Feyzullah BEYAZ, Mehmet ÖZBEK, Hidayet TUTUN, Levent ERGÜN, Emel ERGÜN

Prenatal gelişim boyunca koyun ileumunda kannabinoid reseptör 2’nin ekspresyon profili

Amaç: Bu çalışmada prenatal gelişim boyunca koyun ileumundaki kannabinoid reseptör 2 (CB2)’nin ekspresyonunu araştırmayı amaçladık. Gereç ve Yöntem: Çalışmamızda fötal yaşı 63 ile 147 gün arasında değişen 20 adet koyun fötüsünün ileum örnekleri kullanıldı. CB2 ekspresyonunu göstermek için Streptavidin-Biotin peroksidaz immunohistokimya yöntemini kullandık. Bulgular: Bağırsak epitelinin çoğunluğu CB2 için zayıf bir pozitif reaksiyon gösterdi. İlginç bir şekilde, hem villus hem de kriptlerin bazı epitel hücrelerinde yoğun intrasitoplazmik boyanma gözlemledik. Ayrıca, submukozal ve miyenterik pleksustaki ganglion hücrelerinde CB2 immun reaktivitesi gözlendi. Üstelik, lamina ve tunica muskularisteki düz kas hücrelerinde de pozitif boyanma tespit ettik. Prenatal dönemin sonuna doğru, histolojik olarak olgun Peyer plaklarına rastladık. Follikül ve dom bölgesini oluşturan immune sistem hücrelerinin çoğunda CB2 boyanması tespit edildi. Ayrıca, follikülle ilişkili epitelde CB2 ile pozitif boyandı. Öneri: Bu çalışma fötal koyun ileumda CB2 ekspresyonunu tanımlayan ilk çalışmadır. Fizyolojik koşullar altında, CB2 ekspresyonu fetal koyun ileumdaki epitel hücrelerinde, düz kaslarda, enterik nöronlarda ve Peyer plaklarında belirlendi. CB2 kuvvetli boyanan epitel hücrelerinin morfolojisi ve önceki araştırmacıların bulguları göz önüne alındığında, CB2 prenatal dönemde enteroendokrin hücreler için biyolojik bir belirteç olabilir.

Expression profile of cannabinoid receptor 2 in ovine ileum during prenatal development

Aim: Here, we aimed to explore cannabinoid receptor 2 (CB2) expression in ovine ileum during prenatal development using immunohistochemistry.Materials and Methods: We used 20 ileal samples from fetal sheep whose gestational ages range from 63 to 147 days. To visualize CB2 expression in ileum, we applied labeled Streptavidin Biotin method to paraffin sections.Results: The majority of the intestinal epithelium showed a weak positive reaction for CB2. Interestingly, we observed intense intracytoplasmic staining in some epithelial cells of both intestinal villi and crypts. Moreover, CB2 immunoreaction was observed in ganglion cells in submucosal and myenteric plexus. We also determined positive staining in smooth muscle cells of lamina and tunica muscularis. Towards the end of the prenatal period, we found histologically mature Peyer’s patches. CB2 staining was detected in the majority ofimmune cells forming the follicle and dome region. Follicle associated epithelium also showed CB2 immunoreaction.Conclusion: The present study is the first to describe CB2 expression in fetal sheep ileum. CB2 expression was determined in epithelial cells, smooth muscles, enteric neurons and Peyer’s patches in ileum fetal sheep under physiological conditions. Considering the morphology of epithelial cells with intense CB2 staining and previous findings, CB2 may be a possible marker for enteroendocrine cells during the prenatal period.

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