Gulbeena SALEEM, Rukhshanda RAMZAAN, Farina KHATTAK, Raheela AKHTAR

Effects of acetic acid supplementation in broiler chickens orallychallenged with Salmonella Pullorum

This study was conducted to investigate the comparative efficacy of three different concentrations of acetic acid on broiler chickens experimentally challenged with Salmonella enterica serovar Pullorum (S. Pullorum). A total of 360 birds were divided into five groups (A E). Group A served as the unchallenged, untreated control. All the birds in groups B E were challenged with 4 × 104 cfu/mL of S. Pullorum. Birds in groups C, D, and E were respectively treated with three different concentrations, 0.5%, 1%, and 1.5%, of acetic acid. Pathological examination revealed congested and hemorrhagic liver, hepatitis and necrotic areas in the liver, hydropericardium, focal necrosis in the spleen, epithelial desquamation of the intestinal mucosa, and congested lungs and hazy appearance of air sacs in birds challenged with S. Pullorum. Acetic acid supplementation (1%) helped to reduce the number and severity of these gross and histopathological changes. Counts of S. Pullorum in cecal digesta were significantly reduced with increasing concentrations of acetic acid (P < 0.05). However, acetic acid supplementation at a higher rate (1.5%) also showed adverse effects in terms of higher percentage of diarrhea and bad growth performance in birds challenged with S. Pullorum. Among the three different concentrations, 1% acetic acid supplementation showed partially protective effects by showing better growth performance, lower feed conversion ratios (P < 0.05), and lower rates of gross and histopathological changes.

