Saltuk Buğrahan CEYHUN, ABDULKADİR ÇİLTAŞ

Determination of genetic variations of five different indigenous trout species in Turkey (salmo trutta sp. L.) by microsatellite markers

Bu çalışmada beş farklı alabalık poulasyonunun genotipik farklılığı mikrosatelit markırlar kullanılarak karşılaştırıldı. Analiz edilen balıklardan; Balıklı Göl (BLK) ve Abant Gölü (ABN) gölde, ve Fırat Havzası (FRT), Aras Havzası (ARS) ve Çoruh Havzası (CRH) nehirde yaşamaktadırlar. Her bir lokustaki allel sayısı Single Strand Conformation Polymorphism (SSCP) yöntemi vasıtasıyla belirlendi. 10 mikrosatelit bölgede toplam 62 allel gözlendi. Faktöriyel uyum analizi sonuçlarına göre, populasyonların tamamının birbirlerinden farlı gruplar oluşturduğu saptanmıştır. Tüm lokuslar ele alındığında gözlenen (Ho) ve beklenen (He) heterozigotluk değerleri sırasıyla 0,602 -0,668 ve 0,570 -0,670 arasında değişmiştir. FRT populasyonunda Ho değeri He değerinden önemli derecede yüksek çıkmıştır (P

Türkiye de ki beş farklı yerli alabalık türünün genetik varyasyonunun mikrosatelit markırlar ile belirlenmesi

In this study, the genotypic differences of five different trout populations were compared using microsatellite markers. Fish analyzed were; Balıklı Lake (BLK) and Abant Lake (ABN) found in lakes, and Fırat Basin (FRT), Aras Basin (ARS), Çoruh Basin (CRH) found in rivers. Allele numbers in each locus were determined by application of Single Strand Conformation Polymorphism (SSCP) to the microsatellite sections. Totally 62 alleles were observed from 10 microsatellite loci. According to the results from factorial correspondence analysis, it was determined that all of the popula tions formed different groups from each other. Considering all loci, observed heterozygosity (H o) and expected heterozygosity (H e), values changed from 0.602 -0.668 and 0.570 -0.670, respectively. Ho value was significantly (P<0.01) higher than He value of p opulations of FRT. Fixation index for subpopulations (FIS) values were ranged from 0.069 to -0.173 (BLK to FRT) in populations, and the overall average diversity between populations (FST ) value of the all loci was calculated as 0.0790. According to FST values calculated among populations, genetic distance was found to be significant ( P<0.05) among four populations. It was determined that four of five populations were at the Hardy -Weinberg equilibrium (HWE). Considering genetic distance in populations, it was observed that the distance was shorter in lake forms compared to River forms.

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