A.к. MAMYRBEKOVA, M.k. KASYMOVA, A.к. MAMYRBEKOVA, A.d. MAMİTOVA

STUDY OF BIOTECHNOLOGICAL METHOD OF PRODUCING PLANTS РETUNIA HYBRIDA L. RESISTANT TO HEAVY METALS

In work conditions for introduction in culture in vitro Рetunia hybrida L. are picked up and the culture is received callus. The degree of toxic action of ions of cadmium, zinc and lead is investigated, including at their joint presence for callus cultures and the whole plants, in particular, petunias hybrid. Influence of concentration of cadmium, lead and zinc on seeds of a plant, growth and survival rate callus cells is studied. It is shown, that accumulation of heavy metals in plants depends on features of a kind, properties of metal and conditions of environment.

Anahtar Kelimeler:

Рetunia hybrida L., cultivation, producer, callus, biotechnology, heavy metal

STUDY OF BIOTECHNOLOGICAL METHOD OF PRODUCING PLANTS РETUNIA HYBRIDA L. RESISTANT TO HEAVY METALS

Keywords:

Рetunia hybrida L., cultivation, producer, callus, biotechnology, heavy metal,

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1. Hinesly T.D., Alexander D.E., Redborg K.E., Liegler E.L. Differential accumulations of Cd and Zn by Corn Hybrids Grown on Soil Amended with Sewage Sludge. // Agronomy Journal, 2007. V. 74. P. 469-474.
2. Gladkov E.A., Gladkova O.V. Biotekhnologicheskie metody polucheniia rastenii, ustoichivykh k tiazhelym metallam // Biotekhnologiia, № 1, 2007.- S. 81-85.
3. Rasteniia v ekstremal'nykh usloviiakh mineral'nogo pitaniia /Pod red. N.V. Alekseevoi-Popovoi. - L.: Nauka, 2013.-178 s.
4. Gladkov E.A., Gladkova O.N., Gladkova O.V. Mir lekarstvennykh rastenii. -M.: Pas'va, 2004.- 64 s.
5. Ernst W. Physiology of metal resistance in plants // Proc. Int. Conf. On Heavy Metals in the environment.- Toronto, Canada. 2005.- Vol. 11.-P. 121-136.
6. Kitagishi K., Yamane I., Eds. Heavy Metal Pollution in Soil of Japan, Japan Science Society Press, Tokyo, 2011. 302 p.
7. McGrath S.P., Sidoli C.M., Baker A.J.M., Reeves R.D. Using plants to clean up heavy metal in soils.// 15 th World Congr. Soil Sci., Acapulco, July, 1994: Trans. Vol. 4a Commiss. 3 Symp.–Mexico, 2012. p. 362-363.
8. Valikhanova G.Zh., Rakhimbaev I.R., Karzhasova A.V., Bishimbaeva N.K. Metodicheskoe rukovodstvo k prakticheskim zaniatiiam po kul'ture tkanei rastenii. -Alma-Ata: KazGU, 1998. -96 s.
9. Gladkov E.A. Sravnitel'naia otsenka toksichnosti tiazhelykh metallov dlia kallusnykh kletok i tselykh rastenii. //Biotekhnologiia, № 3, 2006.- S. 79-82.
10. Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures // Physiol. plant.1998. V. 15. №13. P. 473–497.
11. Il'in V.B. Tiazhelye metally v sisteme pochva-rastenie. - Novosibirsk: Nauka, Sib.otdelenie, 1991.- 151 s.
12. Thurman D.A. Mechanisms of metal tolerance in higher plants. Applied Sci. Publ. 2011. V. 2. P. 239.