MEHMET KARACA, Cahit BABÜR, Bekir ÇELEBİ, HASAN ALTAN AKKAN, Mehmet TÜTÜNCÜ, İHSAN KELEŞ, BARIŞ ATALAY USLU, Selçuk KILIÇ

Investigation on the seroprevalence of toxoplasmosis, listeriosis and brucellosis in goats living in the region of Van,Turkey

Bu çalışma Van yöresi keçilerinde toxoplasmosis, listeriosis ve brucellosis seroprevalansını belirlemek için yapıldı. Toxoplasma gondii, Listeria monocytogenes ve Brucella melitensis’e karşı oluşan antikorları belirlemek için sırası ile Sabin Feldman Dye Testi (SFDT), Osebold Agglutination Testi (OAT) ve Micro Agglutination Testi (MAT) uygulandı. 98 serum örneğinin, %80.61 toxoplasmosis, %34.69 listeriosis and %6.12 brucellosis yönünden seropozitif olduğu tesbit edildi. Bu sonuçlar ile Van yöresi keçilerinde Toxoplasmosisin, listeria ve brucella’ya göre çok daha yaygın olduğu görüldü.

Anahtar Kelimeler:

Listeria, Brucella, Toxoplasma, Türkiye, keçi, seroprevalans, Doğu Anadolu Bölgesi

Van yöresi keçilerinde toxoplasmozis, listeriozis ve brucellozis'in seroprevalansı üzerine araştırmalar

This study was carried out on goat sera to determine the seroprevalence of listeriosis, toxoplasmosis and brucellosis in the province of Van. Sabin Feldman Dye Test (SFDT), Osebold Agglutination Test (OAT) and Micro Agglutination Test (MAT) were applied to determine antibodies developed against Toxoplasma gondii, Listeria monocytogenes and Brucella melitensis respectively. Out of the 98 sera, 80.61% toxoplasmosis, 34.69% listeriosis and 6.12% brucellosis were evaluated as positive. This results indicate that Toxoplasmosis is much more widespread than Listeriosis, and Brucellosis in goats living in the province of Van.

Keywords:

Listeria, Brucella, Toxoplasma, Turkey, goats, seroprevalence, East Anatolia Region,

