Phenolic Content of Some Dietetic Tea Products in Turkey

Türkiye'de satılan on diyet çay örneğinin (n=10) fenolik bileşik içeriği, yüksek performanslı sıvı kromatografisi (HPLC) sistemi ile belirlenmiştir. En çok bulunan fenolik bileşikler, gallik asit (ND-6.98 mg/100 g kuru numune), protokateşik asit (0.98-45.61 mg/100 g kuru numune), 4-hidroksibenzoik asit (ND-31.56 mg/100 g kuru numune), klorojenik asit (ND-0.73 mg/100 g kuru numune), kafeik asit (11.27-154.55 mg/100 g kuru numune), p-kumarik asit (ND-6.56 mg/100 g kuru numune), ferulik asit (ND-1.13 mg/100 g dw), ellajik asit (0.10-1.76 mg/100 g kuru numune), epikateşin (7.59290.07 mg/100 g kuru numune), rutin (ND-49.95 mg/100 g kuru numune) ve kuersetindir (1.00 mg/100 g kuru numune). Elde edilen sonuçlara göre, hassasiyet ve doğruluk iyi bulunmuştur. Kalibrasyon eğrileri, tüm bileşikler için iyi bir doğrusallık göstermiştir (r2> 0.999). Diyet çay ürünlerinde tespit limiti, 0.010-0.050 ?g/g arasında değişmiştir. Geri kazanımlar %86.45-97.35 aralığındadır. Bu çalışmada elde edilen sonuçların doğruluk ve tekrarlanabilirliklerinin iyi olduğu belirlenmiştir.

Türkiye'deki Bazı Diyet Çay Örneklerinin Fenolik Bileşik İçeriği

High performance liquid chromatography (HPLC) system was used to determine phenolic contents of dietetic tea samples (n=10) marketed in Turkey. The most abundant phenolic compounds were gallic acid (ND-6.98 mg/100 g dw), protocatechuic acid (0.98-45.61 mg/100 g dw), 4-hydroxybenzoic acid (ND-31.56 mg/100 g dw), chlorogenic acid (ND-0.73 mg/100 g dw), caffeic acid (11.27-154.55 mg/100 g dw), p-coumaric acid (ND-6.56 mg/100 g dw), ferulic acid (ND-1.13 mg/100 g dw), ellagic acid (0.10-1.76 mg/100 g dw), epicatechin (7.59-290.07 mg/100 g dw), rutin (ND-49.95 mg/100 g dw) and quercetin (1.15-10.50 mg/100 g dw). Based on the results of this study, sensitivity and accuracy were good. Calibration curves had a good linearity for all compounds (r2>0.999). The quantitation limit ranged 0.010 to 0.050 µg/g in dietetic tea products. Recoveries were in the range of 86.45-97.35%. Results had good accuracy and reproducibility.

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