Ataç UZEL, Erdal BEDİR, E. Esin Hameş KOCABAŞ

Prevalence of hermoactinomyces thalpophilus and T. sacchar i strains with biotechnological potential at hot springs and soils from West Anatolia in Turkey

Türkiye, Batı Anadolu’dan alınan sıcak su kaynağı sedimenti ve toprak örnekleri termofilik aktinomiset mevcud- iyeti bakımından araştırıldı. İzolasyon, 55 °C’de Aktinomiset izolasyon agar (AIA), Nişasta kazein agar ve Gliserol maya özütü agar kullanılarak yapıldı. İzolatların hücre dışı proteaz aktiviteleri %1 kazein içeren AIA kullanılarak kolonileri çevreleyen hidroliz zonlarına göre tarandı. İzolatların antimikrobiyal aktiviteleri de çizgi plaka yöntemi ile bir seri test bakterisine karşı tarandı. İzolatların tanılanmaları kültürel fizyolojik karakteristiklere ve 16S rDNA dizi benzerliklerine göre yapıldı. Altmış yedi termofilik aktinomiset izolatı Thermoactinomyces thalpophilus ve T. sacchari türleri içinde sınıflandırıldı. Altmış iki izolatın (%92,5) hücre dışı proteaz üreticisi olduğu ve 38 izolatın da (%56,7) metisillin dirençli Staphylococcus aureus ve Enterococcus faecalis’e karşı aktif oldukları belirlendi. Bu sonuçlardan hareketle, Türkiye, Batı Anadolu’daki sıcak su kaynakları ve topraklarındaki biyoçeşitliliğin bir parçası olan termofilik aktinomisetlerin yeni en- zimler ve antimikrobiyal maddeler için umut vaat edici kaynaklar olabilecekleri öngörülmektedir.

Türkiye Batı Anadolu sıcak su kaynakları ve topraklarındaki biyoteknolojik potansiyele sahip hermoactinomyces thalpophilus ve T. sacchar i suşlarının prevalansı

Hot spring sediment and soil samples from West Anatolia in Turkey were investigated for the occurrence of thermophilic Actinomycetes. Isolation was done using Actinomycetes isolation agar (AIA), starch casein agar and glycerol yeast extract agar at 55 °C. Extracellular protease activity of the isolates were screened by using AIA plus 1% casein according to the hydrolysis zones surrounding the colonies. Antimicrobial activities of the isolates were also screened by using streaked plate method against a panel of test bacteria. Identification of the isolates was made by cultural, physiological characteristics and 16S rDNA sequence similarity. Sixty-seven thermophilic Actinomycetes isolates were classified in Thermoactinomyces thalpophilus and T. sacchari species. Among these, 62 isolates (92.5%) were found to be extracellular protease producers and 38 isolates (56.7%) were found active against methicillin resistant Staphylococcus aureus and Enterococcus faecalis. Based on these results, we suggest that the thermophilic actinomycetes, which are a part of the biodiversity of the hot springs and soils from West Anatolia in Turkey, are promising sources for novel enzymes and antimicrobial compounds.

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