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1. Al-Majali, A.M. (2005): Seroepidemiology of caprine Brucellosis in Jordan. Small Ruminant Research. 58; 13-18.
2. Aslan, V., Turgut, K., Kaya, O., Sevinç, M. (1991): Sıgırlarda Listeriosis Olgusu. Hayvancılık Arastırma Derg. 1 (1): 37-39
3. Baetz, A. L., Wesley, I.V. (1995): Detection of antilisteriolysin O in dairy cattle experimentally infected with Listeria monocytogenes. J Vet Diagn Invest. 7(1):82-6.
4. Barbuddhe, S.B., Chaudhari, S.P, Malik, S.V.S. (2002): The occurrence of pathogenic Listeria monocytogenes and antibodies against listeriolysin-O in buffaloes. J Vet Med B Infect Dis Vet Public Health. 49 (4): 181-4.
5. Barbuddhe, S.B., Malik, S.V.S., Bhilegaonkar, K.N., Gupta, L. K. (2000): Isolation of Listeria monocytogenes and anti-listeriolysin O detection in sheep and goats. Small Ruminant Research. 38; 151-154.
6. Baum, M., Zamir, O., Bergman-Rios, R., Katz, E., Beider, Z., Cohen, A., Banal, M. (1995): Comparative Evaluation of Microagglutination Test and Serum Agglutination Test as Supplementary Diagnostic Methods for Brucellosis. J. Clin Microbiol. 33: 2166-2170
7. Berche, P., K. A. Reich, M. Bonnicon, J. L. Beretti, C. Geoffroy, J. Raveneau, R. Cossart, J. L. Gaillard, P. Geslin, H. Kreis, and M. Veron, 1990: Detection of anti-listeriolysin O for serodiagnosis of human listeriosis. Lancet. 335, 624-627.
8. Boerlin, P., Boerlin-Petzold, F., Jemmi, T. (2003): Use of Listeriolysin O and Internalin A in a Seroepidemiological study of Listeriosis in Swiss Dairy Cows. J of Clinical Micro. 1055-1061.
9. Borde, G., Lowhar, G and Adesıyun A.A.(2006): Toxoplasma gondii and Chlamydophila abortus in caprine Abortions in Tobago: a sero-Epidemiological Study. J. Vet. Med. B. 53, 188-193.
10. A. M., and Commander, N. J. (2005): A review: Brucellosis- new aspects of an old disease. J. of Applied Microbiology. 98; 1270-1281.
11. Dean, A.G., Dean, J.G., Coulombier, D., Berendel, K.A., Smith, D.C., Burton, A.H, Dicker, R.C., Sullivan, K.M., Fagan, R.F., Arner, T.G. (1994): Epi-Info Version 6: A Word Processing Database and Statistics Program for Epidemiology on Microcomputers. Center for Disease Control and Prevention. Atlanta. Georgia. USA.
12. Donachie, W., Low, J.C. (1995): Ovine Listeriosis. The Veterinary Annual. 35, 304-312.
13. Erdogan, H.M, Gökçe, G., Gökçe, H., Kırmızıgül, A.H, Günes, V., Sural, E., Yılmaz, K. (1999): Kars yöresindeki sıgırlarda Listeria monocytogenes enfeksiyonlarının ELISA yöntemi ile arastırılması. Kafkas Ü. Vet. Fak. Derg. 5(1): 43-46.
14. Esendal, Ö.M., Yardımcı, H., Keskin, O., Altay, G. (2001): Sıgır, Koyun ve Keçi Brucellosis’inin Serolojik Tanısında Konvansiyonel Testler ve Coombs Testinin Kullanılıması. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 48: 97-102.
15. Farber, J. M., and. Peterkin, P. I (1991): Listeria monocytogenes, a food-borne pathogen. Microbiol. Rev. 55, 476-511.
16. Feldman, H.A and Lamb, G. A. (1966): A micromodification of the Toxoplasma Dye Test. J Parasitol; 52: 415.
17. Garin-Bastuji, B., Blasco, J. M. Marin, C., Albert, D. (2006): The diagnosis of brucellosis in sheep and goats, old and new tools. Small Ruminant Research. 62; 63-70.
18. Gasanov, U., Hughes, D., and Hansbro, M. P: (2005): Methods for the isolation and idendification of Listeria spp. and Listeria monocytogenes: a review. FEMS microbiology Reviews. 29; 851-875.
19. Gürtürk, K., Alan, M., Boynukara, B., Solmaz, H. (1994): Van ve Yöresinde Koyun ve Sıgır Brucellozisinin nsidensi Üzerinde Sero-Epidemiyolojik Arastırmalar. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi; 5: 121-125.
20. Hill, D., Dubey, J. P. (2002): Toxoplasma gondii: transmission, diagnosis and prevention. Clin Microbiol Infect. 8: 634-640.
21. Jittapalapong, S., Sangvaranond, A., Pinyopanuwat, N., Chimnoi, W., Khachaeram, W., Koizumi, S., Maruyama, S. (2005): Seroprevalence of Toxoplasma gondii infection in domestic goats in satun province, Thailand. Veterinary Parasitology. 127; 17-22.
22. Lhopital, S., J. Marty, P. Pardon, and P. Berche, (1993): Kinetics of antibody production against listeriolysin- O in sheep with listeriosis. J. Clin. Microbiol. 31, 1537-1540.
23. Low, J.C., Donachie, W. (1997): Reweiv of Listeria monocytogenes and Listeriosis. The Veterinary Journal, 153 (1): 9-29.
24. Low, J. C., and Donachie, W. (1991): Clinical and serum antibody responses of lambs to infection by Listeria monocytogenes. Res. Vet. Sci. 51, 189-192.
25. Low, J. C., R. C. Davies, and W. Donachie, (1992): Purification of listeriolysin-O and development of an immunoassay for diagnosis of listeric infections in sheep. J. Clin. Microbiol. 30, 2705-2708.
26. Masala, G., Porcu, R., Madau, L., Tanda, A., Ibba, B., Satta, G., Tola, S. (2003): Survey of ovine and caprine toxoplasmosis by IFAT and PCR assays in Sardinia, Italy. Veterinary Parasitology. 117; 15-21.
27. McLauchlin, J. (1996): The Relationship between Listeria and Listeriosis. Food Control. 7(4-5): 187-193.
28. Minas, A. (2006): Control and eradication of brucellosis in small ruminants. Small Ruminant Research. 62; 101-107.
29. Osebold, J., Aalund, O., Chrisp, C. E. (1965): Chemical and immunological composition of surface structures of Listeria monocytogenes. J. Bacteriol. 89: 84-86.
30. Portantı, O., Tıttarelli, M., Febo, D., Luciani, M., Mercante, M. T., Conte, A., and Lelli, R. (2006): Development and Validation of a competitive ELISA Kit for the serological diagnosis of ovine, caprine and Bovine Brucellosis. J. Vet.Med. B. 53; 494-498.
31. Rekha Bhanu, V., Malik, S. V. S., Chaudhari, S. P., Barbuddhe, S. B. (2006): Listeriolysin O-based diagnosis of Listeria monocytogenes infection in experimentally and naturally infected goats. Small Ruminant Research. 66; 70-75.
32. Shareef, J.M. (2006): A Review of Serological Investigations of Brucellosis among Farm Animals and Humans in Northern Provinces of Iraq (1974-2004). J. Vet.Med. B. 53; 38-40.
33. Skovgaard, N., and C. Morgen, (1988): Detection of Listeria spp. in faeces from animals, in feeds, and in raw foods of animal origin. Int. J. Food Microbiol. 6 ,229-242.
34. Taleski, V., Zerva, L., Kantardjiev, T., Cvetnic, Z., et.al. (2002): An overview of the epidemiology and epizootology of brucellosis in selacted countries of central and southeast Europe. Veterinary Parasitology. 90; 147-155.
35. Tütüncü, M., Akkan, H. A., Babür, C., Ayaz, E., Karaca, M. (2001): The seroprevalance of toxoplasma gondii in sheep detected by Sabin Feldman Dye Test in the region of Van, Turkey. Y.Y.U. Vet. Fak. Derg. 12 (1-2): 33-35.
36. Tütüncü, M., Ayaz, E., Yaman, M., Akkan, H. A. (2003): The seroprevalance of toxoplasma gondii in sheep, goats and cattle detected by indirect hemagglutination (IHA) test in the region of Van, Turkey. Indian Vet. J 80: 401-403.
37. Tütüncü, M., Solmaz, H., Akkan, H.A., Karaca, M., Agaoglu, Z. (2005): The Investigation of Listeria monocytogenes in cattle detected by ELISA test in the region of Van, Turkey, Indian Vet. J, 82, 926-928.
38. Ueno, H., Yokota, K., Arai, T., Muramatsu, Y., Taniyama, H., Iida, T., Morita, C. (1996): The prevalence of Listeria monocytogenes in the environment of dairy farms. Microbiol Immunol. 40(2):121-24.
39. Unnerstad H, Romell A, Ericsson H, Danielsson-Tham ML, Tham W. (2000): Listeria monocytogenes in faeces from clinically healthy dairy cows in Sweden. Acta Vet Scand. 41(2):167-71.