Lektin verilen normal ve tümörlü farelerde serum, karaciğer ve böbrek AST, ALT, GGT, ALP, CK aktiviteleri

Bu çalışmada, antikarsinojen bir madde olarak da kullanılan Viscum album Aglutinin (VAA)'nın doz aşımı uygulamalarının serum, karaciğer ve böbrek aspartat transaminaz (AST), alanin transaminaz (ALT), gama-glutamiltransferaz (GGT), alkalen fosfataz (ALP) ve kreatin kinaz (CK) enzim aktiviteleri üzerine olan etkisinin ortaya çıkarılması amaçlanmıştır. Çalışmada deneme materyali olarak, 80 adet BALB/c erkek fare kullanılmış ve dört eşit gruba ayrılmıştır. Donör bir. fareden 0,1ml (l,2xl06) Erlich ascites hücresi alınmış ve bu hücreler kanserli kontrol ve lektin deneme grubuna periton içi (i.p.) olarak uygulanmıştır. Uygulama günü 0. gün olarak kabul edilmiştir. Aynı gün, normal kontrol ve lektin kontrol gruplarına 0,1 mi. serum fizyolojik i.p. olarak verilmiştir. Lektin kontrol ve lektin deneme grubuna, 1. ve 2. günlerde lml/fare (lOOng/fare) VAA verilirken, normal kontrol ve kanserli kontrol gruplarına serum fizyolojik verilmiştir. Tüm gruplardan, kan örnekleri 2., 7. ve 15. günlerde göz venasından kapillar tüpler aracılığı ile serum tüplerine alınmıştır. Tüm gruplardan, 2. günde 5 hayvan, 7. günde 5 hayvan, 15. günde ise 10 hayvan otopsiye gönderilmiştir. Otopside, farelerden karaciğer ve böbrek örnekleri alınmıştır. Serum, karaciğer ve böbrek üst sıvısında AST, ALT, GGT, ALP, CK enzim aktiviteleri ölçülmüştür. Biyokimyasal analizlerin sonucunda, kontrol grublarına göre lektin deneme grubunda serum AST, ALT, GGT, ALP ve CK aktiviteleri yükselmiştir. Tüm kontrol gruplarına göre lektin deneme grubunda karaciğer AST, ALT, GGT ve ALP spesifik aktiviteleri yükselirken, CK spesifik aktivitesi değişmemiştir. Kontrol gruplarına göre lektin deneme grubunda böbrek AST, ALT, GGT ve ALP spesifik aktiviteleri yükselirken, kanserli kontrol grubuna göre lektin deneme grubundaki enzim spesifik aktivitelerinde anlamlı değişimler bulunamamıştır. Enzim aktivitelerindeki bu artışların VAA'nın toksik etkisinden kaynaklanabileceği şeklinde yorumlanmıştır.

Anahtar Kelimeler:

Viscum album, böbrekler, hayvan dokuları, alkalin fosfotaz, antineoplastik ajanlar, lektin, karaciğer, gama-glutamiltransferaz, enzim aktivitesi, kreatin kinaz, alanin aminotransferaz, aspartat aminotransferaz, neoplazma, fareler

AST, ALT, GGT, ALP, CK activities in serum, liver and kidney tissues in lectin applied normal mice and mice with tumour patient

The aim of the study was observed the effect of over dose application of Viscum album Agglutinine (VAA) which is used as a antikarsinogenic agent on the activities of aspartate aminotransferase (AST), alkaline aminotransferase (ALT), gamma-glutamyltransferase (GGT), alkaline phosphatase (ALP) and creatine kinase (CK) enzymes in serum, liver and kidney. As experimental material, 80 BALB/c male mice were used and divided into four equal groups. 0,1ml (l,2xl06) Erlich ascites cells were taken from a donor mouse and those cells were given cancer control and lectin trial groups by intraperitoneal (i.p.). Application day was accepted as day 0. In the same day, 0,1 ml serum physiologic was given normal control and lectin control groups by i.p. While lml/mause (lOOng/mause) VAA was giving lectin control and lectin trial groups by i.p. on days 1 and 2. 0,1 ml/mouse serum physiologic was given normal control and lectin control groups. From whole groups, the blood samples were taken from ocular vena via capillary tubes into serum tubes on days 2, 7 and 15. 5 animals on days 2, 5 animals on days 7, 10 animals on day 15 were sent to autopsy. In the autopsy, samples of liver and kidney tissues were taken frommice. AST, ALT, GGT, ALP, CK enzymes activities were measured in serum and supernatant of liver and kidney. At the result of biochemical analyses, serum AST, ALT, GGT, ALP, CK activities increased in lectin trial group in comparison with whole control groups. While liver AST, ALT, GGT, ALP specific activities increased in lectin trial group in compare with whole control groups, CK specific activity did not change. While kidney AST, ALT, GGT, ALP specific activities increase in lectin trial group in comparison with normal control and lectin control groups, In the enzymes specific activities did not found significant chances in lectin trial group in compare with cancer control group. It was commended that the increase of enzymes activities can be from toxic effect of VAA

Keywords:

Viscum album, kidneys, animal tissues, alkaline phosphatase, antineoplastic agents, lectins, liver, gamma-glutamyltransferase, enzyme activity, creatine kinase, alanine aminotransferase, aspartate aminotransferase, neoplasms, mice,

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