Özgül GÜLAYDIN, Kemal GÜRTÜRK, İsmail Hakkı EKİN, Berivan KAPLAN

Van ve Yöresinde Sığır Sütlerinden İzole Edilen Koagulaz Negatif Stafilokokların Bazı Antimikrobiyal Maddelere KarşıDuyarlılığının Belirlenmesi

Bu çalışmada, Van ve yöresinde süt sığırı işletmelerindeki mastitis şüpheli vakalardan alınan sütörneklerinden izole edilen koagulaz negatif Staphylococcus spp. suşlarının bazı antimikrobiyalmaddelere karşı duyarlılıkları araştırıldı. İzolatların tür düzeyinde identifikasyonları konvansiyonelbakteriyolojik yöntemlerle, antimikrobiyal duyarlılıkları ise disk difüzyon yöntemiyle belirlendi.Çalışmada en yüksek oranda izole edilen koagulaz negatif stafilokok (KNS) türününStaphylococcus vitulinus olduğu ve bunu sırasıyla S. epidermidis, S. xylosus ve S. warneri izolatlarının izlediği belirlendi. İzolatlarda en yüksek oranda penisilin (%34.61) ve sefoksitin(%20.19) direnci tespit edilmekle birlikte, izolatların tamamı trimetoprim+ sulfametoksazole duyarlıbulundu. Araştırmada incelenen izolatlarda çoklu antibiyotik direncinin de sınırlı sayıda (%4.81)olduğu görüldü. Sonuç olarak, doğal florada bulunabildiği gibi mastitis vakalarından da sıklıkla izoleedilen koagulaz negatif Staphylococcus spp. izolatlarında beta-laktam grubu antibiyotiklere karşıdirencin, diğer antibiyotik gruplarına göre daha yüksek olduğu belirlendi. Söz konusu etkenlerinneden olduğu enfeksiyonların tedavisinde beta-laktam grubu antibiyotiklerin kullanımına dikkatedilmesi gerektiği kanaatine varıldı.

Determination of Antimicrobial Susceptibility of Coagulase Negative Staphylococci Strains Isolated from Bovine Milk in Van Province

In this study, the antimicrobial susceptibility of coagulase negative Staphylococcus spp. strainsisolated from milk samples collected from cows with mastitis in dairy cattle farms in Van provincewere investigated. Identification of the isolates at the species level was determined by conventionalbacteriological methods, and antimicrobial susceptibility was determined by disc diffusion method.In the study, it was determined that the most isolated coagulase-negative staphylococcus (CNS)strain was Staphylococcus vitulinus, followed by S. epidermidis, S. xylosus and S. warneri isolates,respectively. Although the highest rate of penicillin (34.61%) and cefoxitin (20.19%) resistance wasfound in isolates, all isolates were found to be susceptible to trimethoprim+sulfamethoxazole.Multidrug resistance was found in a limited number (4.81%) in the isolates examined in the study.As a result, it was determined that resistance to beta-lactam group antibiotics was higher incoagulase negative Staphylococcus spp. isolates, which can be found in the natural flora as well asfrequently isolated from mastitis cases, compared to other antibiotic groups. It was concluded thatthe use of beta-lactam group antibiotics should be re-considered in the treatment of infectionscaused by these bacterial agents.

