Afyonkarahisar'da tüketime sunulan çiğ süt ve peynirlerde E.coli 0157:H7 varlığının belirlenmesi

Bu çalışmada, Afyonkarahisar il merkezinde bulunan çeşitli bakkal ile marketlerden satın alınan 100 adet çiğ süt ve semt pazarlarından satın alınan 100 adet beyaz peynir örneğinde E. coli 0157:H7 varlığı ve yaygınlığı araştırılmıştır. E. coli O157:H7’nin izolasyon ve identifikasyonu’nda İmmuno Manyetik Separasyon (İMS) metodu ile Amerikan Gıda ve İlaç Dairesinin (Food and Drug Administiration/FDA) önerdiği metot kullanılmıştır. Analize alınan 100 adet çiğ süt örneğinden 3’ünde (%3) ve 100 adet peynir örneğinden 1’inde (%1) E. coli O157:H7 tespit edilmiştir. Sonuç olarak, çiğ süt ile beyaz peynirlerin E. coli O157:H7 ile bulaşmada kaynak olabileceği ve halk sağlığı açısından potansiyel bir tehdit oluşturabileceği kanaatine varılmıştır.

The prevalance of E.coli 0157:H7 in raw milks cheases sold in Afyonkarahisar province,Turkey

The purpose of this study was to identify the presence of E.coli 0157:H7 and its prevalence in raw milk and white cheese for which 100 units of raw milk were bought from the shops and markets of Afyonkarahisar provincial center and 100 white cheese samples were bought from neighborhood and villages markets. E. coli O157:H7 isolation and identification was carried out using the Immunomagnetic Separation (IMS) method and the method recommended by the American Food and Drug Administration (FDA). Out of the 100 raw milk samples, 3 (3%) samples and 1 (1%) of the cheese samples out of 100 were identified with having E. coli O157:H7. It is concluded that the presence of E.coli 0157:H7 in raw milk and white cheeses can be a source of contamination and is a potential public health hazard.

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  • 1. Abdul-Raouf U M, Ammar M S, Beuchat L R (1996): Isolation of Escherichia coli O157:H7 from some Egyptian foods, Int J Food Microbiol, 29: 423-426.
  • 2. Akkaya L, Alisarlı M, Atabay H, Kenar B (2005a): The prevalence of verocytotoxigenic E. coli O157:H7 in poultry meat sold in Afyon province. p123, No: 16, 1st. Int Food and Nutrition Congress, 15-18 June 2005, İstanbul.
  • 3. Akkaya L, Kenar B, Atabay H, Alisarlı M (2005b): The prevalence of verocytotoxigenic E. coli O157:H7 in minced meat and ground beef sold in Afyon province. P52, 1st. Int Food and Nutrition Congress, 15-18 June 2005, İstanbul.
  • 4. Akkaya L, Kenar B, Cetinkaya Z, Alisarlı M (2005c):The Prevalence of Verocytotoxigenic Escherichia coli 0157:H7 in Faeces from Cattle, in Afyon Province, Turkey (Unpublished Research Data)
  • 5. Aksu H, Arun Ö Ö, Aydın A, Ugur M (1999): Escherichia coli O157:H7’nin hayvansal kökenli çesitli gıda maddelerinde varlıgı, Pendik Vet Mikrobiyol Derg, 30(2): 77-81.
  • 6. Alisarlı M, Solmaz H, Akkaya L (2003): Süt ineklerinde meme bası derisinin ve çig sütün mikrobiyolojik kalitesi, Bildiri No: P42, SEYES Süt Endüstrisinde Yeni Egilimler Sempozyumu, 22-23 Mayıs 2003, İzmir.
  • 7. Armstrong G L, Hollingsworth J, Morris J G. (1996): Emerging food borne pathogens: Escherichia coli O157:H7 as a model of entry of a new pathogen into the food supply of the developed world, Epidemiol. Rew, 18: 29-51.
  • 8. Arnold K W Kapsar C W (1995): Starvation-and stationary phase-induced acid tolerance in Escherichia coli O157:H7. Applied and Environmental Microbiology, 61: 2037-2039.
  • 9. Aslantas Ö, Yıldız P (2002): Kars yöresinde hayvansal kaynaklı gıdalarda Escherichia coli O157:H7 izolasyonu, Vet Bil Derg, 18, 1-2, 107-111.
  • 10. Beser R E, Lett S M, Weber J T, Doyle M P, Baret T S, Wells J G. (1993): An outbreak of diarrhea and hemolytic uremic syndrome from Escherichia coli O157:H7 in freshpressed apple cider, J. Am. Med. Assoc, 269: 2217-220.
  • 11. Boyce T G, Swerdlow D L, Griffin P M (1995): Escherichia coli O157 and the hemolytic-uremic syndrome. New Engl. J. Med. 33: 364-368.
  • 12. Brewster D H, Brown M I, Robertson D, Houghton G L, Brimson J, Sharp J C M (1994): An outbreak of Escherichia coli O157 associated with a children's paddling pool, Epidem. Infect, 112: 441-447.
