ÖZGÜR BAYTUT, Cem Tolga GÜRKANLİ, İBRAHİM ÖZKOÇ, ARİF GÖNÜLOL

Assessing 18S rDNA diversity of the chlorophytes among various freshwaters of the Central Black Sea Region

Bu çalışmada Orta Karadeniz Bölgesindeki çeşitli tatlı su habitatlarından izole edilen tek hücreli klorofitlerin çeşitliliği moleküler filogenetik yöntemler ile belirlenmiştir. Çeşitliliği belirlemek için, su örnekleri Cernek Lagünü (Kızılırmak Deltası- Samsun), Kürtün nehir ağzı (Samsun), Sarıkum Gölü (Sinop) ve Boyabat Göletini (Sinop) kapsayan tatlı su habitatlarından alınmıştır. Aksenik kültürler, zenginleştirilmiş kültürlerden katı besi ortamlarına pasajlama yapılarak elde edilmiştir. İzolatların karakterizasyonu için morfolojik gözlemlerin yanı sıra, nükleer ribozomal DNA küçük alt biriminin (18S rDNA) nükleotid dizilerine dayalı filogenetik analizler kullanılmıştır. Yakın ilişkili tek hücreli klorofitler üzerinde yapılan mikroskobik gözlemlerde izolatlar arasında önemli farklılıklar görülmemiştir. Filogenetik analizlerin sonucu olarak izolatlar Chlamydomonadaceae (izolatlar 103I2 ve C301), Chlorellaceae (izolat B4Riv) ve Scenedesmaceae (izolatlar SIN-CON ve N603) familyaları ile ilişkili üç farklı soy hattında yer almıştır. Bu çalışmadaki sonuçlarımız, klorofitlerin doğru teşhisleri için morfolojik gözlemlerin yetersizliğini ve aynı zamanda moleküler metotların gere kliliğini açık şekilde göstermiştir. Buna ek olarak uluslar arası veritabanlarına Türkiye tatlı sularından yukarıda belirtilen klorofit familyalarına ait ilk 18S rDNA haplotiplerini sağlamıştır.

Orta Karadeniz Bölgesindeki çeşitli tatlı sularda 18S rDNA klorofit çeşitliliğinin belirlenmesi

In this study, diversity of unicellular chlorophytes isolated from various freshwater habitats in the Central Black Sea Region were assessed by the molecular phylogenetic methods. In order to determine diversity , water samples were collected from freshwater habitats including Cernek Lagoon (Kızılırmak Delta -Samsun), Kürtün Estuary (Samsun), Sarıkum Lagoon (Sinop) and a freshwater pond in Boyabat (Sinop). Axenic cultures were obtained with re-streaking the isolates from enrichment cultures on solid growth media. For characterisation of isolates, phylogenetic analyses depending on nucleotide sequences of small subunit of nuclear ribosomal DNA (18S rDNA) was used besides morphological observations. In morphological o bservations upon closely related unicellular chlorophytes, there were no significant morphological differences among the isolates. As a result of phylogenetic analyses, our isolates were appeared in three distinct lineages which were related with Chlamydomonadaceae (isolates 103I2 and C301), Chlorellaceae (isolate B4Riv) and Scenedesmaceae (isolates SIN -CON and N603) families. Our results in this study clearly indicates the deficiency of the morphological observations and also the necessity of the molecular methods for true identification of chlorophytes. Additionally, it provides the first 18S rDNA haplotypes of chlorophyte families stated above from Turkish freshwaters to international databases.

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