Muhammet GAFFAROĞLU, MURADİYE KARASU AYATA, SEVGİ ÜNAL KARAKUŞ, ATİLLA ARSLAN

Chromosomal studies of two different populations (Turkey) of Luciobarbus escherichii (Steindachner, 1897)

Luciobarbus escherichii (Steindachner, 1897)nin iki farklı populasyonunda (Kızılırmak ve Sakarya Nehri) kromozomal araştırmalar yapıldı. Metafaz kromozomları böbrek hücrelerinden elde edildi. L. escherichiinin diploit kromozom sayısı 2n=100; kromozom morfolojisi ise 7 çift metasentrik, 22 çift submetasentrik ve 21 çift subtelo -akrosentrik olarak belirlendi. Kol sayısı (NF) 158 olarak hesaplandı. C-bantlama ile kromozomların sentromerinde konstitütif heterokromatin bölge gözlendi. Ayrıca 2 çift submetasentrik kromozomun kısa kollarının ucunda NOR tespit edildi. Bu çalışmanın Anadolu cyprinidlerinin sitogenetiğine katkı sağlayacağı düşünülmektedir.

Luciobarbus escherichii (Steindachner, 1897) nin iki farklı populasyonunda (Türkiye) kromozomal araştırmalar

This study was carried out on the chromosomal features of two populations (Kızılırmak and Sakarya Rivers) belonging to Luciobarbus escherichii (Steindachner, 1897). Metaphase chromosomes were obtained from kidney cells. The diploid chromosome number was 2n=100, consisting of 7 pairs of metacentric, 22 pairs of submetacentric and 21 pairs of subtelo - acrocentric chromosomes. The arm number (NF) was 158. Constitutive heterochromatin regions with C -banding were determined on the centromeres of chromosomes. NOR was observed on the short arms of 2 pairs of submetacentric ch romosomes. This study is a contribution to cytogenetics of Anatolian cyprinids.

PDF

___

Arai, R. 2011. Fish karyotypes A Check List. Springer, Japan, 340 pp.
Collares-Pereira, M.J. 1992. In vivo direct chromosome preparation (Air Drying Technique), 1 st Int. Workshop on fish cytogenetic techniques , Concarneau -France, 14 -24 September pp: 15 -9.
Comber, S.C.L., Smith, C. 2004. Polyploidy in ﬁshes: patterns and processes. Biological Journal of the Linnean Society, 82: 431 442.
Darestani, S.P., Lakeh, A.A.B., Kiabi, B.H.A. 2006. Karyological study of Barbus capito , Barbus mursa and two populations of Capoeta c apoeta from Northern Iran. Iranian Journal of Natural Resources, 58(4): 831 -842.
Değer, D., Ünlü, E., Gaffaroğlu, M. 2011a. Dicle Nehrinde Yaşayan C. luteus (Heckel, 1843) Türünün Karyolojik Özellikleri. X. Ekoloji ve Çevre Kongresi, 4 -7 Ekim, Çanakkale.
Değer, D., Ünlü, E., Gaffaroğlu, M. 2011b. Kosswigobarbus kosswigi (Ladiges, 1960) türünün karyotip analizi. FABA, 7 -9 Eylül, Samsun.
Eschmeyer, W.N., Fong , J.D. 2013. Catalog of Fishes Online. http://research.calacademy.org/redirect?url=http://rese archarchive.calacademy .org/research/Ichthyology/cata log/SpeciesByFamily.asp . (Accessed February 22, 2013).
Ergene, S., Kuru, M., Çavaş, T. 1993. Karyological analysis of Barbus plebejus lacerta (Heckel, 1843). II. Uluslararası Kızılırmak Fen Bilimleri Kongresi, 426 - 433s.
Fiste r, S. Cakic, P, and Kataranovski, D. 1999. Karyotype analysis of Barbus barbus L. and Barbus peloponnensius V. (Cyprinidae) and frequencies of breaks and gap -type structural chromosome changes in fishes from the river Vapa. Acta Veterinaria- Beograd. 49(5 -6 ): 385 -392.
Gaffaroğlu, M., Yüksel, E. 2009. Constitutive heterochromatin in Acanthobrama marmid and Cyprinion macrostomus (Osteichthyes, Cyprinidae). Kafkas University V eterinarian Faculty , 15 (2): 169 - 72.
Gaffaroğlu, M., Yüksel, E. and Rab, P. 2006. Note on the karyotype and NOR phenotype of leuciscine fish Acanthobrama marmid (Osteichthyes, Cyprinidae). Biologia, 61 (2): 207 -209. doi:10.2478/s11756 -006 - 0031 -y.
Güçlü, S. S., Ertan, Ö. O., Küçük, F. 2011. Anadoludaki Luciobarbus (Pisces: Cyprinidae) Tür lerinin Taksonomik ve Zoocografik Özellikleri. II. Türkiye Sulak Alanlar Kongresi, Kırşehir, 44. Sayfa.
Howell, W. M., Black, D. A. 1980. Controlled Silver Staining of Nucleolus Organizer Regions with a Protective Colloidal Developer: a 1 Step Method . Experientia , 36: 1014 -5.
Kaya, F. 2009. Göksu Nehrinde yaşayan bazı ekonomik balıkların karyolojilerinin incelenmesi. PhD Thesis . Kılıç -Demirok, N. 2000. Dicle Su Sisteminde yaşayan bazı cyprinid tür ve alttürlerinin kromozomları üzerine çalışmalar. PhD Thesis.
Levan, A., Fredga, K., Sandberg, A.A. 1964. Nomenclature For Centromeric Position On Chromosomes. Hereditas, 52: 201 -20.
Naran, D., Skelton, P. H., Villet, M. H. 2006. Karyology of the redfin minnows, genus Pseudobarbus Smith, 1841 (Teleostei: Cyprinid ae): one of the evolutionarily tetraploid lineages of South African barbines. African Zoology, 41(2): 178 182.
Rab, P. 1981. Karyotypes of two African Barbels Barbus bariloides and Barbus holotaenia . Folia Zoologica, 30 (2): 181 -190.
Rab, P., Collares-Pere ira M. J. 1995. Chromosomes of European Cyprinid fishes (Cyprinidae, Cypriniformes): A review. Folia Zoologica , 44 (3): 193 -214.
Rab, P., Karakousis, Y., Rabova, M. 1996. Karyotype, NOR phenotype and C-banding study of Barbus cyclolepis from Greece. Folia Zoologica, 45 (1): 77 -83.
Rab, P., Machordom, A., Perdices, A., Guegan, J. F. 1995. Karyotypes of three __________small>> Barbus species (Cyprinidae) from Republic of Guinea (Western Africa) with a review on karyology of African small Barbus. Caryologia, 48(3 -4): 299 -307.
Rab, P., Ozouf-Costaz, C., Berrebi, P. 1993. Karyotypes, distribution of centromeric heterochromatin and polymorphism of NORs in Barbus meridionalis from Southern France and Eastern Slovakia: preliminary results. Chaiers d Ethologie, 13 (2): 195 -198.
Sumner, A.T. 1972. A simple Technique for Demonstrating Centromeric Heterochromatin. Experimental Cell Research, 75 : 304 -6.