Manda STAT5A geninin 6-9 eksonları arasındaki polimorfizmlerin belirlenmesi

Amaç: Bu çalışmanın amacı, Anadolu mandalarında, süt verimini ve süt bileşimini etkileyen STAT5A geninin 6. - 9. ekzonları arasındaki polimorfizmleri belirlemektir. Gereç ve Yöntem: Araştırmanın materyalini, Afyonkarahisar bölgesinde yetişen 96 adet Anadolu mandasından alınan kan örnekleri oluşturmuştur. Bulgular: Araştırmada, STAT5A geninin sekizinci ekzonunda g+12221 C>T polimorfizmi tespit edilmiştir. Öneri: Sinyal transdüseri ve transkripsiyon aktivatörü 5A (STAT5A) geni, süt verimini ve bileşimini etkilediği için, süt hayvanı yetiştiriciliğinde oldukça önemlidir, ayrıca hücre içinde de birçok hayati görevi bulunmaktadır. Bu çalışmada bulunan 8. ekzondaki polimorfizm protein sentezinde ki amino asit diziliminde bir değişikliğe neden olmadığından sessiz bir mutasyon olarak nitelendirilmiştir. Islah çalışmalarının daha verimli yapılabilmesi için, memeli canlılarda süt verimi ve süt kompozisyonu üzerine etkili olan STAT5A geniyle ilgili çalışmaların çoğaltılması önem arz etmektedir.

The determination of polymorphisms between 6-9 exons of the water buffalo STAT5A gene

Aim: The aim of this study was to determine the polymorphisms between 6th-9th exons of STAT5A gene which affects milk yield and milkcomposition in Anatolian buffaloes.Materials and Methods: The material of the study was composed of the blood samples taken from 96 Anatolian buffaloes grown in Afyonkarahisarregion.Results: According to the results of the research, g+12221 C> T polymorphism was detected in the 8th exon of STAT5A gene.Conclusion: The signal transducer and transcription activator 5A gene (signal transducers and activators of transcription 5A, STAT5A) is important in dairy animal breeding because it has many vital tasks within the cell as well in addition to affecting milk yield and composition. In this study, the polymorphism in the 8th exon was described as a silent mutation since protein synthesis did not cause a change in amino acid sequence. In order to make the breeding studies more efficient, it is important to increase the studies on STAT5A gene, which affects milk yield and milk composition in mammals.

