Koşullandırılmış Kalem Grafit Elektrot Kullanılarak Vanilinin Voltammetrik Tayini

Bu çalışmada, bir elektrot malzemesi olarak kurşun kalem ucu kullanılarak ticari gıda ürünlerinde vanilin tayini için hassas bir voltammetrik yöntem önerilmiştir. Vanilinin, önceden oksitlenmiş grafit kalem elektrotu (pre-oxidized pencil graphite electrode, p-PGE) kullanılarak pH 8.0 Britton Robinson tampon çözeltisinde kaydedilen döngüsel voltammogramlarında, yaklaşık 520 mV’da (Ag/AgCl elektroduna karşı) vanilinin yükseltgenmesine atfedilen keskin bir pik gözlenmiştir. Daha sonra, vanilinin p-PGE’de yükseltgenmesine dayalı voltammetrik vanilin tayini, optimize koşullar altında diferansiyel puls voltammetri (DPV) tekniği kullanılarak gerçekleştirildi. DPV sonuçları, vanilinin yükseltgenmesine ait pik akımının, 0,16 µM (3Sb’ye dayanarak) gözlenebilme sınırı ile birlikte 0,5-10,0 µM vanilin derişim aralığında doğrusal olarak arttığını göstermektedir. Çalışmanın son aşamasında, bu voltammetrik yöntem vanilya şurubu örneklerinde vanilin tayinine uygulanmıştır. Elde edilen sonuçların numune şişesindeki etiketli değerler ile iyi bir uyum içinde olduğu tespit edilmiştir.

Anahtar Kelimeler:

Kalem grafit elektrot, Vanilin, Voltammetrik tayin, Diferansiyel puls voltammetri

Voltammetric Determination of Vanillin Using a Pretreated Pencil Graphite Electrode

In this study, a sensitive voltammetric method for the determination of vanillin in commercial food products was proposed using a pencil lead as an electrode material. In the cyclic voltammograms of vanillin recorded in pH 8.0 Britton Robinson buffer at the pre-oxidized pencil graphite electrode (p-PGE), a sharp peak was observed at about 520 mV (vs. Ag / AgCl electrode) attributed to the oxidation of vanillin. Subsequently, the voltammetric determination of vanillin based on its oxidation at p-PGE was carried out using the differential pulse voltammetry (DPV) technique under optimized conditions. DPV results showed that the oxidation peak current of vanillin increased linearly in the concentration range of 0.5 to 10.0 µM vanillin with a detection limit of 0.16 µM (based on 3Sb). In the final step, this voltammetric method was applied to the determination of vanillin in vanilla syrup samples. Results were in good agreement with the values indicated on the labels of samples.

Keywords:

Pencil graphite electrode, Vanillin, Voltammetric determination, Differential pulse voltammetry,

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