Gizem Rüya TOPAL, Merve Eylül KIYMACI, Yalçın ÖZKAN

ENTEROKOK ENFEKSİYONLARINDA KULLANILMAK ÜZERE VANCOMİSİN İÇEREN PLGA NANOPARTİKÜLLERİN HAZIRLANMASI VE KARAKTERİZASYONU

Amaç: Bu çalışmada enterokok enfeksiyonlarında parenteral olarak kullanılabilecek Vankomisin yüklü poli(laktik asit-ko-glikolik asit) (PLGA) nanopartikülleri hazırlanması amaçlanmıştır.

Anahtar Kelimeler:

Antibakteriyel aktivite, Enterococcus faecalis, Nanopartikül, PLGA, Vankomisin

PREPARATION AND IN VITRO CHARACTERIZATION OF VANCOMYCIN LOADED PLGA NANOPARTICLES FOR THE TREATMENT OF ENTEROCOCCUS FAECALIS INFECTIONS

Objective: In this study, it was aimed to prepare Vancomycin loaded poly(lactic acid-co-glycolic acid) (PLGA) nanoparticles that can be used parenterally in enterococcal infections.

Keywords:

Antibacterial activity, Enterococcus faecalis, Nanoparticles, PLGA, Vancomycin,

PDF

___

Yıldırım, M. (2022). Enterokoklar ve Enterokoklarla Gelisen Infeksiyonlar. 9.
Said MS, Tirthani E, Lesho E. Enterococcus Infections. (2021). In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2021
Tünger, Ö. (2012). Vankomisine dirençli enterokok infeksiyonlarının tedavisinde eski ve yeni tedavi seçenekleri. ANKEM Dergisi, 26(4), 215-227.
Soppimath, K. S., Aminabhavi, T. M., Kulkarni, A. R., & Rudzinski, W. E. (2001). Biodegradable polymeric nanoparticles as drug delivery devices. Journal of Controlled Release, 70(1), 1-20.
Anderson, J. M., & Shive, M. S. (1997). Biodegradation and biocompatibility of PLA and PLGA microspheres. Advanced Drug Delivery Reviews, 28(1), 5-24.
Valizadeh, H., Mohammadi, G., R.Ehyaei, Milani, M., Azhdarzadeh, M., Zakeri-Milani, P., & Lotfipour, F. (2012). Antibacterial activity of clarithromycin loaded PLGA nanoparticles. Pharmazie, 67, 63-68.
Verderio, P., Bonetti, P., Colombo, M., Pandolfi, L., & Prosperi, D. (2013). Intracellular drug release from curcumin-loaded PLGA nanoparticles induces G2/M block in breast cancer cells. Biomacromolecules, 14(3), 672-682.
Lotfipour, F., Abdollahi, S., Jelvehgari, M., Valizadeh, H., Hassan, M., & Milani, M. (2014). Study of antimicrobial effects of vancomycin loaded PLGA nanoparticles against enterococcus clinical isolates. Drug Res (Stuttg), 64(7), 348-352.
Anwer, M. K., Al-Mansoor, M. A., Jamil, S., Al-Shdefat, R., Ansari, M. N., & Shakeel, F. (2016). Development and evaluation of PLGA polymer based nanoparticles of quercetin. Int J Biol Macromol, 92, 213-219.
Esim, O., Bakirhan, N. K., Sarper, M., Savaser, A., Ozkan, S. A., & Ozkan, Y. (2020). Influence of emulsifiers on the formation and in vitro anticancer activity of epirubicin loaded PLGA nanoparticles. Journal of Drug Delivery Science and Technology, 60, 102027.
Zakeri-Milani, P., Loveymi, B. D., Jelvehgari, M., & Valizadeh, H. (2013). The characteristics and improved intestinal permeability of vancomycin PLGA-nanoparticles as colloidal drug delivery system. Colloids Surf B Biointerfaces, 103, 174-181.
Topal, G. R., Devrim, B., Eryilmaz, M., & Bozkir, A. (2018). Design of ciprofloxacin-loaded nano-and microcomposite particles for dry powder inhaler formulations: preparation, in vitro characterisation, and antimicrobial efficacy. J Microencapsul, 35(6), 533-547.
