HASAT ÖNCESİ 24–EPİBRASSİNOLİD (24–eBL) UYGULAMALARININ ALPHONSE LAVALLÉE VE HOROZ KARASI ÜZÜM ÇEŞİTLERİNDE ALFA, BETA, GAMA, DELTA TOKOFEROL BİRİKİMLERİ ÜZERİNE ETKİLERİ

Sofralık, kurutmalık ve şaraplık–şıralık olmak üzere farklı şekillerde değerlendirilen üzümün, besindeğerini ve antioksidan madde içeriğini zenginleştirmek amacıyla yetiştiricilikte çok farklı uygulamalaryapılabilmekte ve bu uygulamaların başında da büyümeyi düzenleyici madde kullanımları gelmektedir. Buaraştırma Alphonse Lavallée ve Horoz Karası üzüm çeşitlerine ait omcalara hasat öncesi yapılanbrassinosteroid uygulamalarının gerek besin değeri, gerekse antioksidan etkileri bakımından çok değerlibileşikler arasında yer alan α, β, γ ve δ–tokoferol birikimleri üzerine olan etkilerini belirlemek amacıylagerçekleştirilmiştir. Bu amaçla yeni nesil hormonlar arasında yer alan brassinosteroidlerin bir analoğu olan24–eBL, omcalara dört farklı konsantrasyon (0.2, 0.4, 0.6 ve 0.8 ppm) ve üç farklı uygulama dönemini (bendüşme dönemi; tane tutumundan 7 gün sonra +ben düşme dönemi; ile tane tutumundan 7 gün sonra+bendüşme dönemi+ben düşmeden 30 gün sonra) içeren kontrolle birlikte 13 uygulama şeklinde yapılmıştır. İkiyıl süreyle yapılan uygulamalar sonucunda en yüksek α, γ ve δ–tokoferol miktarlarının Horoz Karasıomcalarına ben düşme döneminde yapılan 0.8 ppm 24–eBL uygulamasından elde edildiği tespit edilmiştir.En yüksek β–tokoferol miktarının ise 1. yıl Alphonse Lavallée üzüm çeşidinde tane tutumundan 7 günsonra+ben düşme döneminde yapılan 0.4 ppm 24–eBL uygulamasından, 2. yıl ise Horoz Karası üzümçeşidinde ise ben düşme döneminde yapılan 0.6 ppm 24–eBL uygulamasından elde edildiği belirlenmiştir.

THE EFFECTS OF PREHARVEST 24–EPIBRASSINOLID (24–eBL) APPLICATIONS ON THE ACCUMULATION OF ALPHA, BETA, GAMMA, DELTA TOCOPHEROLS IN ALPHONSE LAVALLÉE AND HOROZ KARASI GRAPE CULTIVARS

Many different applications can be made in cultivation in order to enrich the nutrient and antioxidant contents of grapes evaluated in different forms such as table, raisin, wine and must. The most important treatments in grape growing for this purpose are the uses of plant growth regulators. This study was carried out to determine the effects of preharvest brassinosteroid applications on the accumulation of α, β, γ and δ– tocopherol, among the most valuable compounds in terms of nutrient value and antioxidant effects, in Alphonse Lavallée and Horoz Karası grape cultivars. For this purpose, 13 different treatments including control, four different concentrations (0.2, 0.4, 0.6 and 0.8 ppm) of 24–eBL as an analogue of brassinosteroids among the new generation of hormones, and three different application periods (veraison, 7 days after berry set+veraison, 7 days after berry set+veraison+30 days after veraison) were applied to vines. As a result of the applications performed for two years, the highest amounts of α, γ and δ–tocopherol were obtained from Horoz Karası applied with 0.8 ppm of 24–eBL at veraison. The highest amount of β– tocopherol was found in Alphonse Lavallée applied with 0.4 ppm 24–eBL at 7 days after berry set+veraison and in Horoz Karası applied with 0.6 ppm 24–eBL at veraison.

