Zelal KARAKOÇ, Muzaffer Aydın KETANİ, Uğur TOPALOĞLU

Kıl keçisi abomasumunda ghrelin, obestatin ve leptin hormonlarının immunohistokimyasal dağılımları

Amaç: Ghrelin, obestatin ve leptin hormonları iştah düzenleyici hormonolarak adlandırılırlar. Ghrelinin yemeyi başlattığı, obestatininiştahı baskıladığı ve leptinin tokluk hissi verdiği bildirilmiştir. Yapılançalışmalarda insan ve diğer memeli türlerinde ghrelinin, obestatininve leptinin midenin tüm bölümlerinden değişik yoğunluklardaeksprese olduğu bildirilmiştir. Sunulan çalışma kıl keçisi abomazumundaghrelin, obestatin ve leptin hormonlarının ekspresyonununbelirlenmesi amacıyla yapılmıştır.Gereç ve Yöntem: Çalışmada 6-8 aylık oğlaklara ait abomazum dokularıtemin edilip, abomazumun kardiya, fundus ve pilorus bölgelerindendoku parçaları alındı. Rutin histolojik takipten sonra alınankesitlere ghrelin, obestatin ve leptin immunohistokimya boyamalarıuygulandı.Bulgular: Yapılan histolojik değerlendirmede abomazumun her üçbölgesinde ghrelin, obestatin ve leptin için değişik yoğunlukta pozitifreaksiyon veren hücrelere rastlandı. Kardiya bölgesinde immunoreaktiviteninabomazum boyunca dağınık bir yerleşim gösterdiğive fundus bölgesinde immunoreaktivitenin özellikle pariyetal hücrelerdeolduğu belirlendi. Ortalama H skoruna göre ghrelinin kardiyabölgesinde, obestatinin pilorus bölgesinde ve leptinin fundus bölgesindekihücrelerde yoğun olarak eksprese olduğu belirlendi.Öneri: İştah düzenleyici hormonlar olan ghrelin, obastatin ve leptininkıl keçilerinin abomazumunda kardiya, fundus ve pilorus bölgelerindende değişik yoğunluklarda eksprese olması bu hormonlarınyem alımı üzerindeki fizyolojik etkileri göz önüne alındığında kıl keçilerindede benzer fonksiyonları yerine getirdiğini düşündürmüştür.Ancak ghrelin, obestatin ve leptin hormonlarının hayvan beslenmesindekullanımı ile ilgili daha fazla araştırmaya ihtiyaç vardır.

Immunohistochemical distribution of ghrelin, obestatin and leptin hormones in the abomasum of hair goat

Aim: Ghrelin, obestatin and leptin hormones are called appetite regulating hormones. It reported that ghrelin initiates feeding, obestatin suppresses appetite and leptin gives a feeling of fullness. In human and other mammalian species, studies have reported that ghrelin, obestatin and leptin are expressed at different concentrations from all parts of the stomach. The present study was conducted to determine the expression of ghrelin, obestatin and leptin hormones in hair goat abomasum. Materials and Methods: The abomasum tissues for the study were obtained from 6-8-month-old kids and were collected from cardia, fundus and pylorus regions of the animals’ abomasums. The ghrelin, obestatin and leptin immunohistochemical staining methods were used, which were applied to all of the sections following the routine histological process. Results: The immunoreactivity of ghrelin, obestatin and leptin was found to be different in all parts of the abomasum. Immunoreactivity was found to be dispersed settlement the abomasum in the cardia region. According to the mean H score, it was determined that ghrelin, obestatin and leptin were intensely expressed in the cardia, pilorus and fundus regions. Conclusion: The appetite hormones (ghrelin, obastatin and leptin) were expressed at different concentrations from the cardia, fundus and pilorus regions in the abomazum, suggesting that these hormones perform similar functions in hair goats considering their physiological effects on feed intake. More research is needed to about use of animal nutrition of ghrelin, obestatin and leptin hormones.

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