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Belkhir K, Borsa P, Chikhi L, Goudet J, et al., 1996. Genetix 4.00 windows software for population genetics. Laboratoire genome, populations, intereactions, University of Montpellier, France.
Bromberg JF, Wrzeszczynska MH, Devgan G, Zhao Y, et al., 1999. STAT3 as an oncogene. Cell, 98, 295-303.
Brym P, Kawinski S, Ruse A, 2004. New SSCP polymorphism within bovine STAT5A gene and its associations with milk performance traits in black-and-white and Jersey cattle. J. Appl. Genet., 45 (4), 445 – 452.
Chaix A, Lopez S, Voisset E, Gros L, et al., 2011. Mechanisms of STAT protein activation by oncogenic KIT mutants in neoplastic mast cells. The J.Biol.Chem., 286 (8), 5956-5966.
Dario C, Selvaggi M, 2011. Study on the STAT5A/AvaI polymorphism in Jersey cows and association with milk production traits. Mol Biol Rep, 38, 5387 – 5392.
Doğan AL, Güç D, 2004. Sinyal iletimi mekanizmaları ve kanser. Hacettepe Tıp Dergisi, 35, 34 – 42.
Flisikowski K, Zwierzchowski L, 2003, Polymerase chain reaction- heteroduplex (PCR-HD) polymorphism within the bovine STAT5A gene. J. Appl. Genet., 44 (2), 185 – 189.
Flisikowski K, Strzalkowska N, Sloniewski K, Krzyzewki J, et al., 2004. Association of a sequence nucleotide polymorphism in exon 16 of the STAT5A gene with milk production traits in Polish black and white (Polish Friesian) cows. Anim. Sci. Pap. Rep., 22 (4), 515 – 522.
Frasor J, Gibori G, 2003. Prolactin regulation of estrogen receptor expression. Trends Endocrin. Met., 14 (3), 118 – 123.
Goldammer T, Meyer L, Seyfert HM, Brunner RM, et al., 1997. STAT5A encoding gene maps to chromosome 19 in cattle and goat and to chromosome 11 in sheep. Mamm. Genome, 705 – 706.
Hall TA, 1999. BioEdit: A user-friendly biological sequen- ce alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids. Symp. Ser., 41, 95 – 98.
He X, Chu MX, Qiao L, He JN, et al., 2012. Polymorphisms of STAT5A gene and their association with milk production traits in holstein cows. Mol Biol Rep, 39, 2901 – 2907.
Hennighausen L, Robinson GW, 2008. Interpretation of cytokine signaling through the transcription factors STAT5A and STAT5B. Genes Dev., 22, 711 – 721.
Ihle JN, 1996. STATs: Signal transducers and activators of transcription. Cell, 84, 331 – 334.
Ihle JN, 2001. The STAT family in cytokine signaling. Current Opinion in Cell Biology, 13, 211–217.
Kale DS, Yadav BR, 2012. PCR-SSCP within STAT5A gene and its association with milk yield in Murrah buffalo. Indian Vet. J., 89 (11), 16 – 20.
Kalendar R, Lee D, Schulman AH, 2009. FastPCR software for pcr primer and probe design and repeat search. Genes, Genomes and Genomics, 3 (1), 1 – 14.
Khatib H, Maltecca C, Monson RL, Schutzkus V, et al., 2009. Monoallelic maternal expression of STAT5A affects embryonic survival in cattle. BMC Genetics, 10, 13.
Kisseleva T, Bhattacharya S, Braunstein J, Schindler CW, 2002. Signaling through the Jak/STAT pathway, recent advances and future challenges. Gene, 285, 1 – 24.
Küçükkebabçı M, Aslan S, 2002. Evcil dişi mandaların üreme özellikleri, Lalahan Hay. Arast. Enst. Derg., 42 (2), 55 – 63.
Li S, Huang S, Qiao S, Jiang J, et al., 2018. Cloning and functional characterization of STAT5a and STAT5b genes in buffalo mammary epithelial cells. Anim Biotechnol., https://doi. org/10.1080/10495398.2018.1538014
Liu XF, Li M, Li QZ, Lu LM, et al., 2012. STAT5A increases lactation of dairy cow mammary gland epithelial cells cultured in vitro. In Vitro Cell. Dev. Biol. Animal, 48, 554 – 561.
Mccracken JY, Molenaar AJ, Snell RJ, Davey HW, et al., 1997. A polymorphic TG repeat present within the bovine STAT5A gene. Anim. Genet., 28, 453 – 461.
Paramitarasi K, Sumantri A, Jakaria C, 2015. The genetic variability of prolactin and signal transducers and activators of transcription 5A (STAT5A) genes in Bali cattle, Media Peternakan, 38 (1), 1 – 11.
Raven LA, Cocks BG, Goddard ME, Pryce JE, et al., 2014. Genetic variants in mammary development, prolactin signalling and involution pathways explain considerable variation in bovine milk production and milk composition. Genet. Sel. Evol., 46, 1 – 29.
Selvaggi M, Dario C, Normanno G, Celano GV, et al., 2009. Genetic polymorphism of STAT5A protein: relationships with production traits and milk composition in Italian Brown Cattle. J. Dairy Res., 76, 1 – 5.
Şahin A, Ulutaş Z, Yıldırım A, 2013. Türkiye ve Dünya’da manda yetiştiriciliği. Gaziosmanpaşa Journal of Scientific Research, 8, 65 – 70.
Şekerden Ö, Erdem H, Kankurdan B, Özlü B, 1999. Anadolu Mandalarında süt kompozisyonunu etkileyen faktörler ve süt kompozisyonunun laktasyon dönemlerine göre degişimi. Turk J Vet Anim Sci, 23, 505-509.
Yu H, Jove R, 2004. The STATs of cancer – new molecular targets come of age. Nat. Rev. Cancer, 4 (2), 97 – 105.