Elektrik Alanın, Rat Eritrosit ve Dokularındaki Antioksidan Enzim Süperoksid Dismutaz, Glutatyon Peroksidaz Aktiviteleri, Lipid Peroksidasyonu ve Glutatyon Seviyelerine Etkisi

Sunulan çalışmada doğru DC , alternatif akım AC ile trafo merkezinde yüksek voltaj altında TR sırasıyla, 0.74 kV/m, 0.81 kV/m ve 50 kV/m şiddetinde elektrik alan etkisine bırakılan rotların eritrosit ve dokularında, süperoksid dismutaz SOD , glutatyon peroksidaz GSH-Px aktiviteleri, lipid peroksidasyonu malondialdehid MDA ve glutatyon GSH düzeyleri araştırıldı. 44 genç erkek rat, 11 hayvandan oluşan 4 gruba ayrıldı. Birinci grup kontrol olarak alındı. Diğer üç grup 40 gün boyunca günde 24 saat süreyle farklı şiddetteki elektrik alan etkisine bırakıldı. Tüm ratlardan 20. ve 40. günlerde kan örnekleri alındı. 44. günde karaciğer ve beyin dokuları kesilerek çıkartıldı. MDA, GSH, SOD ve GSH-Px kan ve dokulardaki düzeyleri, spektrofotometrik olarak ölçüldü. Kan MDA düzeyi, DC ve AC gruplarında kontrol grubuna göre artarken, kan glutatyon düzeyi bütün gruplarda önemli oranda azaldı p

Anahtar Kelimeler:

Elektrik alan, Antioksidanlar, Malondialdehid, Eritrosit, Doku

Effect of Electric Field on Activities of Antioxidaııt Enzymes SOD, GSH-Px , Lipid Peroxidation MDA and Glutathioııe Levels in Rat Erythrocytes and Tissues

Effect of Electric Field on Activities of Antioxidaııt Enzymes SOD, GSH-Px , Lipid Peroxidation MDA and Glutathioııe Levels in Rat Erythrocytes and Tissues The main aim of this study was to investigate activity of SOD, GSH-Px as w e ll as MDA and GSH levels in the rat erythrocytes and tissues exposed to ele ctric field of dire ct current DC , alternating cıırrent AC and Van Transformer Çenter TR with intensity of 0.74 kV/m, 0.81 kV/m and 50 kV/m respectively. Forty-four adult male rats were divided into four groups of 11 animals. The first group was used as the control KN . The other groups were exposed to three different electric fields described abovefor 24 hours till 40th day. Blood samples were collected on 20th and 40rh days. Liver and brain tissues were excised on the 44th day. MDA, GSH, SOD and GSH-Px w e re determined on both tissues and blood samples by means of spectrophotometer. In blood, MDA levels in DC and AC groups were found to be signifıcantly increased comparing to other groups p

Keywords:

Electric field, Erythrocytes, Malondialdehyde, Antioxidcınt, Tissue.,

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