Bilge ŞENER, İlkay ORHAN, Berrin ÖZÇELİK

Evaluation of antibacterial, antifungal, antiviral, and antioxidant potentials of some edible oils and their fatty acid proiles

Bu çalışmada, Corylus avellana L. (fındık), Arachis hypogea L. (yerfıstığı), Pinus pinea L. (çam fıstığı) ve Juglans regia L. (ceviz) meyvalarından elde edilen yağlar, antioksidan aktiviteleri için 2,2-difenil-1-pikrilhidrazil (DPPH) radikaline karşı, antimikrobiyal aktiviteleri için, inhibisyonun minimum inhibitör konsantrasyon (MIK) olarak ifade edildiği mikrodilüsyon yöntemi ile Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Klebsiella pneumoniae, Acinetobacter baumannii, Staphylococcus aureus ve Enterococcus faecalis’in standart ve izole suşları ile Candida albicans ve C. parapsilosis adlı mantarlara karşı test edilmişlerdir. Ayrıca yağların antiviral aktivitesi Herpes simplex (HSV) and Parainfluenza (PI-3) kullanılarak tayin edilmiştir. Yağ asitleri analizi ise GC-MS tekniği ile gerçekleştirilmiştir. Fındık ve ceviz yağları 100 mg mL-1 ’de en iyi antioksidan aktiviteyi (sırasıyla % 73,58 ve 65,10) gösterirken, en güçlü anti-PI-3 etkiye de (16-

Bazı yenilebilen yağların antibakteriyel, antifungal, antiviral ve antioksidan potansiyellerinin değerlendirilmesi ve yağ asitleri proilleri

In the current study, the oils obtained from the nuts of Corylus avellana L. (hazelnut), Arachis hypogea L. (peanut, groundnut), Pinus pinea L. (umbrella nut), and Juglans regia L. (walnut) were tested for their antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and antimicrobial activity against the standard and isolated strains of Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Klebsiella pneumoniae, Acinetobacter baumannii, Staphylococcus aureus, and Enterococcus faecalis as well as the fungi Candida albicans and C. parapsilosis by microdilution method, in which inhibition was expressed as minimum inhibition concentrations (MICs). Moreover, antiviral activity of the oils was determined using Herpes simplex (HSV) and Parainfluenza (PI-3). Their fatty acid analyses were performed by GC-MS technique. While the hazelnut and walnut oils showed the best antioxidant activity at 100 mg mL-1 (73.58% and 65.10%, respectively), the walnut oil had the most potent anti-PI-3 effect (16- < 0.25 μg mL-1 ). The oils displayed inhibitory potentials towards the isolated strains of E. coli, S. aureus, and E. faecalis at 4, 8, and 8 μg mL-1concentrations, respectively.

