INULA PEACOCKIANA (AITCH. & HEMSL.) KOROVIN'NIN YAPRAK ANATOMİSİ

Türkiye'de "uzunandızotu" olarak adlandırılan Inula peacockiana, 2 m'ye kadar boylanabilen, sarı çiçekli, güçlü, çok yıllık otsu bir bitkidir. Asteraceae familyasına ait tıbbi değeri olan bir bitkidir. Tıbbi bitkilerin güvenli kullanımı doğru teşhislerine bağlıdır. Yaprakların anatomik özellikleri, bitkilerin doğru teşhis edilmesinde önemlidir. Bu çalışmada I. peacockiana'nın taban yaprağı, yaprak sapı ve gövde yaprağının anatomik özellikleri incelenmiştir. Gereç ve Yöntem: Bitki materyalleri Van'dan (Türkiye) toplanmıştır. VANF Herbaryumuna bir örnek kaydedilmiştir. Anatomik çalışmalar için numuneler %70 alkolde korunmuştur. Enine ve yüzey kesitler bir jilet yardımıyla el ile alınmıştır. Mikroskobik incelemelerde kloralhidrat solüsyonu kullanılmıştır. Sonuç ve Tartışma: Taban ve gövde yaprakları dorsiventraldir, örtü ve salgı tüyleri içerir. Her iki yaprağın orta damarı dışa doğru çıkıntı yapmıştır. Taban yapraktan farklı olarak, gövde yaprağının üst yüzeyinde daha fazla salgı tüyü, alt yüzeyinde ise örtü tüyleri bulunur. Her iki yaprakta da üst ve alt epidermada ranunculaceae tipi stomalar gözlenmiştir. Basit yay şeklindeki taban yaprak sapının enine kesiti, parankimatik hücrelere gömülü çok sayıda ayrı iletim demet içerir. Epiderma çok sayıda örtü ve salgı tüyleri taşır.

LEAF ANATOMY OF INULA PEACOCKIANA (AITCH. & HEMSL.) KOROVIN

Objective: Inula peacockiana, which is called "uzunandızotu" in Turkey, is a stout perennial herbaceous plant with yellow flowers, grow up to 2 m. It is a plant belonging to the Asteraceae family, which has medicinal value. The safe use of medicinal plants depends on their correct diagnosis. The anatomical features of the leaves are valuable in diagnosing plants correctly. In this study, the anatomical features of the basal leaf, petiole and cauline leaf of I. peacockiana were investigated. Material and Method: Plant materials were collected from Van (Turkey). A voucher specimen was deposited in the VANF Herbarium. The samples for anatomical studies were preserved in 70% alcohol. The transverse and surface sections were cutted by hand with razor blade in microscopic preparation form. The chloral hydrate solution was used in microscopic examinations. Result and Discussion: The basal and cauline leaves are dorsiventral and contain cover and glandular hairs. The main vein protrudes outward in the midrib of the both leaves. Different the basal leaf, the cauline leaf contains more glandular hairs on the upper surface and cover hairs on the lower surface. In both leaves, ranunculaceae type stomata were observed in the upper and lower epidermis. The simple arc-shaped petiole includes of numerous separate bundles embedded in the parenchymatous cells. The epidermal layer consist of numerous cover and glandular hairs.