  • 13. Burnens A P, Frey A, Lior H, Nicolet J (1995): Prevalence and significance of verocytotoxin-producing Escherichia coli (VTEC) isolated from cattle in herds with and without calf diarrhea. Zentr. Veterin. (B), 42: 311-318.
  • 14. Carter A O, Borczyk A A, Carlson J A K, Harvey B, Hockin J C, Karmali M A, Kriskna C, Korn D A, Lior H (1987): A severe outbreak of Escherichia coli O157:H7- associated haemorrhagic colitiss in a nursing home. New Engl. J. Med. 317: 1496-1500.
  • 15. China B, Pirso V, Mainil J (1998): Prevalence and molecular typing of attaching and effacing Escherichia coli among calf populations in Belgium. Vet. Microbiol. 63: 249-259.
  • 16. Coia, J E (1998): Clinical, microbiological and epidemiological aspects of Escherichia coli O157 infection. FEMS Immunology and Medical Microbiology, 20: 1-9.
  • 17. Conedera G, Marangon S, Chapman P A, Zuin A, Caprioli A (1997): Atypical strains of verocytotoxinproducing Escherichia coli O157 in beef cattle at slaughter in Venato region, Italy. 44: 301-306.
  • 18. Coskun H (2000): Bazı Süt sletmelerinde Üretilen Beyaz ve Kasar Peynirlerinin Mikrobiyolojik ve Kimyasal Kalite Kriterleri Yönünden ncelenmesi, Süt Mikrobiyolojisi ve Katkı Maddeleri, Ed, Demirci M, Rebel Matbaacılık, istanbul.
  • 19. Çelik S, Özdemir C, Özdemir S, Sert S (1998): Diyarbakır Yöresinde Tüketime Sunulan Salamura Beyaz Peynir Örneklerinin Mikrobiyolojik, Fiziksel ve Kimyasal Özellikleri, Geleneksel Süt Ürünleri, Ed, Demirci M, MPM Yayınları No: 621, Ankara.
  • 20. D’ Aubert S, Cantoni C, Sarti A (1995): Detection of Escherichia coli O157:H7 in foods of animal origin, Arch Vet Ital, 46, 3, 67-72.
  • 21. Deschenes G, Casenave C, Grimont F, Desenclos J C, Benoit S, Collin M, Baron S, Mariani P, Grimont P A D, Nivet H (1996): Cluster of cases of haemolytic uremic syndrome due to unpasteurised cheese, Pediatr Nephrol, 10(2): 203-205.
  • 22. Desmarchelier P M, Grau E H (1997): Escherichia coli, p 231-236. In: A.D. Hocking, G., Arnold, I., Jenson, K., Newton, Shuterlan, P. (cd), Food borne microorganisms of public health significance. Australian Institute of Food Science and Technology Inc., Sydney, Australia.
  • 23. Dev V J, Mai M, Gould I (1991) : Waterborne outbreak of Escherichia coli O157, Lancet, 337: 1412.
  • 24. Doyle M P (1991): Escherichia coli O157:H7 and its significance in foods. Int J Food Microbiol., 12 (4):289-301..
  • 25. Duncan L, Mai V, Carter A, Carlson J A K, Borczyk A, Karmali M A (1987): Outbreak of gastrointestinal disease, Ontorio. Can. Dis. Weekly Rep., 13(2): 5-8.
  • 26. Erdogan A, Sert S (1988): Erzurum piyasasında satılan çig sütlerin antibiyotik ve mikrobiyolojik yönden incelenmesi, Dogu Anadolu Tarım Kongresi, 1677-1686.
  • 27. Ergüllü E (1982) Çig sütlerde koliform grubu bakteri florası üzerinde arastırmalar. Gıda, 7(6):263-266.
  • 28. Food and Drug Administration (2001): In: “Bacteriological Analytical Manual Online”, .www.fda.gov.
  • 29. Gönül S A (1997): Çig süt ve peynir örneklerinde Enterohemorajik E. coli’ye (O157:H7) rastlanma sıklıgı, Kükem Derg, 20(2): 69-73.
  • 30. Heescen W H (1998). “Çig Sütte Patojen Mikroorganizmalar”, Çevirenler, Ö Kınık, S Gönç ve S Akalın, Bölüm 7, 97-114, Ege Üniv Zir Fak Yay No:527, izmir.
  • 31. Heuvelink, A E, Van Den Biggelear F L, Zwartkruis- Nahuis J, Herbes R G, Huyben R, Nagelgerke N, Mechers W L, Monnens L A, Boer E (1988): Occurance of verocytotoxinproducing Escherichia coli O157 on Dutch dairy farms. J. Clin. Microbiol. 36: 3480-3487.
  • 32. Jones I G, Roworth M (1996): An outbreak of Escherichia coli O157 and campylobacteriosis with contamination of a drinking water supply, Pub. Health. 110: 277-282.
