Tavşanların tendon yangılarının sağaltımında steroid ve trombositten zengin plazma (TZP) uygulamalarının karşılaştırmalı araştırılması

Amaç: Bu çalışmada, tavşanlarda kollajenaz (Kollajenaz Tip I) ile oluşturulmuş aşil tendinopatilerinde trombositten zengin plazma (PRP) ve MPA (metilprednizolon asetat) enjeksiyonlarının terapötik etkilerini histopatolojik olarak değerlendirmeyi amaçlamıştır. Gereç ve Yöntem: Aşil tendinopatisi, 23 tavşanın sağ ve sol aşil tendonlarının proksimal ve distal bölgelerine intratendinöz tip I kollajenaz enjeksiyonu ile oluşturuldu. Kolajenaz enjeksiyonundan bir hafta sonra, sağ aşil tendonlarının distaline PRP ve sol aşil tendonlarının distaline MPA enjekte edildi. Tüm aşil tendonlarının proksimal kısımlarına %0,9 serum fizyolojik enjekte edildi. Tavşanlar, terapötik enjeksiyonlardan 3, 6 ve 8 hafta sonunda ötenazi edildi. Tendonların histolojik dejenerasyon derecesi Modifiye Movin skorlamasına göre yapıldı. Bulgular: Tedavi grupları ile % 0,9’luk serum fizyolojik enjekte edilen grup arasında incelenen tüm dönemlerde istatistiksel açıdan önemli farklılıklar tespit (p

Comparison of steroid and platelet-rich plasma (PRP) applications in the treatment of collagenase induced tendinopathy in rabbit

Aim: The aim of this study was to evaluate histopathologically the therapeutic effects of platelet-rich plasma (PRP) and MPA (methylprednisolone acetate) injections in rabbits achill tendinopathies induced by collagenase (Collagenase Type I) Materials and Methods: Achilles tendinopathy was formed with intrathendinous injections of type I collagenase into the proximal and distal regions of the right and left achilles tendons of 23 rabbits. PRP was injected distal to the right Achilles tendons and MPA to the distal of the left Achilles tendons. A 0,9% saline solution was also injected into the proximal parts of all achilles tendons. Rabbits were euthanized 3, 6, and 8 weeks after the therapeutic injections. The degree of histopathological degeneration of the tendons was made according to the Modified Movin scoring. Results: Statistically significant differences were detected between the treatment groups and the 0.9% saline injected group (p0.05) without tenocyte nuclei (p

