Yasin DARI, Süleyman YUR, Gülmira ÖZEK, Ülkü Dilek UYSAL, Temel ÖZEK

SİYAH VE BEYAZ CHIA TOHUMLARININ FARKLI YÖNTEMLERLE ELDE EDİLEN EKSTRELERİNİN BİYOLOJİK AKTİVİTE TAYİNİ

Amaç: Siyah ve beyaz chia tohumlarının biyolojik aktivitelerinin farklı esktraksiyon teknikleriyle elde edilen ekstrelerde tayinlerinin yapılması ve karşılaştırılması.Gereç ve Yöntem: Farklı tekniklerle elde edilen ekstrelerin biyolojik aktivite çalışmaları, DPPH radikali süpürme potansiyeli testi, TEAC testi ve ORAC testi uygulanarak tayin edilmiştir. Ayrıca metanollü ekstre numunelerinde toplam fenol ve flavonoit miktar tayinleri yapılmıştır.Sonuç ve Tartışma: Bu çalışmada fenol miktarı siyah chia tohumu için 9,86 mg GAE/g, beyaz chia tohumu için ise 12,69 mg GAE/g olarak belirlenmiş olup, toplam flavonoid miktarı ise siyah chia tohumu için 0,098 mg RE/mL ve beyaz chia tohumu için 0,099 mg RE/mL olarak belirlenmiştir. Ayrıca yapılan antioksidan aktivite testleri göz önüne alındığında, TEAC testi için en yüksek antioksidan aktivite, soğuk sıkım yöntemiyle elde edilen hem siyah hem de beyaz tohum ekstresinde, DPPH için en yüksek aktivite, soğuk sıkım tekniğiyle elde edilen beyaz tohum ekstresinde, ORAC testi için en yüksek aktivite ise Folch tekniğiyle elde edilen beyaz tohum ekstresinde görülmüştür.

Anahtar Kelimeler:

Biyolojik aktivite, chia, DPPH, ORAC, TEAC

BIOLOGICAL ACTIVITY DETERMINATION OF BLACK AND WHITE CHIA SEED EXTRACTS OBTAINED BY DIFFERENT EXTRACTION METHODS

Objective: Determination and comparison of the biological activities of black and white chia seeds in extracts obtained by different extraction techniques.

Keywords:

Biological activity, chia, DPPH, ORAC, TEAC,

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