  • 33. Kıvanç M, Kunduhoglu B, Ayaz B (1992): Eskisehir’de tüketilen çig sütlerin bakteriyolojik kalitesinin halk saglıgı açısından incelenmesi, Gıda, 17(5):327-333.
  • 34. Kuntz T B Kuntz S T (1999): Enterohemorrhagic Escherichia coli infection, Prim. Care Uptade Ob/Gyn 6: 192-196.
  • 35. Martin M L, Shipman L D Wells G J (1986): Isolation of Escherichia coli O157:H7 from dairy cattle associated with to cases of hemolytic uremic syndrome, Lancett ii, 1043.
  • 36. McClure P (2000): The impact of E. coli O157 on the food industry. World J Microbiol Biotechnol, 16, 749-755.
  • 37. Mead P S, Griffin P M (1998): Escherichia coli O157:H7. Lancet, 352: 1207-1212.
  • 38. Mechie S C, Chapman P A, Siddons C A, (1998): A fifteen month study of Escherichia coli O157:H7 in a dairy herd. Epidem. Infect. 118: 17-25.
  • 39. Morgan D, Newman C P, Hutchinson, D N, Walker A M, Rowe B, Majid F. (1988): Verotoxin -producing Escherichia coli O157 infections associated with the consumption of yogurt. Epidem. and Infect. 111: 181-187.
  • 40. Özalp E, Kaymaz S ve Aksehirli E. (1978): Erzincan Tulum Peynirlerinde Enterotoksijenik Stafilokok’lar ve Salmonella’lar Yönünden Arastırma, A. Ü. Vet. Fak. Derg., 25(11): 56-61.
  • 41. Özdemir S, Çelik S, Özdemir C ve Sert (1998): Diyarbakır’ın Karacadag Yöresinde Mahalli Olarak Üretilen Örgü peynirlerinin Mikrobiyolojik ve Kimyasal Özellikleri, Geleneksel Süt Ürünleri, Ed, Demirci M, MPM Yayınları No: 621, Mert Matbaası, Ankara.
  • 42. Padhye N V, Doyle M P (1992): Escherichia coli O157:H7 epidemiology pathogenesis and methods for detection in food, J Food Prot, 55(7): 555-565.
  • 43. Patır B, Arslan A, Güven A (1995): Savak Salamura Beyaz Peynirlerinin Mikrobiyolojik Kalitesi, Fırat Üni. Vet. Bil. Derg., 11(1): 51-56.
  • 44 .Robinson R K, Batt C A, Patel P D (2000): Encyclopedia of Food Microbiology, Academic Press, London.
  • 45. Sanz M E, Vians M R, Parma A E (1998): Prevalence of bovine verotoxin-producing Escherichia coli in Argentina. J. Epidemiol. 14: 399-403.
  • 46. Sezgin E, Bektas S (1988): Trabzon’da satılan sokak sütlerinin bazı nitelikleri üzerine arastırmalar, Gıda, 13(6):181- 287
  • 47. Sharp J C M, Coia J E, Curnow J, Reilly W J (1994): Escherichia coli O157 infections in Scotland. J. Med. Microbiol. 40: 3-9.
  • 48. Sidjabat-Tambunan H, Bensink J C, (1997) Verocytotoxinproducing Escherichia coli from the faeces of sheep, calves and pigs. Aust. Vet. 75: 92-293.
  • 49. Upon P, Coia J E (1994): Outbreak of Escherichia coli O157 infections associated with pasteurized milk supply. Lancet, 334: 1015.
  • 50. Ünlütürk A (1998): Süt ve Süt Ürünlerinde Mikrobiyolojik Bozulmalar, Patojen Mikroorganizmalar Ve Muhafaza Yöntemleri, Gıda Mikrobiyolojisi, Ed, Ünlütürk A. ve Turantas F., 298-307, Mengi Tan Basım Evi, izmir.
  • 51. Watanabe H, Wada A, Inagaki Y (1996): Outbreaks of enterohaemorrhagic Escherichia coli O157 infection by two different genotype strains in Japan 1996, Lancet. 348: 831-832.
  • 52. Wells J G, Shipman L D, Greene K D, Sowers E G (1991): Isolation Escherichia coli serotype O157:H7 and other Shiga like toxin producing E. coli from dairy cattle, J Clin Microbiol, 29(5): 985-989.
  • 53. Whipp S C, Rasmussen M A, Cray W C (1994): Animals as a source of Escherichia coli pathogenic for human beings. J. Am. Vet. Med. Assoc., 204: 1168-1175.
  • 54. WHO. (1997): Prevention and control enterohaemorrhagic Escherichia coli (EHEC) infections: Report of a WHO consultation. Geneva.
  • 55.Yalçın H, Gökalp H, Özdemir Y (1991): Çig inek sütlerinden izole edilen koliform grubu ve S. aureus bakterilerinin tanımlanması. Gıda, 16(2): 107-110.
Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi-Cover
  • ISSN: 1017-8422
  • Yayın Aralığı: Yılda 3 Sayı
  • Başlangıç: 1990
  • Yayıncı: Yüzüncü Yıl Üniv. Veteriner Fak.