Enver Yazar, Rahmi CANBAR, Muhittin Uslu, Öznur Tufan

Detomidinin embriyotoksik doz sınırının in-ovo model ile belirlenmesi

Amaç: Bu araştırmanın amacı detomidinin embriyotoksik doz sınırının fertil tavuk yumurtası kullanarak in-ovo model ile belirlenmesidir. Gereç ve Yöntem: Araştırmada 210 adet fertil yumurta 7 eşit (n: 30) gruba ayrılarak kuluçka makinasına optimum şartlarda yerleştirildi. Birinci gruba hiçbir uygulama yapılmadı. Diğer gruplardaki yumurtaların hava keselerine inkübasyonun yedinci günü, sırası ile serum fizyolojik, 10, 5, 2.5, 1.25 ve 0.625 mg/kg dozlarında detomidin uygulandı. İnkübasyonun 21. günü yumurtalar açılarak malformasyonlar, ölü embriyolar ve fertil olmayan yumurtalar belirlendi. Bulgular: 0.625, 1.25, 2.5, 5 ve 10 mg/kg dozlarında detomidin uygulanan gruplarda sırası ile 3, 2, 4, 6 ve 5 adet ölü embriyo belirlenirken, kontrol ve serum fizyolojik gruplarında ölü embriyo belirlenmedi. Kontrol ve 5 mg/ kg detomidin uygulanan gruplarda birer adet fertil olmayan yumurta belirlenirken, diğer gruplarda fertil olmayan yumurta gözlenmedi. 2.5, 5 ve 10 mg/kg dozlarda detomidin uygulanan gruplarda gözlenen ölüm oranları, kontrol ve serum fizyolojik gruplarından yüksek belirlendi (p

Determination of embryotoxic dose limit of detomidine with in-ovo model

Aim: The aim of this study is to determine the embryotoxic dose limit of detomidine with an in-ovo model using fertile hen’s eggs. Materials and Methods: 210 fertile eggs were divided into 7 equal (n: 30) groups and placed in the incubator under optimum conditions. No application was made to the first group. On the seventh day, normal saline solution, 10, 5, 2.5, 1.25, and 0.625 mg/kg doses of detomidine were administered to egg air-sacs in the other groups, respectively. On the 21st day, the eggs were opened and malformations, dead embryos and non-fertile eggs were determined. Results: While 3, 2, 4, 6 and 5 dead embryos were determined in 0.625, 1.25, 2.5, 5, and 10 mg/kg treated groups, respectively, no dead embryos were observed in the control and saline groups. While one non-fertile egg was determined in the control and 5 mg/kg detomidine groups, no nonfertile eggs were observed in the other groups. Mortality rates of detomidine group at doses of 2.5, 5 and 10 mg/kg were higher than the control and saline groups (p

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