Koç Spermasına Katılan Bazı Antioksidanların Dondurma ve Çözdürme Sonrası Spermatolojik Parametreler, Oksidatif Stres ve DNA Hasarı Üzerine Etkileri

Bu çalışmada, spermaya katılan antioksidanların dondurma çözdürme sonrası spermatolojik, bigisayar destekli sperma analiz cihazı (CASA), oksidatif stres parametreleri ve DNA hasarları üzerine etkilerinin belirlenmesi amaçlandı. Afyonkarahisar koşullarında yetiştirilen 2-3 yaşlı Pırlak ırkına ait toplam 10 koçtan alınan spermalar birleştirilip 5 eşit hacme bölünerek kontrol başta olmak üzere taurin, trehaloz, trolox ve lipoik asit içeren Tris bazlı sulandırıcılar ile sulandırıldı. Sulandırılan örnekler 0.25 mL’lik payetlerde 5 0C’de 3 saat ekilibrasyona tabi tutulduktan sonra sıvı azot buharında dondurularak sıvı azot içerisinde (-196 ⁰ C) saklandı. Çözdürülen spermalarda subjektif motilite, CASA motilitesi, ortalama rota hızı μm/s (VAP) ve düz çizgi hızı μm/s (VSL) açısından taurin grubunda en yüksek değerler tespit edildi. Anormal spermatozoon baş, orta kısım ve akrozom oranı açısından kontrol grubuna göre tüm gruplarda en düşük değerler elde edildi. Spermatozoon hipo-ozmotik eosin boyama testi (HE-test) parametrelerinden H+/E- tip spermatozoonlarda kontrol grubuna göre taurin, trehaloz ve lipoik asit gruplarındaki artışlar önemli (P

Effects of Some Antioxidant Additives on Spermatological Parameters, Oxidative Stress and DNA Damage After Freezing-Thawing Process in Ram Semen

In this study, determing the effects of antioxidants on the computer assisted sperm analysis(CASA), oxidative stress parameters and the DNA damage of the thawed sperm was aimed.Semen collected from 10 Pirlak rams was divided into five equal aliquots and diluted with the trisbase extender containing taurine, trehalose, trolox, lipoic acid and no antioxidant (control), cooledto 5 ⁰ C and frozen in 0,25 mL French straws in liquid nitrogen vapour and stored in liquid nitrogen.In the thawed semen, the highest values were detected in taurine group with regard to subjectivemotility and CASA motility, average path velocity μm/s (VAP) and straight linear velocity μm/s(VSL). The lowest values in spermatozoa with abnormal head, middle piece and acrosome werefound in all antioxidant groups when compared with the control group. The increases inhypoosmotic eosin staining test (H+/E- type) spermatozoa were significant (P

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