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1. Burton JW, Miller JF, Vick BA et al. Altering fatty acid composition in oil seed crops. Adv Agron 84: 273-306, 2004.
2. Clinical and Laboratory Standards Institute (CLSI; formerly NCCLS), National Committee for Clinical Laboratory Standards, Performance standards for antimicrobial susceptibility testing; 16 th Informational supplement. CLSI document M7-A7, 940 West Valley Road, Wayne, Pennsylvania, USA, 2008: 19087-1898.
3. Clinical and Laboratory Standards Institute (CLSI). Reference method for broth dilution antifungal susceptibility testing of yeast; approved standard-3 rd edition. CLSI document M27-A3, (ISBN 1-56238-666-2) Clinical and Laboratory Standards Institute, 940 West Valley Road, Suite 1400, Wayne, Pennsylvania, USA, 2008: 19087-1898.
4. Özçelik B, Aslan M, Orhan I et al. Antibacterial, antifungal, and antiviral activities of the lipophylic extracts of Pistacia vera L. Microbiol Res 160: 159-164, 2005.
5. Özçelik B, Orhan I, Toker G. Antiviral assessment against Herpes simplex virus and Parainfluenza-3 virus in Vero cell line and Madin-Darby bovine kidney cells and antimicrobial activity of some flavonoids. Z Naturforsch 61c: 632-638, 2006.
6. Deliorman-Orhan D, Orhan N, Özçelik B et al. Biological activities of Vitis vinifera L. leaves. Turk J Biol 33: 341-348, 2009.
7. Ramadan MF, Moersel JT. Screening of antiradical action of vegetable oils. J Food Comp Anal 19: 838-842, 2006.
8. Morrison WR, Smith LM. Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron trifluoride- methanol. J Lipid Res 5: 600-608, 1964.
9. McKellar RC, Paquet A, Ma CY. Antimicrobial activity of fatty N-acylamino acids against Gram-positive foodborne pathogens. Food Microbiol 9: 67-76, 1992.
10. Isaacs CE, Litou RE, Thormar H. Antimicrobial activity of lipids added to human milk, infant formula, and bovine milk. J Nutr Chem 6: 362-366, 1995.
11. Kankaanpaa PE, Salminen SJ, Isolauri E et al. The influence of polyunsaturated fatty acids on probiotic growth and adhesion. FEMS Microbiol Lett 194: 149-153, 2001.
12. Sprong RC, Hulstein MFE, Van Der Meer R. Bovine milk fat components inhibit food-borne pathogens. Int Dairy J 12: 209- 215, 2002.
13. Benkendorff K, Davis AR, Rogers CN et al. Free fatty acids and sterols in the benthic spawn of aquatic mollusks and their associated antimicrobial properties. J Exp Marine Biol Ecol 316: 29-44, 2005.
14. Knapp HR, Melly MA. Bactericidal effects of polyunsaturated fatty acids. J Infec Dis 154: 84-94, 1986.
15. Farrington M, Brenwald N, Haines D et al. Resistance to desiccation and skin fatty acids in outbreak strains of methicillin-resistant Staphylococcus aureus. J Med Microbiol 36: 56-60, 1992.
16. Dilika F, Brenner PD, Meyer JJM. Antibacterial activity of linoleic and oleic acids isolated from Helichrysum pedunculatum: a plant used during circumcision rites. Fitoterapia 71: 450-452, 2000.
17. Zheng CJ, Yoo JS, Lee TG et al. Fatty acid synthesis is a target for antibacterial activity of unsaturated fatty acids. FEBS Lett 579: 5157-5162, 2005.
18. Waller RF, Keeling PJ, Donald RG et al. Nuclear-encoded proteins target to the plastid in Toxoplasma gondii and Plasmodium falciparum. Proc Nat Acad Sci USA 95: 12352- 12357, 1998.
19. Galbraith H, Miller TB, Paton AM et al. Antibacterial activity of long-chain fatty acids and reversal with Ca, Mg, ergocalciferol and cholesterol. J Appl Bacteriol 34: 803-813, 1971.
20. Glass KA, Johnson EA. Antagonistic effect of fat on the botulinal activity of food preservatives and fatty acids. Food Microbiol 21: 675-682, 2004.
21. Parcerisa J, Richardson DG, Rafecas M et al. Fatty acid, tocopherol and sterol content of some hazelnut varieties (Corylus avellana L.) harvested in Oregon (USA). J Chrom A 805: 259-268, 1998.
22. Oliveira I, Sousa A, Sa Morais J et al. Chemical composition and antioxidant and antimicrobial activities of three hazelnut (Corylus avellana L.) cultivars. Food Chem Toxicol 46: 1801- 1807, 2008.
23. Nergiz C, Dönmez I. Chemical composition and nutritional value of Pinus pinea L. seeds. Food Chem 85: 365-368, 2004.
24. Nasri N, Khaldi A, Fady B et al. Fatty acid from seeds of Pinus pinea L.: Composition and population profiling. Phytochemistry 66: 1729-1735, 2005.
25. Köksal AI, Artik N, Şimşek A et al. Nutrient composition of hazelnut (Corylus avellana L.) varieties cultivated in Turkey. Food Chem 99: 509-515, 2006.
26. Davidson PM, Sofos JN, Branen AL. Antimicrobials in foods. 3 rd ed. CRC Press (Taylor & Francis Group). Boca Raton; 2005: pp. 346-347.
27. Henry GE, Momin RA, Nair MG et al. Antioxidant and cyclooxygenase activities of fatty acids found in food. J Agric Food Chem 50: 2231-2234, 2002.
28. Shiota M, Tatsumi K. Effect of sucrose ester of fatty acid on the antioxidant activity of milk products on fish oil oxidation. J Food Sci 67: 547-552, 2002.
29. Ceffarelli G, D’abrosa B, Fiorentina A et al. Isolation, characterization and antioxidant activity of E- and Z-p- coumaryl fatty acid esters from cv. Annurca apple fruits. J Agric Food Chem 53: 3525-3529, 2005.
30. El-Din B, Nariman K, Stanley T. Concentration-dependent antioxidant activities of conjugated linoleic acid and α- tocopherol in corn oil. J Sci Food Agric 87: 2715-2720, 2007.
31. Hur SJ, Park GB, Joo ST. Biological activities of conjugated linoleic acid (CLA) and effects of CLA on animal products. Livestock Sci 110: 221-229, 2007.
32. Baldioli M, Servili M, Perretti G et al. Antioxidant activity of tocopherols and phenolic compounds of virgin olive oil. JAOCS 73: 1589-1593, 1996.
33. Mambro VMD, Azzolini AECS, Valim YML et al. Comparison of antioxidant activities of tocopherols alone and in pharmaceutical formulations. Int J Pharmaceutics 262: 93-99, 2003.
34. Li L, Tsao R, Yang R, Kramer JK et al. Fatty acid profiles, tocopherol contents, and antioxidant activities of heartnut (Juglans ailanthifolia var. cordiformis) and Persian walnut (Juglans regia L.). J Agric Food Chem 55: 1164-1169, 2007.