Research of the effects of autologous cancellous bone graft and hyaluronic acidon the healing of bone defects experimentally induced in rabbits
Research of the effects of autologous cancellous bone graft and hyaluronic acidon the healing of bone defects experimentally induced in rabbits
The present study investigated the effect of autograft and hyaluronic acid (HA) in terms of clinical, radiological, and histopathological aspects on the healing of experimentally induced bone defects. The animals (n = 42) were assorted into 3 groups. After general anesthesia, a 3-mm standard defect was formed from the tibia of the experimental animals. The defects in Group I (n = 14) were left blank. Cancellous grafts in the proximal tibia were collected using a small curette to fill the defects in Group II (n = 14). The defects in Group III (n = 14) were filled by mixing with HA and cancellous grafts taken from the same region of the tibia. To determine the course of healing in the 3 groups in terms of clinical, radiological, and histopathological aspects, 7 rabbits from each group were chosen randomly on days 35 and 70 postsurgery and euthanized under anesthesia. Consequently, higher scores in terms of bone healing criteria were obtained in Group II, in which the graft was applied, compared with Group I. In Group III, in which the graft material was combined with HA, more healing was achieved compared with both Group I and Group II.
___
- 1. Gronowicz G, Hurley MM, Kuhn LT. Optimizing BMP-2-induced bone repair with FGF-2. J Am Acad Orthop Surg 2014; 22: 1-3.
- 2. Lissenberg-Thunnissen SN, De Gorter DJ, Sier CF, Schipper IB. Use and efficacy of bone morphogenetic proteins in fracture healing. Int Orthop 2011; 35: 1271-1280.
- 3. Kawaguchi H, Oka H, Jingushi S Izumi T, Fukunaga M, Sato K, Matsushita T, Nakamura K. TESK Group: a local application of recombinant human fibroblast growth factor 2 for tibial shaft fractures: a randomized, placebo-controlled trial. J Bone Miner Res 2010; 25: 2735-2743.
- 4. King SR, Hickerson WL, Proctor KG. Beneficial actions of exogenous hyaluronic acid on wound healing. Surgery 1991; 109: 76-84.
- 5. Pilloni A, Bernard GW. The effect of hyaluronan on mouse intramembranous osteogenesis in vitro. Cell Tissue Res 1998; 294: 323-333.
- 6. Price RD, Berry MG, Navsaria HA. Hyaluronic acid: the scientific and clinical evidence. J Plastic Reconstr Aesth Surg 2007; 60: 1110-1119.
- 7. Tammi, R, Ripellino, JA, Margolis, RU, Tammi, M. Localization of epidermal hyaluronic acid using the hyaloronate binding region of cartilage proteoglycan as a specific probe. J Invest Dematol 1988; 90: 412-414.
- 8. Kadiyala S, Young RG, Thiede MA, Bruder SP. Culture expanded canine mesenchymal stem cells possess osteochondrogenic potential in vivo and in vitro. Cell Transplant 1997; 6: 125-134.
- 9. Sagliyan A, Gunay C, Han MC, Sakın F, Hayat A. An experimental study on the efficacy of sodium hyaluronate in prevention of postoperative intraperitoneal adhesions. J Anim Vet Adv 2009; 8: 664-668.
- 10. Sagliyan A, Karabulut E, Unsaldı E, Yaman I. Evaluation of the activity of intraarticular hyaluronic acid in the repair of experimentally induced osteochondral defects of the stifle joint in dogs. Vet Med-Czech 2009; 54: 33-40.
- 11. Sasaki T, Watanabe C. Stimulation of osteoinduction in bone wound healing by high-molecular hyaluronic acid. Bone 1995; 16: 9-15.
- 12. Laurent TC. Fraser JRE. Hyaluronan. FASEB J 1992; 6: 2397-2404.
- 13. Laurent TC, Laurent UB, Fraser JRE. The structure and function of hyaluronan: an overview. Imniunol Cell Biol 1996; 74: l-7.