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1. Subtain SM, ZI Chaudhry, Anjum AA, Maqbool A, Sadique U. Study on pathogenesis of low pathogenic avian influenza virus H9 in broiler chickens. Pak J Zool 2011; 43: 9991008.
2. Chappell L, Kaiser P, Barrow P, Jones MA, Johnston C, Wigley P. The immunobiology of avian systemic salmonellosis. Vet Immunol Immunop 2009; 128: 5359.
3. Wigley P, Berchieri A Jr, Page K, Smith AL, Barrow PA. Salmonellaenterica serovar Pullorum persists in splenic macrophages and in the reproductive tract during persistent disease-free carriage in chickens. Infect Immun 2001; 69: 78737879.
4. Williams P, Losa R. The use of essential oils and their compounds in poultry nutrition. World Poultry 2001; 17: 1415.
5. Van Immerseel F, Cauwerts K, Devriese LA, Haesebrouck F, Ducatelle R. Feed additives to control Salmonella in poultry. World Poultry Sci J 2002; 58: 431443.
6. Jeong JS, Kim IH. Effect of Bacillussubtilis C-3102 spores as a probiotic feed supplement on growth performance, noxious gas emission, and intestinal microflora in broilers. Poultry Sci 2014; 93: 30973103.
7. Iba AM, Berchieri A Jr. Studies on the use of formic acid-propionic aid mixture (Bio-AddTM) to control experimental Salmonella infection in broiler chickens. Avian Pathol 1995; 24: 304311.
8. Alshawabkeh K, Tabbaa JM. Using dietary propionic acid to limit Salmonella Gallinarum colonization in broiler chicks. Asian-Aust J Anim Sci 2002; 15: 243246.
9. Van Immerseel F, De Buck J, Boyen F, Bohez L, Pasmans F, Volf Rychlik I, Haesebrouck F, Ducatelle R. Medium chain fatty acid decreases colonization and invasion through hilA suppression shortly after infection of chickens with Salmonella entericaserovar Enteritidis. Appl Environ Microbiol 2004; 70: 35823587.
10. Al-Tarazi YH, Alshawabkeh K. Effect of dietary formic and propionic acids on Salmonella Pullorum shedding and mortality in layer chicks after experimental infection. J Vet Med 2003; 50: 112117.
11. Bancroft JD, Stevens A, Turner DR. Theory and Practice of Histological Technique. 4th ed. London, UK: Churchill Livingstone; 1996.
12. Al-Kassi AG, Mohssen MA. Comparative study between single organic acid effect and synergistic organic acid effect on broiler performance. Pakistan Journal of Nutrition 2009; 8: 896899.
13. Haider MG, Chowdhury EHK, Hossain A, Rahman MT, Song MS, Hossain MM. Experimental pathogenesis of pullorum disease in chicks by local isolate of Salmonellaenterica serovar. Enteric subspecies Pullorum in pullets in Bangladesh. Korean Journal of Poultry Science 2009; 35: 341350.
14. Vandeplas S, Dauphin RD, Thiry C, Beckers Y,Welling GW, Thonart P, Théwis A. Efficiency of a Lactobacillus plantarum-xylanase combination on growth performances, microflora populations, and nutrient digestibilities of broilers infected with Salmonella Typhimurium. Poultry Sci 2009; 88: 16431654.
15. Marcq C, Cox E, Szalo IM, Théwis A, Beckers Y. SalmonellaTyphimurium oral challenge model in mature broilers: bacteriological, immunological, and growth performance aspects. Poultry Sci 2011; 90: 5967.
16. Mroz, Z, Jongbloed AW, Partanen KH, Vermin K, Kemme PA, Kogut J. The effects of calcium benzoate in diets with or without organic acids on dietary buffering capacity, apparent digestibility, retention of nutrients and manure characteristics in swine. J Anim Sci 2000; 78: 26222632.
17. Omogbenigun FO, Nyachti CM, Solminski BA. The effect of supplementing microbial phytase and organic acids to corn-soybean based diet fed to early weaned pigs. J Anim Sci 2003; 81: 18061813.
18. Bohez L, Gantois I, Ducatelle R, Pasmans F, Dewulf J, Haesebrouck F, Van Immerseel F. The Salmonella Pathogenicity Island 2 regulator ssrA promotes reproductive tract but not intestinal colonization in chickens. Vet Microbiol 2008; 126: 216224.
19. Wang LC, Zhang TT, Wen C, Jiang ZY, Wang T, Zhou YM. Protective effects of zinc-bearing clinoptilolite on broilers challenged with Salmonella Pullorum. Poultry Sci 2008; 91: 18381845.
20. Haider MG, Chowdhury AKM, Hossain MM. Experimental pathogenesis of pullorum disease in chicks by local isolate of Salmonella Pullorum in Bangladesh. Journal of the Bangladesh Agricultural University 2008: 10: 8794.
21. Berchieri JR, Barrow PA. Reduction in incidence of experimental fowl typhoid by incorporation of a commercial formic acid preparation (Bio-Add) into poultry feed. Poultry Sci 1996; 75: 339341.
22. Carina Audisio M, Oliver G, Apella MC. Protective effect of Enterococcusfaecium J96, a potential probiotic strain, on chicken infected with Salmonella Pullorum. J Food Prot 2000; 63: 13331337.
23. Totton CS, Farrar MA, Wilkins W, Bucher O, Waddell AL, Wilhelm JB, McEwen AS, Rajic A. A systematic review and meta-analysis of the effectiveness of biosecurity and vaccination in reducing Salmonella spp. in broiler chickens. Food Res Int 2011; 45: 617627.
24. Soleimani FA, Zulkifli I, Hair-Bejo M, Omar RA, Raha RA. The role of heat shock protein 70 in resistance to Salmonellaenteritidis in broiler chickens subjected to neonatal feed restriction and thermal stress. Poultry Sci 2011; 91: 340345.
25. Hossain M, Chowdhury E, Islam M, Haider M Hossain, M. Avian Salmonella infection: isolation and identification of organisms and histopathological study. Bangladesh Journal of Veterinary Medicine 2011; 4: 712.
26. Pinheiro LA, De Oliveira GH, Berchieri A Jr. Experimental Salmonella enterica serovar Pullorum infection in two commercial varieties of laying hens. Avian Pathol 2001; 30: 129133.
27. Filho-Lima J, Vieira E, Nicoli J. Antagonistic effect of Lactobacillus acidophilus, Saccharomyces boulardii and Escherichia coli combinations against experimental infections with Shigella flexneri and Salmonella enteritidis subsp. typhimurium in gnotobiotic mice. J Appl Microbiol 2000; 88: 365370.
28. Hargis B, Caldwell D, Brewer R, Corrier D, Deloach J. Evaluation of the chicken crop as a source of Salmonella contamination for broiler carcasses. Poultry Sci 1995; 74: 15481552.
29. Borsoi A, Santos LRD, Diniz GC, Salle C, Moraes H, Nascimento VP. Salmonella fecal excretion control in broiler chickens by organic acids and essential oils blend feed added. Revista Brasileira de Ciência Avícola 2011; 13: 6569.
30. McHan F, Shots B. Effect of short chain fatty acids on the growth of Salmonella typhimurium in an in vitro system. Avian Dis 1993; 37: 396398.
31. Schulz I, Ströver F, Ullrich KJ. Lipid soluble weak organic acid buffers as substrate for pancreatic secretion. Pflügers Arch 1971; 323: 121140.
32. Russell JB, Diez-Gonzalez F. The effects of fermentation acids on bacterial growth. Adv Microb Physiol 1998; 39: 205234.
33. Byrd JA, Hargis BM, Caldwell DJ, Bailey RH, Herron KL, McReynolds JL, Brewer RL, Anderson RC, Bischoff KM, Callaway TR et al. Effect of lactic acid administration in the drinking water during pre-slaughter feed withdrawal on Salmonella and Campylobacter contamination of broilers. Poultry Sci 2001; 80: 278283.