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1. Plants of the World Online Web site. (2022). Retrieved January 23, 2022, from http://www.plantsoftheworldonline.org
2. Qi, J.L., Fu, Y., Shi, X.W., Wu, Y.B., Wang, Y.Z., Zhang, D.Q., Shi, Q.W. (2008). Sesquiterpene lactones and their anti-tumor activity from the flowers of Inula britannica. Letters in Drug Design & Discovery, 5(7), 433-436. [CrossRef]
3. Chin, Y.W., Yoon, K.D., Kim, J. (2009). Cytotoxic anticancer candidates from terrestrial plants. Anti-Cancer Agents in Medicinal Chemistry, 9(8), 913-942. [CrossRef]
4. Zhang, T., Xiao, W., Gong, T., Yang, Y., Chen, R.Y., Yu, D.Q. (2010). Two new eudesmanolides from Inula racemosa. Journal of Asian natural products research, 12(9), 788-792. [CrossRef]
5. Cheng, X.R., Li, W.W., Ren, J., Zeng, Q., Zhang, S.D., Shen, Y.H., Yan, S.-K., Ye, J., Jin, H.-Z., Zhang, W.D. (2012). Sesquiterpene lactones from Inula hookeri. Planta Medica, 78(05), 465-471. [CrossRef]
6. Gökbulut, A., Kaiser, M., Brun, R., Sarer, E., Schmidt, T.J. (2012). 9β-hydroxyparthenolide esters from Inula montbretiana and their antiprotozoal activity. Planta Medica, 78(03), 225-229. [CrossRef]
7. Stojanović-Radić, Z., Čomić, L., Radulović, N., Blagojević, P., Denić, M., Miltojević, A., Rajković, J., Mihajilov-Krstev, T. (2012). Antistaphylococcal activity of Inula helenium L. root essential oil: eudesmane sesquiterpene lactones induce cell membrane damage. European journal of clinical microbiology & infectious diseases, 31(6), 1015-1025. [CrossRef]
8. Talib, W.H., Zarga, M.H.A., Mahasneh, A.M. (2012). Antiproliferative, antimicrobial and apoptosis inducing effects of compounds isolated from Inula viscosa. Molecules, 17(3), 3291-3303. [CrossRef]
9. Seca, A.M., Grigore, A., Pinto, D.C., Silva, A.M. (2014). The genus Inula and their metabolites: from ethnopharmacological to medicinal uses. Journal of Ethnopharmacology, 154(2), 286-310. [CrossRef]
10. Zeggwagh, N.A., Ouahidi, M.L., Lemhadri, A., Eddouks, M. (2006). Study of hypoglycaemic and hypolipidemic effects of Inula viscosa L. aqueous extract in normal and diabetic rats. Journal of Ethnopharmacology, 108(2), 223-227. [CrossRef]
11. Kaileh, M., Berghe, W.V., Boone, E., Essawi, T., Haegeman, G. (2007). Screening of indigenous Palestinian medicinal plants for potential anti-inflammatory and cytotoxic activity. Journal of Ethnopharmacology, 113(3), 510-516. [CrossRef]
12. Oskay, M., Oskay, D., Kalyoncu, F. (2009). Activity of some plant extracts against multi-drug resistant human pathogens. Iranian Journal of Pharmaceutical Research, 8, 293-300.
13. Kattouf, J., Belmoukhtar, M., Harnafi, H., Mekhfi, H., Ziyyat, A., Aziz, M., Bnouham, M., Legssyer, A. (2009). Antihypertensive effect of an aqueous extract of Inula viscosa leaves. Phytothérapie, 7(6), 309-312. [CrossRef]
14. Celik, T.A., Aslanturk, O.S. (2010). Evaluation of cytotoxicity and genotoxicity of Inula viscosa leaf extracts with Allium. Journal of Biomedicine and Biotechnology, 189252. [CrossRef]
15. Lee, N.H., Hong, J.I., Kim, J.Y., Chiang, M.H. (2009). Antioxidant properties and protective effects of Inula britannica var. chinensis regel on oxidative stress-induced neuronal cell damage. Korean Journal of Food Science and Technology, 41(1), 87-92.
16. Wang, J., Wang, N., Yao, X., Ishii, R., Kitanaka, S. (2006). Inhibitory activity of Chinese herbal medicines toward histamine release from mast cells and nitric oxide production by macrophage-like cell line, RAW 264.7. Journal of natural medicines, 60(1), 73-77. [CrossRef]
17. Shan, J.J., Yang, M., Ren, J.W. (2006). Anti-diabetic and hypolipidemic effects of aqueous-extract from the flower of Inula japonica in alloxan-induced diabetic mice. Biological and Pharmaceutical Bulletin, 29(3), 455-459. [CrossRef]
18. Somesh, T., Goel, K.K., Nidhi, G. (2011). Antiinflammatory activity of Inula cuspidata leaf extract. Asian Journal of Chemistry, 23(2), 943-944.
19. Gebre-Mariam, T., Neubert, R., Schmidt, P.C., Wutzler, P., Schmidtke, M. (2006). Antiviral activities of some Ethiopian medicinal plants used for the treatment of dermatological disorders. Journal of Ethnopharmacology, 104(1-2), 182-187. [CrossRef]
20. Pal, H.C., Sehar, I., Bhushan, S., Gupta, B.D., Saxena, A.K. (2010). Activation of caspases and poly (ADP-ribose) polymerase cleavage to induce apoptosis in leukemia HL-60 cells by Inula racemosa. Toxicology In Vitro, 24(6), 1599-1609. [CrossRef]
21. Ajani, H.B., Patel, H.P., Shah, G.B., Acharya, S.R., Shah, S.K. (2009). Evaluation of antidiabetic effect of methanolic extract of Inula racemosa root in rats. Pharmacologyonline, 3, 118-129.
22. Ojha, S., Nandave, M., Kumari, S., Arya, D.S. (2010). Cardioprotection by Inula racemosa Hook in experimental model of myocardial ischemic reperfusion injury. Indian Journal of Experimental Biology, 48, 918-924.
23. Guinoiseau, E., Luciani, A., Rossi, P.G., Quilichini, Y., Ternengo, S., Bradesi, P., Berti, L. (2010). Cellular effects induced by Inula graveolens and Santolina corsica essential oils on Staphylococcus aureus. European Journal of Clinical Microbiology & Infectious Diseases, 29(7), 873-879. [CrossRef]
24. Attard, E., Cuschieri, A. (2009). In vitro immunomodulatory activity of various extracts of Maltese plants from the Asteraceae family. Journal of Medicinal Plants Research, 3(6), 457-461.
25. Khan, A.L., Hussain, J., Hamayun, M., Gilani, S.A., Kim, Y.H., Rehman, S.U., Watanabe, K.N., Lee, I.J. (2010). Elemental allelopathy and antifungal activities of Inula falconeri from Himalaya Pakistan. Acta Agriculturae Scandinavica, Section B-Soil & Plant Science, 60(6), 552-559. [CrossRef]
26. Güner, A., Aslan, S., Ekim, T., Vural, M., Babaç, M.T. (2012). Türkiye Bitkileri Listesi (Damarlı Bitkiler). Nezahat Gökyigit Botanik Bahçesi Yayınları, Flora Dizisi I.
27. Davis, P.H. (1975). Flora of Turkey and the East Aegean Islands, Edinburgh University Press, Edinburgh.
28. Hürkul, M.M., Yayla, Ş. (2021). Leaf anatomy of Quercus macranthera subsp. syspirensis (K. Koch) Menitsky. Biological Diversity and Conservation, 14(3), 405-410. [CrossRef]
29. Metcalfe, C.R., Chalk, L. (1965). Anatomy of Dicotyledones, Clarendon Press, Oxford.