculetin administration reduced the MDA level in liver and kidneytissues and increased the CAT and GSH-Px enzyme activities. In addition, esculetin administrationdecreased serum AST, ALT. At the histopathological examination, MTX caused degenerative andinflammatory changes such as hydropic degeneration and inflammatory cell infiltration in both liverand kidney. It was determined that esculetin significantly reduced these lesions. In conclusion,esculetin can prevent or minimize MTX induced liver and kidney damage due to its antioxidant andanti-inflammatory activities.

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