Melike BARAN EKİNCİ, Aynur Gül KARAHAN ÇAKMAKÇI

Toprak Kökenli Miksobakteri İzolatlarından Alfa-Amilaz Enzimi Üretimi ve Saflaştırılması

Bu çalışmada farklı il (Antalya, Konya, Isparta) ve ilçelerden (Kulu, Şarkikaraağaç, Atabey) toplanan farklı habitatlara ait 28 toprak örneğinden 19 miksobakteri suşu izole edilmiştir. İzole edilen 19 miksobakteri arasında Myxococcus sp KK2’nin en yüksek α-amilaz aktivitesine sahip olduğu belirlenmiştir. Protein çöktürülmesi ile ilk uygulamada %40, 65 ve 80 amonyum sülfat konsantrasyonları, ikinci uygulamada ise %20, 40 ve 65 konsantrasyonları kullanılarak enzim saflığı artırılmıştır. Diyaliz ve jel filtrasyon yöntemleri kullanılarak ilk uygulamada elde edilen enzimin aktivitesi 883.27 U’ya ulaşırken enzim saflığı 28.35 kat artmıştır. İkinci uygulama ile ise enzim aktivitesi 1754.99 U olarak saptanmış ve enzim saflığı 810.24 kat artmıştır.

Anahtar Kelimeler:

Miksobakteriler, Myxococcus, Enzim saflaştırma

Alpha-Amylase Production and Purification from Myxobacteria Isolates of Soil Origin

In this study, 19 myxobacteria strains were isolated from 28 soil samples in the different habitats. They were collected from different provinces (Antalya, Konya and Isparta) and their districts (Kulu, Şarkikaraağaç and Atabey). Among the 19 myxobacteria isolated, Myxococcus sp KK2 was determined to have the highest α-amylase activity. With protein precipitation, enzyme purity was increased by using 40, 65 and 80% ammonium sulfate concentrations in the first application, then 20, 40 and 65% concentrations in the second application. By using dialysis and gel filtration methods, the activity of the enzyme obtained in the first application reached 883.27 U while enzyme purity increased 28.35 times, and enzyme activity was 1754.99 U after the second application while enzyme purity increased 810.24 times.

Keywords:

Myxobacteria, Enzyme purification, Myxococcus,

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