- 14. Dimar JR, AnteWA, Zhang YP, Glassman SD. The effects of nonsteroidal anti-inflammatory drugs on posterior spinal fusions in the rat. Spine 1996; 21: 1870-1876.
- 15. Goldberg VM, Powell A, Shaffer JW, Zika J, Bos GD, Heiple KG. Bone grafting: role of histocompatibility in transplantation. J Orthop Res 1985; 3: 389-404.
- 16. Lane JM, Sandhu HS. Current approaches to experimental bone grafting. Orthop Clin North Am 1987; 18: 213-225.
- 17. Emery SE, Brazinski MS, Koka A, Bensusan JS, Stevenson S. The biological and biomechanical effects of irradiation on anterior spinal bone grafts in a canine model. J Bone Joint Surg Am 1994; 76: 540-548.
- 18. Radomsky ML, Aufdemorte TB, Swain LD, Fox WC, Spiro RC, Poser JW. Novel formulation of fibroblast growth factor-2 in a hyaluronan gel accelerates fracture healing in nonhuman primates. J Orthop Res 1999; 17: 607-614.
- 19. Huo MH, Troiano NW. The influence of ibuprofen on fracture repair: biomechanical, biochemical, histologic, and histomorphometric parameters in rats. J Orthop Res 1991; 9: 383-390.
- 20. Keller J, BUnger C, Andreassen TT, Bak B, Lucht U. Bone repair inhibited by indomethacin. Effects on bone metabolism and strength of rabbit osteotomies. Acta Orthop Scand 1987; 58: 379-383.
- 21. Luangphakdy V, Walker E, Shinohara K, Pan H, Hefferan T, Bauer TW, Stockdale L, Saini S, Dadsetan M, Runge MB et al. Evaluation of osteoconductive scaffolds in the canine femoral multi-defect model. Tissue Eng Part A 2013; 19: 634-648.
- 22. Oryan A, Alidadi S, Moshiri A. Current concerns regarding healing of bone defects. Hard Tissue 2013; 2: 13-24.
- 23. Bigham AS, Shadkhast M, Bigham Sadegh A, ShafIei Z, Lakzian A, Khalegi MR. Evaluation of osteoinduction properties of the demineralized bovine foetal growth plate powder as a new xenogenic biomaterial in rat. Res Vet Sci 2011; 91: 306-310.
- 24. Bigham AS, Shadkhast M, Khalegi MR. Demineralized calf foetal growth plate effects on experimental bone healing in rabbit model. Vet Arhiv 2013; 83: 525-536.
- 25. Friedlaender GE. Immune responses to osteochondral 5. allografts. Current knowledge and future directions. Clin Orthop Relat Res 1983; 174: 58-68.
- 26. Nandi SK, Roy S, Mukherjee P, Kundu B, De DK, Basu D. Orthopaedic applications of bone graft & graft substitutes: a review. Indian J Med Res 2010; 132: 15-30.
- 27. Solchaga LA, Dennis JE, Goldberg VM, Caplan AI. Hyaluronic acid-based polymers as cell carriers for tissue-engineered repair of bone and cartilage. J Orthop Res 1999; 17: 205-213.
- 28. Oakes DA, Lee CC, Lieberman JR. An evaluation of human demineralized bone matrices in a rat femoral defect model. Clin Orthop 2003; 413: 101-109.
- 29. Hunt DR, Jovanovic SA, Wikesjö UM, Wozney JM, Bernard GW. Hyaluronan supports recombinant human bone morphogenetic protein-2 induced bone reconstruction of advanced alveolar ridge defects in dogs. A pilot study. J periodontal 2001; 72: 651-658.
- 30. Aslan M, Simsek G, Dayi E. The effect of hyaluronic acid-supplemented bone graft in bone healing: experimental study in rabbits. J Biomater Appl 2006; 20: 209-219.