Comparative efficacy of antioxidant retinol, melatonin, and zinc during in vitro maturation of bovine oocytes under induced heat stress
Comparative efficacy of antioxidant retinol, melatonin, and zinc during in vitro maturation of bovine oocytes under induced heat stress
Both A and B grade cumulus-oocyte complexes (COCs) aspirated from cattle ovaries at slaughter were matured in vitro under normal (38.5 °C) and elevated temperatures (41 °C). Maturation competence based on cumulus expansion, COC diameter, and nuclear maturation were compared with and without antioxidant supplementation incorporating 7.5 μM retinol, 1 nM melatonin, and 1.5 μg/mL zinc chloride in an oocyte maturation medium. Heat stress significantly reduced cumulus expansion by approximately 20%, while only retinol could bring a significant (P ≤ 0.05) increase. Heat stress also decreased expansion of A (approximately 50%) and B (approximately 40%) grade COC diameter. All antioxidants significantly increased COC diameter at 38.5 °C in grade A COCs, but only retinol could significantly increase grade B COCs. At 41 °C, only retinol in grade A COCs significantly enhanced diameter. Elevated temperature also decreased the metaphase II stage of nuclear maturation by approximately 75%, and no antioxidant was protective, except retinol, which was only marginally so. Retinol (7.5 μM) was further supplemented in maturation and culture medium for in vitro embryonic development at 38.5 °C and demonstrated significantly higher (P ≤ 0.01) maturation, fertilization, and a 2 4 cell cleavage rate. Retinol supplementation not only showed better maturation results, but also was a better antioxidant in overcoming the deleterious effects of elevated temperature.
___
- 1. Torres-Junior JRD, Pires MDA, De Sa WF, Feffeira AD, Viana JHM, Camargo LSA, Ramos AA, Folhadella IM, Polisseni J, de Freitas C et al. Effect of maternal heat stress on follicular growth and oocyte competence in Bos indicus cattle. Theriogenology 2008; 69: 155-166.
- 2. Alves MF, Gonçalves RF, Pavão DL, Palazzi EG, Souza F, Ribeiro de Queiróz RK, DAngelo M, Antônio de Achilles M. Effect of heat stress on the maturation, fertilization and development rates of in vitro produced bovine embryos. Open J Anim Sci 2013; 3: 174-178.
- 3. Ikeda M, Kodama H, Fukuda J, Shimizu Y, Murata M, Kumagai J, Tanaka T. Role of radical oxygen species in rat testicular germ cell apoptosis induced by heat stress. Biol Reprod 1999; 61: 393-399.
- 4. Ali AA, Bilodeau JF, Sirard MA. Antioxidant requirements for bovine oocyte varies during in vitro maturation, fertilization and development. Theriogenology 2003; 59: 939-949.
- 5. Karaşahin T, Arıkan Ş. The effect of oleic and linoleic acids on in vitro bovine embryonic development and embryo quality. Turk J Vet Anim Sci 2015; 39: 154-159.
- 6. Hosseini SM, Forouzanfar M, Hajian M, Asgari V, Abedi P, Hosseini L, Ostadhosseini S, Moulavi F, Langrroodi MS, Sadeghi H et al. Antioxidant supplementation of culture medium during embryo development and/or after vitrification-warming; which is the most important? J Assist Reprod Genet 2009; 26: 355-364.
- 7. Livingston T, Eberhardt D, Edwards JL, Godkin J. Retinol improves bovine embryonic development in vitro. Reprod Biol Endocrinol 2004; 2: 83-88.
- 8. Tan DX, Manchester LC, Terron MP, Flores LJ, Reiter RJ. One molecule, many derivates: a never-ending interaction of melatonin with reactive oxygen and nitrogen species? J Pineal Res 2007; 42: 28-42.
- 9. Jeon Y, Yoon JD, Cai L, Hwang SU, Kim E, Zheng Z, Lee E, Kim DY, Hyun SH. Supplementation of zinc on oocyte in vitro maturation improves preimplatation embryonic development in pigs. Theriogenology 2014; 82: 866-874.
- 10. Dutta DJ, Dev H, Raj H. In vitro blastocyst development of post-thaw vitrified bovine oocytes. Vet World 2013; 6: 730-733.
- 11. Hafez ESE, Hafez B. Reproduction in Farm Animals. 7th ed. New York, NY, USA: Wiley; 2000.
- 12. Roth Z, Hansen PJ. Involvement of apoptosis in disruption of developmental competence of bovine oocytes by heat shock during maturation. Biol Reprod 2004; 71: 1898-1906.
