Mustafa Reha COŞKUN, Elif ÇELİK, Özgür ÇELEBİ, Aliye GÜLMEZ SAĞLAM, Salih OTLU, Mitat ŞAHİN, Fatih BÜYÜK

Yaban ördeklerinden (Anas platyrhynchos) termofilik Campylobacter türlerinin izolasyonu ve moleküler tanımlanmasının değerlendirilmesi

Amaç: Bu çalışmada, yaban ördeklerinde insan ve hayvan sağlığını olumsuz yönde etkileyen Campylobacter türlerinin araştırılması amaçlandı. Gereç ve Yöntem: Yaban ördeklerinden toplanan 110 dışkı örneği kültürel ve moleküler yöntemlerle incelendi. Ön zenginleştirme amacıyla örnekler Preston Campylobacter Enrichment Broth’a ekildi ve inkübe edildi. İnkübasyondan sonra ön zenginleştirme kültürden Preston Campylobacter Selective Agar’a ekim yapıldı ve 42°C 24-48 saat inkübasyona bırakıldı. Üremenin olduğu kültürler önce koloni morfolojisi ve sonra mikroskobik görünüm açısından değerlendirildi. Şüpheli koloniler değerlendirildikten sonra multiplex PCR ile incelendi. Bulgular: Toplam 110 dışkı örneğinin 10'unda (%9,1) Campylobacter spp. bulundu. Yapılan Multiplex PCR ile 10 izolatın Campylobacter jejuni olduğu teyit edilmiştir. Öneri: Bu çalışma ile, yaban ördeklerinden Campylobacter spp. varlığı belirlendi ve yaban ördeklerinin Campylobacter türleri için önemli bir rezervuar olabileceği kanaatine varıldı. Ayrıca, Kars bölgesi kuşların göç güzergahında olduğu için göçmen kuşlarının potansiyel taşıyıcı olabilecekleri göz önünde tutulmalıdır.

Isolation and molecular identification of thermophilic Campylobacter species from mallard (Anas platyrhynchos)

Aim: The aim of this study was to investigate the Camplyobacterspecies which adversely affect human and animal health in mallard.Materials and Methods: 110 stool samples were collected fromthe mallard and examined by the cultural and molecular method.For pre-enrichment step, samples were inoculated with PrestonCampylobacter Enrichment Broth. At the end of the incubation, preenrichedculture was inoculated on Preston Campylobacter SelectiveAgar and the plates were incubated for 48-72 hours at 42°C. The culturesin which the growth was observed were first evaluated for thecolony morphology and then for microscopic appearance. Suspectedcolonies were examined with multiplex PCR.Results: In this study, Campylobacter spp. was found in 10 (9.1%) ofthe 110 stool specimens. All 10 isolates were typed as Campylobacterjejuni by multiplex PCR.Conclusion: In this study, Campylobacter was detected in mallardsand it was concluded that mallard may be an important reservoir forCampylobacter species. Besides, since Kars area is on the migrationroute of birds, it is important to examine the migratory birds and todetermine the infections which birds have the potential.

PDF

___

Aarestrup FM, Engberg J, 2001. Antimicrobial resistance of thermophilic Campylobacter. Vet Res, 32, 311-321.
Arikoğlu C, Aydin F, 2000. The prevalence of Campylobacter jejuni in domestic and wild animals in Kars district. Kafkas Univ Vet Fak Derg, 3, 173-180.
Colles FM, Dingle KE, Cody AJ, Maiden MCJ, 2008. Comparison of Campylobacter populations in wild geese with those in starlings and free-range poultry on the same farm. Appl Environ Microbiol, 74, 3583-3590.
Corry JEL, Atabay HI, 2001. Poultry as a source of Campylobacter and related organisms. J Appl Microbiol, 90, 96-114,
Eurosurveillance Editorial Team, 2012. The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2010. Euro Surveill. 17:20113. http://www.eurosurveillance .org/ViewArticle. aspx?ArticleId20113.
Colles FM, Jan SA, Sheppard SK, Mccarthy ND, et al., 2011. Campylobacter populations in wild and domesticated Mallard ducks (Anas platyrhynchos). Environ Microbiol, 3, 574-580,
Keller JI, Shriver WG, 2014. Prevalence of three Campylobacter species, C. jejuni, C. coli, and C. lari, using multilocus sequence typing in wild birds of the mid-atlantic region, USA. J Wildl Dis, 50, 31-41,
Keller JI, Shriver WG, Waldenström J, Griekspoor P, et al., 2011. Prevalence of Campylobacter in wild birds of the Mid-Atlantic region, USA. J Wildl Dis, 47, 750-754.
Kwon YK, Oh JY, Jeong OM, Moon OK, et al., 2017. Prevalence of Campylobacter species in wild birds of South Korea. Avian Pathol, 46, 474-480.
Mohan V, 2015. Faeco-prevalence of Campylobacter jejuni in urban wild birds and pets in New Zealand. BMC Res. Notes, 8, 1.
Pacha R, Clark GW, Williams EA, Carter AM, 1998. Migratory birds of central Washington as reservoirs of Campylobacter jejuni. Can J Microbiol, 34, 80-82.
Padungton P, Kaneene JB, 2003. Campylobacter spp. in human, chickens, pigs and their antimicrobial resistance. J Vet Med Sci, 65, 161-170.
Sambrook J, Russell D, 2001. Molecular cloning: A laboratory manual. Third ed., Cold Spring Harbor Laboratory Press, America. Skirrow MB, Benjamin J, 1980. '1001' Campylobacters: Cultural characteristics of intestinal Campylobacters from man and animals. J Hyg (Lond), 427-442.
Şeker E, Çelik A, Yardımcı H, 2007. Isolation of thermophilic Campylobacter spp. from feces of cage birds in the city of Ankara. Vet J Ankara Univ, 54, 43-46.
Vandamme P, Goossens H, 1992. Taxonomy of Campylobacter, Arcobacter and Helicobacter. A review. Zentralblatt für Bakteriologie, 276, 447-472.
Wang G, Clark CG, Taylor TM. Pucknell C, et al., 2002. Colony multiplex PCR assay for identification and differentiation of Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, and C. fetus subsp. fetus. J Clin Microbiol, 40, 4744-4747.