Reverse transkriptaz-polimeraz zincir reaksiyon (RT-PCR) yöntemi kullanılaak hıyar mozayik virüsü enfeksiyonunun belirlenmesi

Son yıllarda moleküler yöntemlerin kullanılması ile bitki virolojisi alanında çok önemli gelişmeler elde edilmiştir. Polimeraz zincir reaksiyonu (PCR) yöntemi, bitki virüslerinin teşhisinde, viral genlerin enfeksiyon sırasındaki rollerinin araştırılmasında en fazla kullanılan moleküler yöntemlerden birisidir. Bu çalışmada, Samsun ilinde hıyar üretim alanlarından toplanan örneklerden izole edilen Hıyar mozayik virüsü (CMV)' nün kapsid protein genini içeren bölge, reverse trasnskriptaz-polimeraz zincir reaksiyonu (RT-PCR) yöntemi kullanılarak belirlenmiştir.

Anahtar Kelimeler:

teknikler, analitik yöntemler, kapsid protein, hıyar mozaik kukumovirüsü, genler, nükleotit dizileri, bitki patojenleri, bitki virüsleri, polimeraz zincir reaksiyonu, tersine transkripsiyon

Determination of Cucumber mosaic virus infection by using reverse transcription-polymerase chain reaction (RT-PCR) method

In recent years, significant developments have been obtained by using molecular methods in plant virology. Polymerase chain reaction (PCR) has become one of the most common used molecular techniques in virus detection and determination of viral genes and their functions. In this study, the sequences involving capsid-protein gene of Cucumber mosaic virus (CMV) was amplified by using reverse transcription - polymerase chain reaction (RT-PCR).

Keywords:

techniques, analytical methods, coat proteins, cucumber mosaic cucumovirus, genes, nucleotide sequences, plant pathogens, plant viruses, polymerase chain reaction, reverse transcription,

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