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1. Sasshofer A, Loibl A, Kessler O. Erkankungen be i Schaf und Ziege. 7. Euterentzündungen. Wien Tierarztl Mschr 1987; 4: 125-131.
2. Tenhagen BA, Köster G, Wallmann J, Heuwieser W. Prevalence of mastitis pathogens and their resistance against antimicrobial agents in dairy cows in Brandenburg, Germany. J Dairy Sci 2006; 89: 2542-2551.
3. Lundberg A, Nyman AK, Aspan A, et al. Udder infections with Staphylococcus aureus, Streptococcus dysgalactiae, and Streptococcus uberis at calving in dairy herds with suboptimal udder health. J Dairy Sci 2016; 99: 2102- 2117.
4. Wang Y, Wu CM, Lu LM, et al. Macrolide–lincosamideresistant phenotypes and genotypes of Staphylococcus aureus isolated from bovine clinical mastitis. Vet Mic 2008; 130: 118-125.
5. Quinn PJ, Markey BK, Leonard FC, et al. Veterinary Microbiology and Microbial Disease. 2nd Edition, UK: John Wiley & Sons Ltd, 2011.
6. Samanta I, Bandyopadhyay S. Antimicrobial Resistance in Agriculture. Netherlands: Academic Press, 2020.
7. Ünal N. Mastitisli hayvanlardan izole edilen stafilokokların antibiyotik direnci ve hayvanlarda metisiline dirençli Staphylococcus aureus. Erciyes Üniv Vet Fak Derg 2012; 9: 221-231.
8. Adwan G, Abusafieh D, Aref R, Omar JA. Prevalence of microorganisms associated with intramammary infectıon in cows and small ruminants in the north of Palestine. IUGNS 2005; 13: 165-173.
9. Kırkan Ş, Göksoy EÖ, Kaya O. Identification and antimicrobial susceptibility of Staphylococcus aureus and coagulase negative staphylococci from bovine mastitis in the Aydın region of Turkey. Turk J Vet Anim Sci 2005; 29: 791-796.
10. Lüthje P, Schwarz S. Antimicrobial resistance of coagulase-negative staphylococci from bovine subclinical mastitis with particular reference to macrolidelincosamide resistance phenotypes and genotypes. J Antimicrob Chemother 2006; 57: 966-969.
11. Taponen S, Pyörala S. Coagulase-negative staphylococci as cause of bovine mastitis-Not so different from Staphyloccoccus aureus? Vet Mic 2009; 134: 29-36.
12. Mariela ES, Yannick DNT, Josee L. Biofilm formation and antimicrobial resistance genes of coagulase-negative staphylococci isolated from cows with mastitis in Argentina. FEMS Microbiol Lett 2017; 364: 1-8.
13. Archer GL, Climo MW. Antimicrobial susceptibility of coagulase-negative staphylococci. Antimicrob Agents Chemother 1994; 38: 2231-2237.
14. Sawant AA, Sordillo LM, Jayarao BM. A survey on antibiotic usage in dairy herds in Pennsylvania. J Dairy Sci 2005; 88: 2991-2999.
15. Jensen SO, Lyon BR. Genetics of antimicrobial resistance in Staphylococcus aureus. Future Microbiol 2009; 4: 565- 582.
16. Türütoğlu H, Erçelik S, Öztürk D. Antibiotic resistance of Staphylococcus aureus and coagulase-negative staphylococci isolated from bovine mastitis. Bull Vet Inst Pulawy 2006; 50: 41-45.
17. Ünal N, İstanbulluoğlu E. İnsan ve sığır kökenli Staphylococcus aureus izolatlarının fenotipik ve genotipik özelliklerinin araştırılması. Ankara Univ Vet Fak Derg 2009; 56: 119-126.
18. Macun HC, Pir Yağcı İ, Ünal N, ve ark. Kırıkkale’de belirlenen subklinik mastitisli ineklerde etken izolasyonu ve antibiyotik direnç durumu. Erciyes Üniv Vet Fak Derg 2011; 8: 83-89.
19. Tel OY, Keskin O. Subklinik mastitisli ineklerden izole edilen stafilokok suşlarının bazı virulens faktörleri ve antibiyotik direnci. YYU Vet Fak Derg 2011; 22: 17-21.
20. Carter GR. Pasteurella, Yersinia and Francisella. In: Diagnostic Procedures in Veterinary Bacteriology and Mycology. 4th Edition, USA: Charles C Thomas 1984: 111-121.
21. Koneman EW, Allen SD, Dowel VR, et al. Color Atlas and Textbook of Diagnostic Microbiology. 3rd Edition, Philadelphia: Lippincott Comp, 1988.