___

  • Bajguz, A. and S. Hayat, 2009. Effects of brassinosteroids on the plant responses to environmental stresses. Plant Physiology and Biochemistry. 47(1):1–8.
  • Biesaga–Koscielniak, J., Dziurka, M., Ostrowska, A., Mirek, M., Koscielniak, J. and A. Janeczko, 2014. Brassinosteroid Improves Content of Antioxidants in Seeds of Selected Leguminous Plants. Australian Journal of Crop Science. 8(3):378–388.
  • Choudhury, D., Das, A., Bhattacharya, A., and G. Chakrabarti, 2010. Aqueous Extract of Ginger Shows Antiproliferative Activity through Disruption of Microtubule Network of Cancer Cells. Food and Chemical Toxicology, 48(10):2872–2880.
  • Clouse, S.D. and J.M. Sasse, 1998. Brassinosteroids: Essential Regulators of Plant Growth and Development. Annual Review of Plant Physiology and Plant Molecular Biology, 49:427–451.
  • De Camargo, A.C., Regitano–D’Arce, M. A.B., Biasoto, A.C.T. and F. Shahidi, 2014. Low Molecular Weight Phenolics of Grape Juice and Winemaking Byproducts: Antioxidant Activities and Inhibition of Oxidation of Human Low–Density Lipoprotein Cholesterol and DNA Strand Breakage. Journal of Agricultural and Food Chemistry. 62(50):12159–12171.
  • Farooq, M., A. Wahid and S.M.A. Basra, 2009. Improving Water Relations and Gas Exchange with Brassinosteroids in Rice under Drought Stress. Journal of Agronomy and Crop Science. 195(4):262–269.
  • Farooq, M., Wahid, A., Lee, D.J., Cheema, S.A. and T. Aziz, 2010. Drought Stress: Comparative Time Course Action of the Foliar Applied Glycinebetaine, Salicylic Acid, Nitrous Oxide, Brassinosteroids and Spermine in Improving Drought Resistance of Rice. Journal of Agronomy and Crop Science 196(5):336–345.
  • Göktürk Baydar, N., 2006. Organic Acids, Tocopherols and Phenolic Compositions of Some Turkish Grape Cultivars. Chemistry of Natural Compounds. 42(2):156–159.
  • Göktürk Baydar, N. and M. Akkurt, 2001. Oil Content and Oil Quality Properties of Some Grape Seeds. Turkish Journal of Agricultural and Forestry. 25:163–168.
  • Göktürk Baydar, N. and G. Özkan, 2006. Tocopherol Contents of Some Turkish Wine By–Products. European Food Research and Technology 223(2):290–293.
  • Hayat, S., Yadav, S., Ali, B. and A. Ahmad, 2010. Interactive Effect of Nitric Oxide and Brassinosteroids on Photosynthesis and the Antioxidant System of Lycopersicon Esculentum. Russian Journal of Plant Physiology. 57(2):212–221.
  • Janeczko, A., J. Biesaga–Kościelniak, and M. Dziurka, 2009. 24–Epibrassinolide Modifies Seed Composition in Soybean, Oilseed Rape and Wheat. Seed Science and Technology. 37(3):625–639.
  • Kamal Eldin, A. and L.A. Appelqvist, 1996. The Chemistry and Antioxidant Properties of Tocopherols and Tocotrienols. Lipids. 31:671–701.
  • Khripach, V.A., V.N. Zhabinski and N.B. Khripach, 2003. New Practical Aspects of Brassinosteroids and Results of Their Ten Year Agricultural Use in Russia and Belarus. In Brassinosteroids: Bioactivity and crop productivity. Editors: Hayat, S., Ahmad, A. Netherlands: Springer.
  • Khripach, V., V. Zhabinskii and A.D. Groot, 2000. Twenty Years of Brassinosteroids: Steroidal Plant Hormones Warrant Better Crops for the 21. Century. Annals of Botany 86(3):441–447.
  • Kushi, L.H., Folsom, A.R., Prineas, R.J., Mink, P.J., Wu, Y. and R.M. Bostick, 1996. Dietary Antioxidant Vitamins and Death from Coronary Heart Disease in Postmenopausal Women. The New England J. of Medicine 334:1156–1162.
  • Malikova, J., J. Swaczynova, Z. Kolar and M. Strnad, 2008. Anticancer and Antiproliferative Activity of Natural Brassinosteroids. Phytochemistry 69(2): 418–426.
  • Nemhauser, J.L. and J. Chory, 2004. BRing it on: New Insights into the Mechanism of Brassinosteroid Action. Journal of Experimental Botany. 55(395):265–270.
  • Raghu, K. and S. Seeta Ram Rao, 2016. Effect of Brassinosteroids on Antioxidants Content and Radical Scavenging Activity of Tinospora cordifolia (Willd.) Miers ex Hook. F & Thoms. Journal of Medicinal Plants Studies. 4(5):117–121.
  • Sun, S., Kadouh, H.C., Zhu, W. and K. Zhou, 2016. Bioactivity–Guided Isolation and Purification of A–Glucosidase Inhibitor, 6–O–D–glycosides, from Tinta Cão Grape Pomace. Journal of Functional Foods. 23:573–579.
  • Szarka, A., B. Tomasskovics and G. Bánhegyi, 2012. The Ascorbate– glutathione–α–tocopherol Triad in Abiotic Stress Response. International Journal of Molecular Sciences. 13(4):4458–4483.
  • Xia, X.J., Huang, Y.Y., Wang, L., Huang, L.F., Yu, Y.L., Zhou, Y.H. and J.Q. Yu, 2006. Pesticides induced depression of photosynthesis was alleviated by 24– epibrassinolide pretreatment in Cucumis sativus L. Pesticide Biochemistry and Physiology. 86:42–48.
Bahçe-Cover
  • ISSN: 1300-8943
  • Yayın Aralığı: Yılda 2 Sayı
  • Başlangıç: 1968
  • Yayıncı: Atatürk Bahçe Kültürleri Merkez Araştırma Enstitüsü