Simon, A., Moreira, M. L. A., Costa, I., de Sousa, V. P., Rodrigues, C. R., da Rocha, E. L. L. M. T., . . . Cabral, L. M. (2020). Vancomycin-loaded nanoparticles against vancomycin intermediate and methicillin resistant Staphylococcus aureus strains. Nanotechnology, 31(37), 375101.
EUCAST Web site. (2021). European Committee on Antimicrobial Susceptibility Testing. https://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Breakpoint_tables/v_11.0_Breakpoint_Tables.pdf Accessed date: 9.12.2021.
Tewes, F., Munnier, E., Antoon, B., Ngaboni Okassa, L., Cohen-Jonathan, S., Marchais, H., . . . Chourpa, I. (2007). Comparative study of doxorubicin-loaded poly(lactide-co-glycolide) nanoparticles prepared by single and double emulsion methods. Eur J Pharm Biopharm, 66(3), 488-492.
Sabaeifard, P., Abdi-Ali, A., Soudi, M. R., Gamazo, C., & Irache, J. M. (2016). Amikacin loaded PLGA nanoparticles against Pseudomonas aeruginosa. Eur J Pharm Sci, 93, 392-398.
Seju, U., Kumar, A., & Sawant, K. K. (2011). Development and evaluation of olanzapine-loaded PLGA nanoparticles for nose-to-brain delivery: In vitro and in vivo studies. Acta Biomaterialia, 7(12), 4169-4176.
Quintanar-Guerrero, D., Fessi, H., Allémann, E., & Doelker, E. (1996). Influence of stabilizing agents and preparative variables on the formation of poly(d,l-lactic acid) nanoparticles by an emulsification-diffusion technique. International Journal of Pharmaceutics, 143(2), 133-141.
Mainardes, R. M., & Evangelista, R. C. (2005). PLGA nanoparticles containing praziquantel: effect of formulation variables on size distribution. International Journal of Pharmaceutics, 290(1), 137-144.
Bacanlı, M., Eşi̇m, Ö., Erdoğan, H., Sarper, M., Erdem, O., & Özkan, Y. (2021). Evaluation of cytotoxic and genotoxic effects of paclitaxel-loaded PLGA nanoparticles in neuroblastoma cells. Food and Chemical Toxicology, 154, 112323.
Tefas, L. R., Tomuţă, I., Achim, M., & Vlase, L. (2015). Development and optimization of quercetin-loaded PLGA nanoparticles by experimental design. Clujul medical (1957), 88(2), 214-223.
Elsewedy, H. S., Dhubiab, B. E. A., Mahdy, M. A., & Elnahas, H. M. (2020). Development, optimization, and evaluation of PEGylated brucine-loaded PLGA nanoparticles. Drug Delivery, 27(1), 1134-1146.
Murakami, H., Kobayashi, M., Takeuchi, H., & Kawashima, Y. (1999). Preparation of poly(DL-lactide-co-glycolide) nanoparticles by modified spontaneous emulsification solvent diffusion method. Int J Pharm, 187(2), 143-152.
Singh, G., Tanurajvir, K., Ravinder, K., & Kaur, A. (2014). Recent biomedical applications and patents on biodegradable polymer-PLGA. International Journal of Pharmacology and Pharmaceutical Sciences, 1, 30-42.
Sunanda Laxmi, P., Vidyavathi, M., & Venkata, S. K. R. (2021). DoE approach for development of localized controlled release microspheres of Vancomycin for treatment of septic arthritis. Future Journal of Pharmaceutical Sciences, 7(1).
Alonso, M. J., Losa, C., Calvo, P., & Vila-Jato, J. (1991). Approaches to improve the association of amikacin sulphate to poly(alkylcyanoacrylate) nanoparticles. International Journal of Pharmaceutics, 68(1), 69-76.
Abeylath, S. C., Turos, E., Dickey, S., & Lim, D. V. (2008). Glyconanobiotics: Novel carbohydrated nanoparticle antibiotics for MRSA and Bacillus anthracis. Bioorg Med Chem, 16(5), 2412-2418.