- 13. Jang HY, Kong HS, Choi KD, Jeon GJ, Yang BK, Lee CK, Lee HK. Effects of melatonin on gene expression of IVM/IVF porcine embryos. Asian-Aust J Anim Sci 2005; 18: 17-21.
- 14. Farahavar A, Shahne AA, Kohram H, Vahedi V. Effect of melatonin on in vitro maturation of bovine oocytes. Afr J Biotechnol 2010; 9: 2579-2583.
- 15. Picco SJ, Anchordoquy JM, de Matos DG, Anchordoquy JP, Seoane A, Mattioli GA, Errecalde AL, Furnus CC. Effect of increasing zinc sulphate concentration during in vitro maturation of bovine oocytes. Theriogenology 2010; 74: 1141-1148.
- 16. Bracket BG, Oliphant G. Capacitation of rabbit spermatozoa in vitro. Biol Reprod 1975; 12: 260-274.
- 17. Cajuday LA, Herrera AA, Duran DH. Effect of retinoic acid on the development of water buffalo embryos in vitro. Philipp J Vet Anim Sci 2012; 38: 107-116.
- 18. Vahedi V, Zeinoaldini S, Kohram H, Farahavar A. Retinoic acid effects on nuclear maturation of bovine oocytes in vitro. Afr J Biotechnol 2009; 8: 3974-3978.
- 19. Bahadori MH, Ghasemian F, Ramezani M, Asgari Z. Melatonin effect during different maturation stages of oocyte and subsequent embryo development in mice. Iran J Reprod Med 2013; 11: 11-18.
- 20. Jeon Y, Yoon JD, Cai L, Hwang SU, Kim E, Lee S, Hyun SH. The effect of zinc on porcine in vitro maturation and subsequent embryonic development after in vitro fertilization. Reprod Fert Dev 2013; 26: 191-191.
- 21. Maya-Soriano MJ, Taberner E, López-Béjar M. Retinol improves in vitro oocyte nuclear maturation under heat stress in heifers. Zygote 2013; 21: 377-384.
- 22. Mohan M, Malayer JR, Geisert RD, Morgan GL. Expression of retinol-binding protein messenger RNA and retinoic acid receptors in preattachment bovine embryos. Mol Reprod Dev 2001; 60: 289-296.
- 23. Mohan M, Malayer JR, Geisert RD, Morgan, GL. Expression patterns of retinoid X receptors, retinaldehyde dehydrogenase, and peroxisome proliferator activated receptor gamma in bovine preattachment embryos. Biol Reprod 2002; 66: 692-700.
- 24. Old RW, Smith DP, Mason CS, Marklew S, Jones EA. Effects of retinoic acid on Xenopus embryos. Biochem Soc Symposium 1995; 62: 157-175.
- 25. Gómez E, Royo LJ, Duque P, Carneiro G, Hidalgo C, Goyache F, Lorenzo PL, Alvarez I, Facal N, Diez C. 9-cis-Retinoic acid during in vitro maturation improves development of the bovine oocyte and increases midkine but not IGF-I expression in cumulus-granulosa cells. Mol Reprod Develop 2003; 66: 247-255.
- 26. Takada L, Martins Junior A, Mingoti GZ, Balieiro JCC, Cipolla-Neto J, Coelho LA. Effect of melatonin on DNA damage of bovine cumulus cells during in vitro maturation (IVM) and on in vitro embryo development. Res Vet Sci 2012; 92: 124-127.
- 27. Adriaens I, Jacquet P, Cortvrindt R, Janssen K, Smitz J. Melatonin has dose-dependent effects on folliculogenesis, oocyte maturation capacity and steroidogenesis. Toxicology 2006; 228: 333-343.
- 28. Chattoraj A, Bhattacharyya S, Basu D, Bhattacharya S, Bhattacharya S, Maitra SK. Melatonin accelerates maturation inducing hormone (MIH): induced oocyte maturation in carps. Gen Comp Endocrinol 2005; 140: 145-155.
- 29. Cebrian-Serrano A, Salvador I, Raga E, Dinnyes A, Silvestre MA. Beneficial effect of melatonin on blastocyst in vitro production from heat-stressed bovine oocytes. Reprod Domest Anim 2013. 48: 738-746.
- 30. Komar A. Parthenogenetic development of mouse eggs activated by heat-shock. J Reprod Fertil 1973; 35: 433-443.