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Addison O, Marcus RL, LaStayo P, Ryan AS, 2014. Intermuscular fat: a review of the consequences and causes. Int J Endocrinol, 1–11.
Ahmed IM, Lagopoulos M, McConnell P, Soames RW, et al., 1998. Blood supply of the Achilles tendon. J Orthop Res, 16(5), 591-596.
Arroll B, Goodyear-Smith F, 2004. Corticosteroid injections for osteoarthritis of the knee: meta-analysis. BMJ, 328(7444), 869.
Aström M, Rausing A, 1995. Chronic Achilles tendinopathy.
A survey of surgical and histopathologic findings. Clin Orthop Relat Res, 316, 151-164.
Chen J, Yu Q, Wu B, Lin Z, et al., 2011. Autologous tenocyte therapy for experimental Achilles tendinopathy in a rabbit model. Tissue Eng Part A, 17(15-16), 2037-2048.
Coombes BK, Bisset L, Brooks P, Khan A, et al., 2013. Effect of corticosteroid injection, physiotherapy, or both on clinical outcomes in patients with unilateral lateral epicondylalgia: a randomized controlled trial. JAMA, 309(5), 461-469.
Culling CFA, Allison RT, Barr WT, 1985a. Connective tissue. Eds: CFA Culling, RT Allison, WT Barr, In: Cellular Pathology Technique. 4th edn. London, Butterworths and Co Ltd, pp; 164-80.
Culling CFA, Allison RT, Barr WT, 1985b. Haematoxylin and its couterstains. Eds: CFA Culling, RT Allison, WT Barr, In: Cellular Pathology Technique, 4th edn. London, Butterworths and Co Ltd, pp; 155-63.
Dahlgren LA, 2007. Pathobiology of tendon and ligament injuries. Clin Tech Equine Pract, 6(3), 168-173.
De Cesar Netto C, Godoy-Santos AL, Pontin PA, Natalino RJM, et al., 2018. Novel animal model for Achilles tendinopathy: Controlled experimental study of serial injections of collagenase in rabbits. PloS ONE 13(2), e0192769.
Faisal T, Qamar K, Asjid R, Umbreen F, et al., 2019. Effect of Autologous Platelet-rich Plasma on Appearance of Tenocytes at Injured Achilles Tendon Entheses in Rabbits. Jcpsp-J Coll Physıcı, 29(11), 1029-1033.
Fenwick SA, Hazleman BL, Riley GP, 2002. The vasculatureand its role in the damaged and healing tendon. Arthrıtıs Res Ther, 4(4), 1-9.
González JC, López C, Álvarez ME, Pérez JE, et al., 2016. Autologous leukocyte-reduced platelet-rich plasma therapy for Achilles tendinopathy induced by collagenase in a rabbit model. Sci Rep, 6, 19623.
Hoffmann A, Mamisch N, Buck FM, Espinosa N, et al., 2011.
Oedema and fatty degeneration of the soleus and gastrocnemius muscles on MR images in patients with Achilles tendon abnormalities. Eur Radıol, 21(9), 1996-2003.
Hooley CJ, Cohen RE, 1979. A model for the creep behaviour of tendon. Int J Biol Macromol, 1(3), 123-132.
Hugate R, Pennypacker J, Saunders M, Juliano P, 2004. The effects of intratendinous and retrocalcaneal intrabursal injections of corticosteroid on the biomechanical properties of rabbit Achilles tendons. JBJS, 86(4), 794-801.
Kannus P, 2000. Structure of the tendon connective tissue. Scand J Med Sci Sports, 10(6), 312-320.
Khan KM, Cook JL, Bonar F, Harcourt P, et al., 1999. Histopathology of common tendinopathies. Sports Med, 27(6), 393-408.
Klein MB, Yalamanchi N, Pham H, 2002. Flexor tendon healing in vitro: effects of TGF-β on tendon cell collagen production. J Hand Surg-Am, 27(4), 615-620.
Luscombe KL, Sharma P, Maffulli N, 2003. Achilles tendinopathy. Trauma, 5(4), 215-225.
Nichols AW, 2005. Complications associated with the use of corticosteroids in the treatment of athletic injuries. Clın J Sport Med, 15(5), E370.
Maffulli N, Ewen SW, Waterston SW, Reaper J, et al., 2000. Tenocytes from ruptured and tendinopathic Achilles tendons produce greater quantities of type III collagen than tenocytes from normal Achilles tendons: an in vitro model of human tendon healing. Am J Sports Med, 28(4), 499-505.
Marx RE, 2001. Platelet-rich plasma (PRP): what is PRP and what is not PRP? Implant Dent, 10(4), 225-228.
Millar NL, Hueber AJ, Reilly JH, Xu Y, et al., 2010. Inflammation is present in early human tendinopathy. Am J Sports Med, 38(10), 2085-2091.
Minkwitz S, Schmock A, Kurtoglu A, Tsitsilonis S, et al., 2017.
Time-dependent alterations of MMPs, TIMPs and tendon structure in human achilles tendons after acute rupture. İntJ Mol Sci, 18(10), 2199.
Movin T, Gad A, Reinholt FP, Rolf C, 1997. Tendon pathology in long-standing achillodynia: biopsy findings in 40 patients. Acta Orthop Scand, 68(2), 170-175.
Perucca Orfei C, Lovati AB, Viganò M, Stanco D, et al., 2016.
Dose-related and time-dependent development of collagenase-induced tendinopathy in rats. PloS ONE 11(8), e0161590.
Sànchez-Ibàñez JM, Fernández ME, Monllau JC, Alonso-Díez A, et al., 2015. New treatments for degenerative tendinopathy, focused on the región-specific of the tendón. Rheumatology (Sunnyvale), 5(173), 2161-1149.
Saxena K, 1988. Death from potassium chloride overdose. Postgrad Med, 84(1), 97-102.
Scutt N, Rolf CG, Scutt A, 2006. Glucocorticoids inhibit tenocyte proliferation and Tendon progenitor cell recruitment. J Orthop Res, 24(2), 173-182.
Smidt N, Assendelft WJ, Van der Windt DA, et al., 2002. Corticosteroid injections for lateral epicondylitis: a systematic review. Pain, 96, 23-40.
Takamura M, Yasuda T, Nakano A, Shima H, et al., 2017. The effect of platelet-rich plasma on Achilles tendon healing in a rabbit model. Acta Orthop Traumatol Turc, 51(1), 65-72.
Williams RB, Harkins LS, Hammond CJ, Wood JLN, 2001. Racehorse injuries, clinical problems and fatalities recorded on British racecourses from flat racing and National Hunt racing during 1996, 1997 and 1998. Equine Vet J, 33(5), 478-486.