Influence of different proteinaceous diets on consumption, brood rearing,and honey bee quality parameters under isolation conditions
Influence of different proteinaceous diets on consumption, brood rearing,and honey bee quality parameters under isolation conditions
This study was carried out to evaluate the efficacy of 5 proteinaceous diets on honey bees under isolation conditions. The diets were soybean meal, mesquite pod powder, date paste, Feedbee®, and corn gluten, along with control colonies (bees fed naturally on pollens). These 5 diets were supplied to honey bee colonies under isolation conditions, except for the control group. Food consumption amount, brood rearing activity, and some physiological parameters of the honey bees were measured. The best consumption amount was for date paste, followed by Feedbee, mesquite, and corn gluten, respectively. Concerning brood rearing activity, the highest mean of sealed brood cells was 174.7 cells/colony in colonies fed on Feedbee, while no brood was reared in colonies fed on soybean meal. The fresh weight, dry matter, and protein content of fully grown larvae and newly emerged workers were higher in colonies fed on bee bread than the colonies fed on the artificial diets. Total soluble solids %, total hemolymph count, and hemolymph protein % were varied among tested diets. Feedbee was able to enhance brood rearing, but Feedbee is expensive and not available in the local markets of Saudi Arabia. Hence, beekeepers are advised to use mesquite pod flour as a pollen substitute in dearth periods
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- 1. Brodschneider R, Crailsheim K. Nutrition and health in honey bees. Apidologie 2010; 41: 278-294.
- 2. Herbert EW Jr. Honey bee nutrition. In: Graham JM, editor. The Hive and the Honey Bee. 1st ed. Hamilton, IL, USA: Dadant and Sons; 1992. pp. 197-233.
- 3. Bounias M, Morgan MRJ. Effect of sucrose feeding on the induction of honey bee haemolymph alpha-glucosidase. J Apic Res 1990; 29: 181-186.
- 4. Herbert EW, Bickley WG, Shimanuki H. The brood rearing capacity of caged honey bees fed dandelion and mixed pollen diets. J Econ Entomol 1970; 63: 215-218.
- 5. Herbert EW, Shimanuki H, Caron D. Optimum protein levels required by honey bees (Hymenoptera, Apidae) to initiate and maintain brood rearing. Apidologie 1977; 8: 141-146.
- 6. Winston ML, Chalmers WT, Lee PC. Effects of two pollen substitutes on brood mortality and length of adult life in the honey bee. J Apic Res 1983; 22: 49-52.
- 7. Omar MOM. Influence of supplementary honeybee feeding with pollen and yeast paraffin to development haemolymph composition and enzymatic activity of Apis mellifera L. PhD. Institute of Agronomy, Bucharest, Romania, 1984.
- 8. Kleinschmidt GJ, Kondos AC. The influence of crude protein levels on colony performance. Aust Bee J 1977; 79: 357-361.
- 9. Mostafa AM. Influence of some supplementary feeding on physiological characters and productivity of honey bees. PhD, Assiut University, Assiut, Egypt, 2000.
- 10. Hrassnigg N, Crailsheim K. Differences in drone and worker physiology in honey bees (Apis mellifera L.). Apidologie 2005; 36: 255-277.
- 11. Glinski Z, Klimont S. Effect of Varroa jacobsoni invasion on the cells in the haemolymph of worker bees Apis mellifera L. Med Weter 1987; 43: 546-549.
- 12. Rogala R, Szymas B. Nutritional value for bees of pollen substitute enriched with synthetic amino acids. Part II. Biological methods. J Apic Sci 2004; 48: 29-36.
- 13. Cremonez T, DeJong D, Bittondi M. Quantification of hemolymph proteins as a fast method for testing protein diets for honey bees (Hymenoptera: Apidae). J Econ Entomol 1998; 91: 1284-1289.
- 14. Kirk PL. Kjeldahl method for total nitrogen. Anal Chem 1950; 22: 354-358.
- 15. Oduol PA, Felker P, Mckinley CR, Meier CE. Variation among selected Prosopis families for pod sugar and pod protein contents. Forest Ecol Manag 1986; 16: 423-431.
- 16. Saffari AM, Kevan PG, Atkinson JK. Feeding colonies with a nutritious pollen supplement is beneficial. Bee Culture 2006; 134: 30-31.
- 17. Hanna HM, Azab SG. Effects of larval population density on weights and components of Spodoptera exigua. Bull Soc Ent 1973; 57: 55-65.
- 18. Rabie AL, Wells JD, Dent LK. The nitrogen content of pollen protein. J Apic Res 1983; 22: 119-123.
- 19. Predetshensky VE, Parovska VM, Margolina LT. Microtechnique methods. Goso Uzd Medgez Moskva 1950; 125: 115-121 (in Russian with an English abstract).
- 20. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193: 265-275.
- 21. Duncan DB. Multiple range and multiple tests. Biometrics 1955; 11: 1-42.
- 22. Imdorf A, Kilchenmann V, Maquelin C. What is the effect of spring pollen feeding on the development of colonies? Liebefield Apiculture 1988; 85: 67-76 (in German with an English abstract).
- 23. Konopacka Z. Quality of honeybee families, strong and weak. Pszczeln Zesz Nauk 1974; 18: 161-173 (in Russian with an English abstract).
- 24. Konopacka Z, Muszynska J. Changing in the honeybee worker condition during growing season. Pszczeln Zesz Nauk 1981; 25: 3-14 (in Russian with an English abstract).
- 25. Dustmann JH, von der Ohe W. Influence of cold snap in the spring development of honeybee colonies (Apis mellifera L.). Apidologie 1988; 19: 245-254.
- 26. Kunert K, Crailsheim K. Seasonal changes in carbohydrate, lipid and protein content in emerging worker honey bees and their mortality. J Apic Res 1988; 27: 13-21.
- 27. Wille H, Imdorf A. The nitrogen supply of honeybee colonies. Allg Dtsch Imkerztg 1983; 17: 37-50 (in German with an English abstract).
- 28. Szymas B, Jedruszuk A. The influence of different diets on haemocytes of adult worker honey bees, Apis mellifera L.Apidologie 2003; 34: 97-102.
- 29. De Jong D, Da Silva EJ, Kevan PG, Atkinson JL. Pollen substitutes increase honey bee haemolymph protein levels as much as or more than does pollen. J Apic Res 2009; 48: 34-40.
- 30. Gregory P. Protein diets and their effects on worker weight, longevity, consumption and haemolymph protein levels of Apis mellifera L. In: Proceedings of the 19th American Bee ResearchConference. Baton Rouge, LA, USA: ABRC; 2006. pp. 9-10.