22. Bauer AW, Kirby WMM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Pathol 1966; 45: 493-494.
23. CLSI. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals. 4th Edition, Pennsylvania: CLSI 2018.
24. CLSI. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals approved standard. 2nd Edition, Pennsylvania: CLSI 2002.
25. EUCAST. Breakpoint tables for interpretation of MICs and zone diameters. 9th Edition, Switzerland: EUCAST 2019.
26. Magiorakos AP, Srinivasan A, Carey RB, et al. Multidrug resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect 2012; 18: 268-281.
27. Ünal NR, Besler T. Beslenmede Sütün Önemi. 1. Baskı, Ankara: Klasmat Matbaacılık, 2012.
28. Sevinti DA, Şahin M. Sığır mastitislerinden izole edilen stafilokok suşlarının beta-laktamaz aktivitesi ve bazı antibiyotiklere karşı duyarlılıklarının saptanması. Vet Bil Derg 2009; 25: 23-28.
29. Çelik A, Solmaz H. Investigation of antibiotic susceptibility and presence of plasmids in staphylococci isolated from cow milk with subclinical mastitis. YYU Veteriner Fakültesi Dergisi 2010; 21: 141-145.
30. Kaynarca S, Türkyılmaz S. Sığır mastitislerinden izole edilen stafilokoklarda metisilin direnci ve slaym pozitifliği. Kafkas Univ Vet Fak Derg 2010; 16: 567-572.
31. Kaliwal BB, Sadashiv SO, Kurjogi MM, Sanakal RD. Prevalence and antimicrobial susceptibility of coagulasenegative staphylococci isolated from bovine mastitis. Vet World 2011; 4: 158-161.
32. Klibi A, Maaroufia A, Torres C, Jouini A. Detection and characterization of methicillin-resistant and susceptible coagulase-negative staphylococci in milk from cows with clinical mastitis in Tunisia. Int J Antimicrob Ag 2018; 52: 930-935.
33. Kenar B, Kuyucuoğlu Y, Şeker E. Antibiotic susceptibility of coagulase-negative staphylococci isolated from bovine subclinical mastitis in Turkey. Pak Vet J 2012; 32: 390- 393.
34. Nam HM, Lim SK, Kim JM. Antimicrobial susceptibility of coagulase-negative staphylococci isolated from bovine mastitis between 2003 and 2008 in Korea. J Microbiol Biotechnol 2010; 20: 1446-1449.
35. Frey Y, Rodriguez JP, Thomann A, Schwendener S, Perreten V. Genetic characterization of antimicrobial resistance in coagulase-negative staphylococci from bovine mastitis milk. J Dairy Sci 2013; 96: 2247-2257.
36. Jiménez Velásquez SC, Higuera LDT, Arangob JLP. Profile of antimicrobial resistance in isolates of Staphylococcus spp. obtained from bovine milk in Colombia. Rev Argent Microbiol 2020; 52: 121-130.
37. Kirby WM. Extraction of a highly potent penicillin inactivator from penicillin resistant staphylococci. Science 1944; 99: 452-453.
38. Blair JE, Carr M. Distribution of phage groups of Staphylococcus aureus in the years 1927 through 1947. Science 1960; 132: 1247-1248.
39. Ünal N, Yıldırım M. İneklerin süt, meme başı derisi ve burun mukozalarından izole edilen stafilokok türlerinin antibiyotik direnç profilleri. Kafkas Univ Vet Fak Derg 2010; 16: 389-396.
40. Şeker E, Özenç E. Mastitisli inek sütlerinden izole edilen koagulaz negatif stafilokokların antibiyotik dirençlilikleri. YYU Veteriner Fakultesi Dergisi 2010; 21: 107-111.
41. Soares LC, Pereira IA, Pribul BR. Antimicrobial resistance and detection of mecA and blaZ genes in coagulasenegative Staphylococcus isolated from bovine mastitis. Pesq Vet Bras 2012; 32: 692-696.
42. Singh B, Kumar R. Antimicrobial susceptibility of coagulase negative Staphylococci isolates from suspected cases of bovine subclinical mastitis in parts of Bundelkhand region. Asian Pac J Health Sci 2018; 5: 100- 107.
43. Gizaw F, Kekeba T, Teshome F. Distribution and antimicrobial resistance profile of coagulase-negative staphylococci from cattle, equipment, and personnel on dairy farm and abattoir settings. Heliyon 2020; 6: e03606.