Sağıtım Dozlarında Uygulanan Bazı Sulfonamidlerin Alabalıkların Oncorhynchus mykiss Yenilebilir Dokularında Kalıntı Düzeyleri ile Vücuttan Atılma Sürelerinin Belirlenmesi

Bu çalışmada, Gökkuşağı alabalıklarında tedavi amacıyla kullanılan sulfadiazin, sulfamerazin, sulfametazin, sulfametoksazol ve sulfadimetoksinin dokulardaki kalıntı düzeylerinin ne kadar zamanda tolerans limitlerinin altına düştüğü, ilaç uygulamasından ne kadar süre sonra tüketime sunulması gerektiği ve sillfonamid grubu ilaçlar uygulandığı zaman dokularda hangi düzeylerde bulunduğu Yüksek Basınçlı Sıvı Kromatografısi HPLC ile araştırılmıştır. Gökkuşağı alabalıkları 3 ay süreyle su ortamına ve yeme alıştırıldıktan sonra canlı ağırlıkları tartılarak, grup ortalamaları birbirine yakın olacak şekilde 180 adet balık, her grupta 36 adet olacak şekilde 5 gruba ayrıldı. Grubun herbirine bir sulfonamid türevi 200 mg/kg/gün dozunda 14 gün süreyle uygulandı. Balıklar, en son ilaç uygulamasından sonra L, 3., 5., 7., 9., 11., 13., 15., 17., 19. ve 21 günlerde ilçerli gruplar halinde alınarak kas dokuları blenderde parçalanarak homojen hale getirildi. Bu örneklerin ekstraksiyon işleminden sonra, sulfadiazin, sulfamerazin, sulfametazin, sulfametoksazol ve sulfadimetoksinin kas dokusundaki dağılımları Yüksek Basınçlı Sıvı Kromatografısi Metoduyla tesbit edildi. Sulfadiazin 0.097 ±0.01 ppm , sulfamerazin 0.1 ±0.02 ppm , sulfametazin 0.07 ±0.02 ppm ve sulfametoksazol 0.081 ±0.043 ppm 11. günde, sulfadimetoksinin ise 0.095 ±0.02 ppm 7. günde tolerans limitinin altına indiği saptandı. Gökkuşağı alabalığı kas dokusunda sulfadiazin 17. günde 0.018 ±0.003 ppm, sulfamerazin 19. günde 0.01 ±0.002 ppm, sulfametazin 17. günde 0.01 ±0.001 ppm, sulfametoksazol 15. günde 0.004 ±0.001 ve sulfadimetoksin ise 11. günde 0.003 ±0.0005 ppm düzeyinde belirlendi. Gökkuşağı alabalıklarının sulfadiazin, sulfamerazin, sulfametazin ve sulfametoksazol için 11. günden, sulfadimetoksin için ise 7. günden sonra tüketime sunulabileceği sonucuna varıldı

Anahtar Kelimeler:

Sıvı kromatografısi, Sulfonamidler, İlaç kalıntıları, Gökkuşağı alabalığı

The determiııation of residues in edible tissues and withdrawal times of some sulfanamides, administered with therepeutic doses to Rainbow trout

In tlıis study, sulfadiazine, sulfamerazine, sulfamethazine, sulfamethoxazole and sulfadimethoxine were applied to the rainboyv troııts. II o w long it takes to lower below tolerance limits for the resııdue levels accumulated in tissues, consumption time after medication, and accurance levels of sulfonamide group in tissues were researched by using HPLC. The experimeııtal rainbow trouts were adapted for the feed and water conditious for a period of three months. After their body were weighed and total 180 rainbow trouts were devided into five groups. Each group coııtained 36 fishes. Each group was treated with. a sulfonamide derivative 200 mg/kg/day for 14 days. After the latest applicatioıı of mediciııes, livefish samples were taken on 1, 3, 5, 7, 9, 11, 13, 15, 17, 19 and 21 days, and their muscle tissue were homogenized with blender. After extraction process of the samples, distributions of sulfadiazine, sulfamerazine, sulfamethazine, sulfamethoxaz.ole and sulfadimethoxine in muscle tissue were determined by HPLC. it was found that tolerance limits of sulfadiazine 0.097 ±0.01 ppm , sulfamerazine 0.100 ±0.02 ppm , sulfamethazine 0.070 ±0.02 ppm and sulfamethoxazole 0.081 ±0.04 ppm lowered on 1 lth day. However, tolerance limit of sulfadimethoxine 0.095 ±0.02 ppm lowered on 7th day. Sulfadiazine, sulfamerazine, sulfamethazine, sulfamethoxazole and sulfadimethoxine were determined as 0.018 ±0.003 ppm on 17th day, 0.010 ±0.02 ppm on 19th day, 0.010 ±0.001 ppm on 17th day, 0.004 ±0.001 ppm on 15th day and as 0.003 ±0.0005 ppm on 1 lth day in the tissue ofrainbow trouts, respectively. İt was also determined that the raiııbow trouts would be cıble to be consumed after 1 lth day in terms of sulfadiazine, sulfamerazine, sulfamethazine and sulfamethoxazole. Nevertheles, it was after 7th day for sulfadimethoxine.

Keywords:

Liquid ehromatography, Sulfanamides, Drııg residues, Rainbow trout,

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